公开(公告)号：US08525985B2

公开(公告)日：2013-09-03

申请号：US13658872

申请日：2012-10-24

Applicant: Hitachi, Ltd.

Inventor： Toru Fujimura ,  Kosuke Kuwabara ,  Tatsuro Ide

IPC: G01N21/00 ,  G01N30/74

CPC classification number: G01N21/05 ,  G01N21/31 ,  G01N21/55 ,  G01N33/54373 ,  G01N2021/0346 ,  G01N2201/061 ,  G01N2201/08

Abstract: The invention makes it possible to measure binding of a biochemical substance with a high throughput and with high sensitivity using a small cell capable of being filled with a small amount of chemical solution. A space between a first substrate and a second substrate such that probes are immobilized on their mutually facing planes is used as a cell that houses a specimen solution. Light is irradiated from a first substrate side, and reflected light is subjected to spectroscopy. Binding of the target with the probe is detected by a wavelength shift in the refection spectrum.

Abstract translation: 本发明能够使用能够填充少量化学溶液的小细胞来测量生物化学物质与高产量和高灵敏度的结合。 使用第一基板和第二基板之间的空间，使得将探针固定在其相互面对的平面上，作为容纳样本溶液的单元。 从第一基板侧照射光，反射光进行光谱分析。 通过反射光谱中的波长偏移来检测目标与探针的结合。

公开(公告)号：US08922765B2

公开(公告)日：2014-12-30

申请号：US13961173

申请日：2013-08-07

Applicant: Hitachi, Ltd.

Inventor： Toru Fujimura ,  Kosuke Kuwabara ,  Tatsuro Ide

IPC: G01N21/00 ,  G01N21/55 ,  G01N21/05 ,  G01N21/31 ,  G01N21/03

CPC classification number: G01N21/05 ,  G01N21/31 ,  G01N21/55 ,  G01N33/54373 ,  G01N2021/0346 ,  G01N2201/061 ,  G01N2201/08

Abstract: The invention makes it possible to measure binding of a biochemical substance with a high throughput and with high sensitivity using a small cell capable of being filled with a small amount of chemical solution. A space between a first substrate and a second substrate such that probes are immobilized on their mutually facing planes is used as a cell that houses a specimen solution. Light is irradiated from a first substrate side, and reflected light is subjected to spectroscopy. Binding of the target with the probe is detected by a wavelength shift in the refection spectrum.

Abstract translation: 本发明能够使用能够填充少量化学溶液的小细胞来测量生物化学物质与高产量和高灵敏度的结合。 使用第一基板和第二基板之间的空间，使得将探针固定在其相互面对的平面上，作为容纳样本溶液的单元。 从第一基板侧照射光，反射光进行光谱分析。 通过反射光谱中的波长偏移来检测靶与探针的结合。

公开(公告)号：US09291548B2

公开(公告)日：2016-03-22

申请号：US14558102

申请日：2014-12-02

Applicant: Hitachi, Ltd.

Inventor： Toru Fujimura ,  Kosuke Kuwabara ,  Tatsuro Ide

IPC: G01N21/05 ,  G01N33/543 ,  G01N21/55 ,  G01N21/31 ,  G01N21/03

CPC classification number: G01N21/05 ,  G01N21/31 ,  G01N21/55 ,  G01N33/54373 ,  G01N2021/0346 ,  G01N2201/061 ,  G01N2201/08

Abstract: The invention makes it possible to measure binding of a biochemical substance with a high throughput and with high sensitivity using a small cell capable of being filled with a small amount of chemical solution. A space between a first substrate and a second substrate such that probes are immobilized on their mutually facing planes is used as a cell that houses a specimen solution. Light is irradiated from a first substrate side, and reflected light is subjected to spectroscopy. Binding of the target with the probe is detected by a wavelength shift in the refection spectrum.