公开(公告)号：US20140353524A1

公开(公告)日：2014-12-04

申请号：US14461779

申请日：2014-08-18

Applicant: GE HEALTCARE BIO-SCIENCES CORP.

Inventor： GAUDENZ DANUSER ,  PAUL C. GOODWIN

IPC: G01N21/64

CPC classification number: G01N21/6428 ,  G01N21/636 ,  G01N21/6458 ,  G01N2021/6415 ,  G01N2021/6419 ,  G01N2201/126 ,  G02B21/365 ,  G02B21/367 ,  G06T3/4053

Abstract: Embodiments of the present invention are directed to imaging technologies, and, in particular, to an imaging system that detects relatively weak signals, over time, and that uses the detected signals to determine the positions of signal emitters. Particular embodiments of the present invention are directed to methods and systems for imaging fluorophore-labeled samples in order to produce images of the sample at resolutions significantly greater than the diffraction-limited resolution associated with optical microscopy. Embodiments of the present invention employ overlapping-emitter-image disambiguation to allow data to be collected from densely arranged emitters, which significantly decreases the data-collection time for producing intermediate images as well as the number of intermediate images needed to computationally construct high-resolution final images. Additional embodiments of the present invention employ hierarchical image-processing techniques to further resolve and interpret disambiguated images.

Abstract translation: 本发明的实施例涉及成像技术，特别涉及一种随时间检测相对较弱信号的成像系统，并且使用所检测的信号来确定信号发射器的位置。 本发明的具体实施方案涉及用于成像荧光团标记的样品的方法和系统，以便以明显大于与光学显微镜相关联的衍射限制分辨率的分辨率产生样品的图像。 本发明的实施例采用重叠 - 发射 - 图像消歧以允许从密集布置的发射器收集数据，这显着减少用于产生中间图像的数据收集时间以及计算构建高分辨率所需的中间图像的数量 最终图像。 本发明的另外的实施例采用分层图像处理技术来进一步解析和解释消歧图像。