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    CYTOMETRIC MECHANISM, CELL CULTURE DEVICE COMPRISING SAME, AND CYTOMETRIC METHOD
    2.
    发明申请
    CYTOMETRIC MECHANISM, CELL CULTURE DEVICE COMPRISING SAME, AND CYTOMETRIC METHOD 审中-公开

    公开(公告)号：US20170306287A1

    公开(公告)日：2017-10-26

    申请号：US15518041

    申请日：2014-10-22

    Applicant: Hitachi High-Technologies Corporation

    Inventor： Masako KAWARAI ,  Toshinari SAKURAI ,  Akihiro SHIMASE ,  Hiroyuki KOSHI

    IPC: C12M1/34 ,  C12M1/00 ,  C12M1/36 ,  G01N21/53 ,  G01N33/483 ,  G01N15/06 ,  G01N15/00

    Abstract: A cytometric mechanism includes: a flow path through which a cell suspension is made to flow; a liquid drive unit for sending the cell suspension which is in the flow path; and a computation unit for irradiating, with irradiation light from a light source, a cell suspension flowing through a flow cell, and for finding a cell survival rate in the cell suspension on the basis of a resulting forward scattered light intensity and transmittance and/or side scattered light intensity. The invention is provided with a calibration curve database for storing, in advance, respective calibration curves indicative of a relationship between viable cell concentration and forward scattered light intensity, a relationship between dead cell concentration and the transmittance, and a relationship between a cell survival rate and the side scattered light intensity.

    CELL CONCENTRATION ADJUSTMENT DEVICE, AND AUTOMATIC SUBCULTURE SYSTEM USING SAME
    3.
    发明申请
    CELL CONCENTRATION ADJUSTMENT DEVICE, AND AUTOMATIC SUBCULTURE SYSTEM USING SAME 审中-公开

    公开(公告)号：US20170159003A1

    公开(公告)日：2017-06-08

    申请号：US15327208

    申请日：2015-07-08

    Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

    Inventor： Akihiro SHIMASE ,  Kazumichi IMAI ,  Sadamitsu ASO ,  Eiichiro TAKADA ,  Masako KAWARAI ,  Toshinari SAKURAI

    IPC: C12M1/34 ,  C12M1/02 ,  C12M1/36 ,  C12M1/00

    CPC classification number: C12M41/40 ,  C12M23/58 ,  C12M27/00 ,  C12M29/14 ,  C12M29/18 ,  C12M31/10 ,  C12M41/06 ,  C12M41/36 ,  C12M41/48 ,  C12Q1/06

    Abstract: A device which automatically performs a step in which expanded and cultured cells are diluted to a desired cell concentration and re-inoculated using a cell-concentration adjustment device having an inlet for taking in a cell suspension; an outlet for discharging a diluted cell suspension; and a flow path which is provided between the inlet and the outlet and is capable of holding a cell suspension, the flow path being provided with: a liquid delivery pump for causing a cell suspension inside to flow; a cell-concentration measurement instrument for collecting data related to a cell concentration per unit amount of the cell suspension; and a dilution-liquid container for holding a dilution liquid which is supplied to the flow path to dilute the cell suspension. The device further includes a control unit for controlling at least the liquid delivery pump on the basis of the data obtained by the cell-concentration measurement instrument, wherein the control unit determines, on the basis of the data obtained by the cell-concentration measurement instrument, an amount of the dilution liquid required to bring the cell concentration to the desired concentration, and drives the liquid delivery pump so as to take in the required amount of the dilution liquid into the flow path and mix the cell suspension and the dilution liquid.

    STERILE CONNECTOR AND CELL CULTURE DEVICE PROVIDED THEREWITH
    4.
    发明申请
    STERILE CONNECTOR AND CELL CULTURE DEVICE PROVIDED THEREWITH 审中-公开

    公开(公告)号：US20190153377A1

    公开(公告)日：2019-05-23

    申请号：US15521348

    申请日：2014-11-07

    Applicant: Hitachi High-Technologies Corporation

    Inventor： Akihiro SHIMASE ,  Eiichiro TAKADA ,  Kazumichi IMAI ,  Toshinari SAKURAI

    IPC: C12M1/12 ,  C12M1/00 ,  C12M3/00

    Abstract: A sterile connector has a first connector including a first housing provided with a first flow channel; a first pipeline connected to the first flow channel; first and second openings, the first opening being positioned inward from the second opening; and a first sealing member covering the second opening. Also, a second connector includes a second housing provided with a second flow channel; a third opening; and a second sealing member covering the third opening. The first and second connectors are detachable from each other. The first sealing member seals a space between the inner circumferential surface of the first housing and the outer circumferential surface of the second housing, while the second sealing member seals a space between the inner circumferential surface of the second housing and the outer circumferential surface of the first pipeline so that the first flow channel communicates with the second flow channel.

