公开(公告)号：US20200291463A1

公开(公告)日：2020-09-17

申请号：US16809674

申请日：2020-03-05

Applicant: Duke University

Inventor： Tuan Vo-Dinh ,  Hsin-Neng Wang ,  Andrew Fales

IPC: C12Q1/6825 ,  A61K31/713

Abstract: Plasmonics-active nanoprobes are provided for detection of target biomolecules including nucleic acids, proteins, and small molecules. The nucleic acids that can be detected include RNA, DNA, mRNA, microRNA, and small nucleotide polymorphisms (SNPs). The nanoproprobes can be used in vito in sensitive detection methods for diagnosis of diseases and disorders including cancer. Multiplexing can be performed using the nanoprobes such that multiple targets can be detected simultaneously in a single sample. The methods of use of the nanoprobes include detection by a visible color change. The nanoprobes can be used in vivo for treatment of undesireable cells in a subject.

公开(公告)号：US20240401113A1

公开(公告)日：2024-12-05

申请号：US18679556

申请日：2024-05-31

Applicant: Duke University

Inventor： Tuan Vo-Dinh ,  Hsin-Neng Wang

IPC: C12Q1/6823 ,  C12Q1/6837

Abstract: To overcome the limitations of existing methods for detecting short nucleic acid molecules of low abundance such as miRNA, the present inventors provide a non-enzymatic signal amplification method based on inverse molecular sentinel (iMS) nanoprobes to improve detection sensitivity. The method is based on a cascade toehold-mediated DNA strand displacement reaction triggered by a “linear” DNA strand called “Recycling Trigger Probe” (RTP) strand. In the method, iMS-OFF nanoprobes are incubated with targets and RTP strands. After turning on the first nanoprobe, the target undergoes a recycling process triggered by the RTP strands. This process allows the target to turn on more iMS nanoprobes and provide an amplified SERS signal.

公开(公告)号：US10358680B2

公开(公告)日：2019-07-23

申请号：US15442731

申请日：2017-02-27

Applicant: Duke University

Inventor： Tuan Vo-Dinh ,  Hsin-Neng Wang

IPC: C07H21/04 ,  C12Q1/6886 ,  C12Q1/6816

Abstract: Plasmonics-active nanoprobes are provided for detection of target biomolecules including nucleic acids, proteins, and small molecules. The nucleic acids that can be detected include RNA, DNA, mRNA, microRNA, and small nucleotide polymorphisms (SNPs). The nanoproprobes can be used in vito in sensitive detection methods for diagnosis of diseases and disorders including cancer. Multiplexing can be performed using the nanoprobes such that multiple targets can be detected simultaneously in a single sample. The methods of use of the nanoprobes include detection by a visible color change. The nanoprobes can be used in vivo for treatment of undesireable cells in a subject.

公开(公告)号：US12173355B2

公开(公告)日：2024-12-24

申请号：US16809674

申请日：2020-03-05

Applicant: Duke University

Inventor： Tuan Vo-Dinh ,  Hsin-Neng Wang ,  Andrew Fales

IPC: C12Q1/68 ,  A61K31/713 ,  C12Q1/6825

Abstract: Plasmonics-active nanoprobes are provided for detection of target biomolecules including nucleic acids, proteins, and small molecules. The nucleic acids that can be detected include RNA, DNA, mRNA, microRNA, and small nucleotide polymorphisms (SNPs). The nanoproprobes can be used in vito in sensitive detection methods for diagnosis of diseases and disorders including cancer. Multiplexing can be performed using the nanoprobes such that multiple targets can be detected simultaneously in a single sample. The methods of use of the nanoprobes include detection by a visible color change. The nanoprobes can be used in vivo for treatment of undesireable cells in a subject.

公开(公告)号：US20170321280A1

公开(公告)日：2017-11-09

申请号：US15442731

申请日：2017-02-27

Applicant: Duke University

Inventor： Tuan Vo-Dinh ,  Hsin-Neng Wang

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q1/6816 ,  C12Q2563/155 ,  C12Q2565/101

Abstract: Plasmonics-active nanoprobes are provided for detection of target biomolecules including nucleic acids, proteins, and small molecules. The nucleic acids that can be detected include RNA, DNA, mRNA, microRNA, and small nucleotide polymorphisms (SNPs). The nanoproprobes can be used in vito in sensitive detection methods for diagnosis of diseases and disorders including cancer. Multiplexing can be performed using the nanoprobes such that multiple targets can be detected simultaneously in a single sample. The methods of use of the nanoprobes include detection by a visible color change. The nanoprobes can be used in vivo for treatment of undesireable cells in a subject.

公开(公告)号：US20160076086A1

公开(公告)日：2016-03-17

申请号：US14861353

申请日：2015-09-22

Applicant: DUKE UNIVERSITY

Inventor： Tuan Vo-Dinh ,  Hsin-Neng Wang ,  Andrew Fales

IPC: C12Q1/68 ,  A61K9/16 ,  A61K31/713

CPC classification number: C12Q1/6825 ,  A61K31/713 ,  C12Q2525/301 ,  C12Q2563/137 ,  C12Q2563/155

Abstract: Plasmonics-active nanoprobes are provided for detection of target biomolecules including nucleic acids, proteins, and small molecules. The nucleic acids that can be detected include RNA, DNA, mRNA, microRNA, and small nucleotide polymorphisms (SNPs). The nanoproprobes can be used in vito in sensitive detection methods for diagnosis of diseases and disorders including cancer. Multiplexing can be performed using the nanoprobes such that multiple targets can be detected simultaneously in a single sample. The methods of use of the nanoprobes include detection by a visible color change. The nanoprobes can be used in vivo for treatment of undesireable cells in a subject.

Abstract translation: 提供等离子体激活的纳米探针用于检测目标生物分子，包括核酸，蛋白质和小分子。 可检测的核酸包括RNA，DNA，mRNA，微小RNA和小核苷酸多态性（SNPs）。 纳米探针可用于敏感检测方法，用于诊断包括癌症在内的疾病和病症。 可以使用纳米探针进行复用，使得可以在单个样本中同时检测多个靶。 纳米探针的使用方法包括通过可见的颜色变化的检测。 纳米探针可用于体内治疗受试者中不需要的细胞。