公开(公告)号：US20190200892A1

公开(公告)日：2019-07-04

申请号：US16323705

申请日：2017-08-08

Applicant: Eccrine Systems, Inc.

Inventor： Robert Beech ,  Gavi Begtrup ,  Jason Heikenfeld ,  Jacob A Bertrand

IPC: A61B5/053 ,  A61B10/00 ,  A61B5/145 ,  A61B5/1468 ,  G01N27/327

CPC classification number: A61B5/053 ,  A61B5/14517 ,  A61B5/1468 ,  A61B5/6833 ,  A61B10/0064 ,  A61B2560/0412 ,  A61B2560/0462 ,  A61N1/30 ,  A61N1/32 ,  G01N27/3277

Abstract: The disclosed invention includes integrative electrochemical aptamer-based sensors for use in wearable biofluid sensing devices. The disclosed integrative EAB sensors are configured to detect very low concentrations of target analytes in a sweat or biofluid sample by aggregating signals from individual sensing elements over time until a signal threshold is reached. Signal aggregation is accomplished through various retention structures that extend the time sensing elements retain target analyte molecules. Embodiments include attaching complementary primers and functional groups to the aptamer, covering such retention structures with blockers until analyte capture, or coating the sensor electrode with a hydrophilic and hydrophobic monolayer. The invention also includes methods of using the disclosed integrative sensors. Some embodiments of the disclosed method include tracking time to signal threshold to estimate analyte concentration.

公开(公告)号：US20210140956A1

公开(公告)日：2021-05-13

申请号：US16605145

申请日：2018-11-16

Applicant: Eccrine Systems, Inc.

Inventor： Mikel Larson ,  Leila Safazadeh Haghighi ,  Jacob A Bertrand ,  Florika Macazo

IPC: G01N33/543 ,  C12N15/11

Abstract: Electrochemical aptamer-based (EAB) biosensing devices are described that provide drift correction and calibration to EAB sensor measurements of biofluid analyte concentrations by disclosing reference sensors that are configured to not interact with a target analyte, but otherwise mirror the performance of active EAB sensors within the expected application parameters of the device. Such reference sensors are configured to allow comparisons with their companion active sensors to track aptamer sensing element dissociation from an electrode surface, temperature-induced effects, redox moiety dissociation, and/or the effects of surface fouling. Some embodiments include separate electrodes for active and reference aptamer sensing elements. Other embodiments include a single electrode for both active and reference aptamer sensing elements. Single electrode embodiments include two or more distinct redox moieties.

公开(公告)号：US20210285936A1

公开(公告)日：2021-09-16

申请号：US16624062

申请日：2018-06-25

Applicant: Eccrine Systems, Inc.

Inventor： Robert Beech ,  Mikel Larson ,  Jacob A Bertrand ,  Gavi Begtrup

IPC: G01N33/53 ,  G01N33/543 ,  G01N27/327

Abstract: Electrochemical aptamer-based biosensing devices and methods are described herein that are configured to produce a detectible signal upon target analyte interaction, with reduced reliance on a conformational change by the aptamer. The disclosure includes embodiments of docked aptamer EAB sensors for measuring the presence of a target analyte in a biofluid sample. The sensors include an electrode capable of sensing redox events, and a plurality of aptamer sensing elements with aptamers selected to interact with a target analyte. Each aptamer sensing element includes a molecular docking structure attached to the electrode, and an analyte capture complex that includes an aptamer releasably bound to the docking structure, and an electroactive redox moiety. Upon the aptamer binding with a target analyte, the analyte capture complex separates from the docking structure. The separation of the analyte capture complex from the docking structure produces a positional change in the redox moiety that is detectable by the sensing device on interrogation of the electrode.

公开(公告)号：US20210282671A1

公开(公告)日：2021-09-16

申请号：US16605979

申请日：2019-03-22

Applicant: Eccrine Systems, Inc.

Inventor： Michelle Hoffman ,  Jason Heikenfeld ,  Jacob A Bertrand ,  Nicholas Bailey

IPC: A61B5/145 ,  A61B10/00 ,  A61B5/01

Abstract: Embodiments of the disclosed invention provide wearable devices that use a humidity sensor to measure sweat rate generated from an area of skin. A sensing chamber is continuously filled with a sweat sample, which forms a droplet and alters the humidity measured within the chamber. Once the sweat sample droplet expands to the edge of the chamber, the droplet contacts a wick and is drawn away, so the chamber can fill with a subsequent droplet. The device uses a droplet volume and the time required to reach a maximum humidity to calculate a sweat rate. A pump is used to draw old sweat sample out of the wick to allow extended device operation. Some embodiments also include capacitive sensors to perform back up measurements. Another set of embodiments includes alternatively shaped sensing chambers configured to reduce sample volumes or improve function. A method for determining sweat rate based on humidity sensor measurements is also included.