公开(公告)号：US11747271B2

公开(公告)日：2023-09-05

申请号：US16328208

申请日：2017-08-17

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Gabor Jarvas ,  Marton Geza Szigeti ,  Andras Guttman ,  Jeff Chapman

IPC: G01N21/33 ,  G01N33/53 ,  G16C20/20 ,  G01N21/64 ,  G01N27/447 ,  G01N30/04 ,  G01N30/88

CPC classification number: G01N21/33 ,  G01N33/5308 ,  G16C20/20 ,  G01N21/6402 ,  G01N27/44726 ,  G01N2030/045 ,  G01N2030/8836

Abstract: A separation device receives a known or unknown glycan that is co-injected with three different oligomers maltooligosaccharide (MOL). A detector measures the separated glycan and the separated three different oligomers as intensity peaks that are a function of migration time. The migration times of a plurality of other oligomers of MOL are calculated from the migration times of the three different oligomers. Glucose unit (GU) values for the intensity peaks of the separated glycan are calculated by comparing their migration times to the calculated migration times of the plurality of other oligomers of MOL. The processor identifies the structure of the glycan by comparing the calculated GU values of the intensity peaks of the separated glycan to a database of GU values for known glycan structures.

公开(公告)号：US20190234867A1

公开(公告)日：2019-08-01

申请号：US16328208

申请日：2017-08-17

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Gabor Jarvas ,  Marton Geza Szigeti ,  Andras Guttman ,  Jeff Chapman

IPC: G01N21/33 ,  G01N33/53 ,  G16C20/20

CPC classification number: G01N21/33 ,  G01N21/6402 ,  G01N27/44726 ,  G01N33/5308 ,  G01N2030/045 ,  G01N2030/8836 ,  G16C20/20

Abstract: A separation device receives a known or unknown glycan that is co-injected with three different oligomers maltooligosaccharide (MOL). A detector measures the separated glycan and the separated three different oligomers as intensity peaks that are a function of migration time. The migration times of a plurality of other oligomers of MOL are calculated from the migration times of the three different oligomers. Glucose unit (GU) values for the intensity peaks of the separated glycan are calculated by comparing their migration times to the calculated migration times of the plurality of other oligomers of MOL. The processor identifies the structure of the glycan by comparing the calculated GU values of the intensity peaks of the separated glycan to a database of GU values for known glycan structures.