公开(公告)号：US20190142311A1

公开(公告)日：2019-05-16

申请号：US16163011

申请日：2018-10-17

Applicant: Eccrine Systems, Inc.

Inventor： Jason C. Heikenfeld ,  Mikel Larson ,  Florika Macazo ,  Jacob A. Bertrand

IPC: A61B5/145 ,  A61B5/1495 ,  A61B5/00

Abstract: Devices for calibrating electrochemical aptamer-based biofluid sensors. In some embodiments a calibrant is located proximate to the EAB sensors, and calibration is facilitated by introduction of the biofluid into the device. Other embodiments include various mechanisms for introducing calibration solution to the sensors, including drawing calibration solution into contact with sensors through electrical potential, a dispensing mechanism such as an ink jet-type nozzle, as well as pressure actuated calibration dispensing. Also included are embodiments employing bifurcated biofluid transport paths that allow alternately for calibration and biofluid sensing.

公开(公告)号：US20210285936A1

公开(公告)日：2021-09-16

申请号：US16624062

申请日：2018-06-25

Applicant: Eccrine Systems, Inc.

Inventor： Robert Beech ,  Mikel Larson ,  Jacob A Bertrand ,  Gavi Begtrup

IPC: G01N33/53 ,  G01N33/543 ,  G01N27/327

Abstract: Electrochemical aptamer-based biosensing devices and methods are described herein that are configured to produce a detectible signal upon target analyte interaction, with reduced reliance on a conformational change by the aptamer. The disclosure includes embodiments of docked aptamer EAB sensors for measuring the presence of a target analyte in a biofluid sample. The sensors include an electrode capable of sensing redox events, and a plurality of aptamer sensing elements with aptamers selected to interact with a target analyte. Each aptamer sensing element includes a molecular docking structure attached to the electrode, and an analyte capture complex that includes an aptamer releasably bound to the docking structure, and an electroactive redox moiety. Upon the aptamer binding with a target analyte, the analyte capture complex separates from the docking structure. The separation of the analyte capture complex from the docking structure produces a positional change in the redox moiety that is detectable by the sensing device on interrogation of the electrode.

公开(公告)号：US20200298231A1

公开(公告)日：2020-09-24

申请号：US16649211

申请日：2018-09-21

Applicant: University Of Cincinnati ,  Eccrine Systems, Inc.

Inventor： Jessica Francis ,  Mikel Larson ,  Michelle Hoffman ,  Eliot Gomez ,  Jason Charles Heikenfeld ,  Isaac Stamper

IPC: B01L3/00 ,  A61B5/145

Abstract: A device for determining the amount or concentration of an analyte in a fluid sample and a flow rate of the fluid sample in a channel is provided. The device includes a chamber including a channel and an opening the channel in fluid communication with the opening. The device further includes a wicking component positioned adjacent to the opening configured to receive an amount of fluid from the channel. The device may further include an analyte sensor positioned on the wicking component, the analyte sensor configured to detect an analyte in fluid in contact with the analyte sensor, wherein the wicking component is configured to contact the amount of fluid with the analyte sensor. Alternatively the device may include at least one pair of electrodes configured to determine a flow rate of the fluid in the channel.

公开(公告)号：US11524290B2

公开(公告)日：2022-12-13

申请号：US16649211

申请日：2018-09-21

Applicant: University Of Cincinnati ,  Eccrine Systems, Inc.

Inventor： Jessica Francis ,  Mikel Larson ,  Michelle D. Hoffman ,  Eliot Gomez ,  Jason Charles Heikenfeld ,  Isaac Stamper

IPC: B01L3/00 ,  A61B5/145

Abstract: A device for determining the amount or concentration of an analyte in a fluid sample and a flow rate of the fluid sample in a channel is provided. The device includes a chamber including a channel and an opening the channel in fluid communication with the opening. The device further includes a wicking component positioned adjacent to the opening configured to receive an amount of fluid from the channel. The device may further include an analyte sensor positioned on the wicking component, the analyte sensor configured to detect an analyte in fluid in contact with the analyte sensor, wherein the wicking component is configured to contact the amount of fluid with the analyte sensor. Alternatively the device may include at least one pair of electrodes configured to determine a flow rate of the fluid in the channel.

公开(公告)号：US20210140956A1

公开(公告)日：2021-05-13

申请号：US16605145

申请日：2018-11-16

Applicant: Eccrine Systems, Inc.

Inventor： Mikel Larson ,  Leila Safazadeh Haghighi ,  Jacob A Bertrand ,  Florika Macazo

IPC: G01N33/543 ,  C12N15/11

Abstract: Electrochemical aptamer-based (EAB) biosensing devices are described that provide drift correction and calibration to EAB sensor measurements of biofluid analyte concentrations by disclosing reference sensors that are configured to not interact with a target analyte, but otherwise mirror the performance of active EAB sensors within the expected application parameters of the device. Such reference sensors are configured to allow comparisons with their companion active sensors to track aptamer sensing element dissociation from an electrode surface, temperature-induced effects, redox moiety dissociation, and/or the effects of surface fouling. Some embodiments include separate electrodes for active and reference aptamer sensing elements. Other embodiments include a single electrode for both active and reference aptamer sensing elements. Single electrode embodiments include two or more distinct redox moieties.

公开(公告)号：US10736565B2

公开(公告)日：2020-08-11

申请号：US15754141

申请日：2017-10-16

Applicant: Eccrine Systems, Inc.

