公开(公告)号：US09752199B2

公开(公告)日：2017-09-05

申请号：US15086769

申请日：2016-03-31

Applicant: FUNDAMENTAL SOLUTIONS CORPORATION

Inventor： Thomas J. Zupancic ,  Lingchun Zeng ,  Srikanth Vedamoorthy ,  Joel S. Lwande ,  Joesph D. Kittle ,  Min Mo

IPC: C07K16/12 ,  C12Q1/68 ,  C07K14/315 ,  C07K14/725 ,  C07K14/735 ,  C07K14/36 ,  G01N33/536 ,  G01N33/569 ,  G01N33/50

CPC classification number: C12Q1/6897 ,  C07K14/315 ,  C07K14/36 ,  C07K14/7051 ,  C07K14/70535 ,  C07K16/12 ,  C07K2317/52 ,  C07K2319/22 ,  C07K2319/60 ,  G01N33/5047 ,  G01N33/536 ,  G01N33/56916 ,  G01N2333/245

Abstract: A system, device, and method for rapid detection of analytes that includes a living, engineered biosensor cell that is typically a component of the mammalian immune system; a reporter protein that is engineered into and expressed by the living, engineered biosensor cell, wherein the reporter protein emits a detectable signal in response to certain predetermined changes in the cytosol of the living, engineered cell; a signal transduction pathway expressed by the living, engineered biosensor cell, wherein the signal transduction pathway controls a biological process within the cytosol of the living, engineered biosensor cell, and wherein the biochemical process, when it occurs, causes the reporter protein to emit a detectable signal; at least one type of detector molecule that is adapted to bind to a specific analyte; at least one analyte that binds to the detector molecule that is specific to that analyte; a plurality of non-antibody signal transducing elements that are either expressed by the living, engineered biosensor cell or that actively bind to a receptor or a receptor component expressed by the living, engineered biosensor cell, wherein each signal transducing element is adapted to receive a detector molecule.