公开(公告)号：US20210322336A1

公开(公告)日：2021-10-21

申请号：US17292455

申请日：2019-12-16

Applicant: HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Jiangling Wan ,  Han LUO ,  Jianyong SHENG ,  Xiangliang YANG

IPC: A61K9/51 ,  A61K31/337 ,  A61K47/42 ,  A61P35/00

Abstract: A method for preparing albumin nanoparticles based on free radical oxidation, comprising the following steps: preparing albumin aqueous solution with an albumin concentration of 1-20 mg/mL; (2) whilst stirring, adding free radical generating agent to the albumin aqueous solution, and continuing to stir and react for 1-60 min at 15-50° C.; the free radicals generated by the free radical generating agent are used for oxidizing the albumin, and the oxidized albumin molecules assemble to form albumin nanoparticles that are mainly bonded by hydrophobic action, thereby obtaining an albumin nanoparticle suspension. The present invention implements improvement by means of the key reaction mechanism of the nanoparticle preparation method and the corresponding design of the finishing process and, compared to the prior art, can effectively solve the problems of the complexity, high cost, and high toxicity of preparing nanoparticles.

公开(公告)号：US11318105B2

公开(公告)日：2022-05-03

申请号：US17292455

申请日：2019-12-16

Applicant: HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Jiangling Wan ,  Han Luo ,  Jianyong Sheng ,  Xiangliang Yang

IPC: A61K9/51 ,  A61K31/337 ,  A61K47/42 ,  A61P35/00

Abstract: A method for preparing albumin nanoparticles based on free radical oxidation, comprising the following steps: preparing albumin aqueous solution with an albumin concentration of 1-20 mg/mL; (2) whilst stirring, adding free radical generating agent to the albumin aqueous solution, and continuing to stir and react for 1-60 min at 15-50° C.; the free radicals generated by the free radical generating agent are used for oxidizing the albumin, and the oxidized albumin molecules assemble to form albumin nanoparticles that are mainly bonded by hydrophobic action, thereby obtaining an albumin nanoparticle suspension. The present invention implements improvement by means of the key reaction mechanism of the nanoparticle preparation method and the corresponding design of the finishing process and, compared to the prior art, can effectively solve the problems of the complexity, high cost, and high toxicity of preparing nanoparticles.