Inventor： Gavi Begtrup ,  Austin Morgan ,  Mikel Larson ,  Jacob A. Bertrand ,  Nicholas Bailey ,  Cory Newland ,  Robert Beech ,  Brian Hanley

IPC: A61B5/00 ,  A61B5/145 ,  A61B5/1477 ,  A61B5/0205 ,  A61B5/20 ,  G01N33/487 ,  A61B5/11 ,  A61B5/024 ,  A61B5/053 ,  A61B5/01

Abstract: Embodiments of the disclosed invention provide devices and methods to incorporate suspension-based, i.e., hydrogel-based and thixotropic compound-based, ion-selective electrodes and reference electrodes into a wearable sweat sensing device. Embodiments of this device are configured to monitor sweat electrolyte concentrations, trends, and ratios under demanding use conditions. The accompanying method includes use of the disclosed device to track fluid and electrolyte gain and loss in order to produce an electrolyte estimate, such as a sweat electrolyte concentration, a sweat electrolyte concentration trend, a sweat rate, or a concentration ratio between a plurality of electrolytes.

公开(公告)号：US20200155048A1

公开(公告)日：2020-05-21

申请号：US16623713

申请日：2018-06-20

Applicant: Eccrine Systems, Inc.

Inventor： Jacob A Bertand ,  Brian Hanley ,  Mikel Larson ,  Gavi Begtrup

IPC: A61B5/145 ,  G01N1/28 ,  A61B5/1477

Abstract: Devices and methods for tuning biofluid sample pH to enable more accurate analyte concentration measurements with pH-sensitive biosensors. In the embodiments, biofluid samples react with a polymer buffering material during transfer to a sensing element. The reaction with the buffering material causes protonation or deprotonation of the sample based upon 1) the pH of the sample, and 2) the selected quantity and pKa of the functional groups in the buffering material. Controlling the H+ content of a biofluid sample has beneficial effects on the accuracy of the biosensor by reducing or eliminating signal changes due to redox moiety variability, thereby isolating signal changes reflecting analyte concentration.

公开(公告)号：US20190183398A1

公开(公告)日：2019-06-20

申请号：US16326317

申请日：2017-08-21

Applicant: Eccrine Systems, Inc.

Inventor： Jason Heikenfeld ,  Mikel Larson ,  Nathan Weinle

IPC: A61B5/145 ,  A61B5/01 ,  A61B5/00

CPC classification number: A61B5/14546 ,  A61B5/01 ,  A61B5/14503 ,  A61B5/1451 ,  A61B5/14514 ,  A61B5/14517 ,  A61B5/1455 ,  A61B5/14865 ,  A61B5/4272 ,  A61B5/4277 ,  A61B2010/0067 ,  A61B2560/0228 ,  A61B2560/0252 ,  A61B2562/0271 ,  A61B2562/028 ,  A61B2562/162

Abstract: The disclosed invention includes a biofluid sensing device capable of passively or actively regulating an operating temperature of one or more sensors. The device includes at least one biofluid sensor in a thermally isolated environment and at least one temperature sensor to measure sensor environment temperature. Some embodiments include at least one thermal adjustor to regulate the sensor temperature by actively regulating the sensor environment temperature in response to a signal from the temperature sensor. The invention also includes a method of regulating temperature for a biofluid sensing device having a sensor for measuring an analyte in the biofluid. The method includes measuring a biofluid sensor temperature, regulating the sensor temperature to within a selected range of the measured sensor temperature, and maintaining sensor temperature within the selected range of the measured temperature throughout device operation. In some embodiments, the measured temperature is a calibration temperature.

公开(公告)号：US20190008448A1

公开(公告)日：2019-01-10

申请号：US15754141

申请日：2017-10-16

Applicant: Eccrine Systems, Inc.

Inventor： Gavi Begtrup ,  Austin Morgan ,  Mikel Larson ,  Jacob A. Bertrand ,  Nicholas Bailey ,  Cory Newland ,  Robert Beech ,  Brian Hanley

IPC: A61B5/00 ,  A61B5/145 ,  A61B5/1477 ,  A61B5/0205 ,  A61B5/20 ,  G01N33/487

CPC classification number: A61B5/486 ,  A61B5/01 ,  A61B5/02055 ,  A61B5/02438 ,  A61B5/0533 ,  A61B5/0537 ,  A61B5/1118 ,  A61B5/14517 ,  A61B5/14546 ,  A61B5/1477 ,  A61B5/201 ,  A61B5/4266 ,  A61B5/4872 ,  A61B5/4875 ,  A61B5/6801 ,  A61B5/6833 ,  A61B5/7405 ,  A61B5/7435 ,  A61B5/7455 ,  A61B5/7475 ,  A61B2560/0242 ,  G01N33/48707 ,  G01N33/48792

Abstract: Embodiments of the disclosed invention provide devices and methods to incorporate suspension-based, i.e., hydrogel-based and thixotropic compound-based, ion-selective electrodes and reference electrodes into a wearable sweat sensing device. Embodiments of this device are configured to monitor sweat electrolyte concentrations, trends, and ratios under demanding use conditions. The accompanying method includes use of the disclosed device to track fluid and electrolyte gain and loss in order to produce an electrolyte estimate, such as a sweat electrolyte concentration, a sweat electrolyte concentration trend, a sweat rate, or a concentration ratio between a plurality of electrolytes.