公开(公告)号：US11747271B2

公开(公告)日：2023-09-05

申请号：US16328208

申请日：2017-08-17

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Gabor Jarvas ,  Marton Geza Szigeti ,  Andras Guttman ,  Jeff Chapman

IPC: G01N21/33 ,  G01N33/53 ,  G16C20/20 ,  G01N21/64 ,  G01N27/447 ,  G01N30/04 ,  G01N30/88

CPC classification number: G01N21/33 ,  G01N33/5308 ,  G16C20/20 ,  G01N21/6402 ,  G01N27/44726 ,  G01N2030/045 ,  G01N2030/8836

Abstract: A separation device receives a known or unknown glycan that is co-injected with three different oligomers maltooligosaccharide (MOL). A detector measures the separated glycan and the separated three different oligomers as intensity peaks that are a function of migration time. The migration times of a plurality of other oligomers of MOL are calculated from the migration times of the three different oligomers. Glucose unit (GU) values for the intensity peaks of the separated glycan are calculated by comparing their migration times to the calculated migration times of the plurality of other oligomers of MOL. The processor identifies the structure of the glycan by comparing the calculated GU values of the intensity peaks of the separated glycan to a database of GU values for known glycan structures.

公开(公告)号：US20240159703A1

公开(公告)日：2024-05-16

申请号：US18548708

申请日：2022-02-28

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Tingting Li ,  Handy Yowanto ,  Andras Guttman

IPC: G01N27/447 ,  G01N33/569

CPC classification number: G01N27/44726 ,  G01N27/44791 ,  G01N33/56983 ,  G01N2333/155 ,  G01N2458/00

Abstract: Methods and kits for quantifying a viral species are presently claimed and described. The method includes the steps of preparing at least one labeled viral protein component by incubating a detectable dye with a virial species in the presence of sodium dodecyl sulfate (SDS); loading the labeled viral protein component onto a capillary electrophoresis (CE) capillary, wherein the CE capillary is filled with a buffer comprising a polymer matrix; applying a separation voltage to the CE capillary; detecting at least one labeled viral protein component with a detector, thereby producing a corresponding set of values; and quantifying a protein of interest in the viral protein component using the corresponding set of values.

公开(公告)号：US20210082676A1

公开(公告)日：2021-03-18

申请号：US17041330

申请日：2019-05-23

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Andras Guttman ,  Mate Szarka

IPC: H01J49/00 ,  G01N21/64 ,  H01J49/16 ,  G01N30/72

Abstract: Fluorescently labeled molecules of a sample are quantitated in an ion source device of a mass spectrometer. An illumination source device to illuminates at least a first portion of a sample. The sample is illuminated to excite fluorescently labeled molecules of the sample as it is being ionized in the ion source device. A two-dimensional digital image detector measures an image of at least a second portion of the illuminated first portion of the sample at each time interval of a plurality of time intervals. Each measured image at each of the plurality of time intervals is stored in a memory device. A trace of the intensity of the second portion as a function of time is calculated from the stored measured images. A quantity of the fluorescently labeled molecules is calculated from the trace of the calculated intensity of the second portion as a function of time.

公开(公告)号：US20190299215A1

公开(公告)日：2019-10-03

申请号：US16302306

申请日：2017-05-24

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Andras Guttman ,  Marton Szigeti

IPC: B01L9/06 ,  B03C1/01 ,  B03C1/033 ,  B03C1/28

Abstract: A holder that can be used to stand up a PCR tube on a flat surface or held in a user's hand. The holder has a first elongated portion, a second elongated portion and a third elongated portion. The first elongated portion having a magnet, the second elongated portion having a tube holding means and the third elongate portion acting as a stabilizer to allow the holder to standup in an upright position. The third elongate portion can also be used as a gripping surface to be held between fingers of a hand.

公开(公告)号：US20220341940A1

公开(公告)日：2022-10-27

申请号：US17641224

申请日：2020-09-10

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Andras Guttman ,  Marton Szigeti

IPC: G01N33/68 ,  G01N33/58 ,  G01N1/44 ,  G01N33/543

Abstract: A sample preparation workflow to facilitate deep N-glycomics analysis of human serum by capillary electrophoresis with laser induced fluorescence (CE-LIF) detection accommodates the higher sample concentration requirement of electrospray ionization mass spectrometry connected to capillary electrophoresis (CE-ESI-MS). A temperature gradient denaturing protocol is applied on amine functionalized magnetic bead partitioned glycoproteins to avoid precipitation. This also results in the free sugar content of the serum being significantly decreased which allows PNGase F mediated release of the N-linked carbohydrates. The liberated oligosaccharides were tagged with aminopyrene-trisulfonate, utilizing a modified evaporative labeling protocol. This workflow provides appropriate amounts of material for example for use in CE-ESI-MS analysis in negative ionization mode.

公开(公告)号：US11161119B2

公开(公告)日：2021-11-02

申请号：US16302306

申请日：2017-05-24

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Andras Guttman ,  Marton Szigeti

IPC: B01L9/06 ,  B03C1/01 ,  G01N35/00 ,  B03C1/28 ,  B03C1/033

Abstract: A holder that can be used to stand up a PCR tube on a flat surface or held in a user's hand. The holder has a first elongated portion, a second elongated portion and a third elongated portion. The first elongated portion having a magnet, the second elongated portion having a tube holding means and the third elongate portion acting as a stabilizer to allow the holder to standup in an upright position. The third elongate portion can also be used as a gripping surface to be held between fingers of a hand.

公开(公告)号：US20190234867A1

公开(公告)日：2019-08-01

申请号：US16328208

申请日：2017-08-17

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Gabor Jarvas ,  Marton Geza Szigeti ,  Andras Guttman ,  Jeff Chapman

IPC: G01N21/33 ,  G01N33/53 ,  G16C20/20

CPC classification number: G01N21/33 ,  G01N21/6402 ,  G01N27/44726 ,  G01N33/5308 ,  G01N2030/045 ,  G01N2030/8836 ,  G16C20/20

Abstract: A separation device receives a known or unknown glycan that is co-injected with three different oligomers maltooligosaccharide (MOL). A detector measures the separated glycan and the separated three different oligomers as intensity peaks that are a function of migration time. The migration times of a plurality of other oligomers of MOL are calculated from the migration times of the three different oligomers. Glucose unit (GU) values for the intensity peaks of the separated glycan are calculated by comparing their migration times to the calculated migration times of the plurality of other oligomers of MOL. The processor identifies the structure of the glycan by comparing the calculated GU values of the intensity peaks of the separated glycan to a database of GU values for known glycan structures.