    FLOW PASSAGE MODULE AND CELL CULTURE APPARATUS USING SAME
    6.
    发明申请
    FLOW PASSAGE MODULE AND CELL CULTURE APPARATUS USING SAME 审中-公开

    公开(公告)号：US20180258377A1

    公开(公告)日：2018-09-13

    申请号：US15761114

    申请日：2015-09-28

    Applicant: Hitachi High-Technologies Corporation

    Inventor： Akihiro SHIMASE ,  Toshinari SAKURAI

    IPC: C12M1/00 ,  F16K7/06 ,  B81B3/00 ,  B01F3/08 ,  F04B45/06

    CPC classification number: C12M1/007 ,  B01F3/08 ,  B01F5/102 ,  B01F15/0245 ,  B81B3/0097 ,  C12M23/40 ,  F04B45/06 ,  F16K7/06 ,  F16K11/022

    Abstract: Provided are a flow passage module which can achieve complete liquid substitution in a circulating flow passage with a simple structure, and a cell culture apparatus using said flow passage module. A flow passage module comprises: a flexible branching section which is provided with a first branching flow passage connected with an inflow passage for a fluid, a second branching flow passage connected with an outflow passage, a third branching flow passage connected with an entry-side end part of a circulating flow passage, and a fourth branching flow passage connected with an exit-side end part of the circulating flow passage, and which enables the branching flow passages to be communicated with each other; and a communication state switching part which has opening/closing members for closing and opening the desired branching flow passage from among the plurality of branching flow passages.

    CELL DISPERSION DEVICE, AND AUTOMATIC SUBCULTURE SYSTEM USING SAME
    7.
    发明申请
    CELL DISPERSION DEVICE, AND AUTOMATIC SUBCULTURE SYSTEM USING SAME 审中-公开

    公开(公告)号：US20180080002A1

    公开(公告)日：2018-03-22

    申请号：US15326938

    申请日：2015-07-08

    Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

    Inventor： Akihiro SHIMASE ,  Kazumichi IMAI ,  Sadamitsu ASO ,  Eiichiro TAKADA ,  Masako KAWARAI ,  Toshinari SAKURAI

    IPC: C12M1/36 ,  C12M1/00 ,  C12M1/34 ,  C12M1/33

    CPC classification number: C12M41/48 ,  C12M1/00 ,  C12M1/02 ,  C12M1/34 ,  C12M29/00 ,  C12M41/00 ,  C12M41/36 ,  C12M45/02 ,  C12M47/04

    Abstract: The purpose of the present invention is to provide a means for dispersing cell aggregates without damaging the cells, such that a sufficient multiplication rate can be obtained in a subculture. According to the present invention, provided is a cell-suspension processing device which disperses cell aggregates included in a cell suspension. The device is provided with: an inlet for taking in the cell suspension; an outlet for discharging the processed cell suspension; and a flow path which is provided between the inlet and the outlet, and which is capable of holding the cell suspension. The flow path has, provided thereto, a liquid delivery pump for causing the cell suspension inside to flow, a cell-dispersion-degree measurement instrument for measuring the dispersion degree of cells in the cell suspension, and a narrow part for imparting shearing force to the cell suspension flowing inside. The cell-suspension processing device is further provided with a control unit for controlling at least the liquid delivery pump on the basis of data obtained by the cell-dispersion-degree measurement instrument. The control unit determines whether the cells have attained a prescribed dispersion degree on the basis of the data obtained by the cell-dispersion-degree measurement instrument, and, in cases when the cells have not attained the prescribed dispersion degree, drives the liquid delivery pump such that the cell suspension is passed through the narrow part.

    CELL CULTURE DEVICE
    8.
    发明申请
    CELL CULTURE DEVICE 审中-公开
    细胞培养装置

    公开(公告)号：US20170037351A1

    公开(公告)日：2017-02-09

    申请号：US15106945

    申请日：2014-12-24

    Applicant: Hitachi High-Technologies Corporation

    Inventor： Akihiro SHIMASE ,  Kazumichi IMAI ,  Eiichiro TAKADA ,  Sadamitsu ASO

    IPC: C12M1/00

    CPC classification number: C12M23/40 ,  C12M23/50 ,  C12M27/16 ,  C12M29/00

    Abstract: According to the present invention, a simple structure can be used to achieve reliable liquid delivery with no residual air, and simple attachment/detachment of a culture vessel, and thus a closed-system cell culture device exhibiting high reliability can be constructed at low cost. In the present invention, a liquid is supplied or discharged while a culture vessel is in an inclined state. The culture vessel is provided with two flow paths, namely an intake flow path and a discharge flow path, which connect a culture chamber and a connection member. Points where the flow paths join with the culture chamber are respectively configured as an intake port and a discharge port. The discharge port is provided in the position nearest to the axis of inclination of the culture vessel. The intake port is provided in a plane projected from a vertical plane including the aforementioned axis of inclination.

    Abstract translation: 根据本发明，可以使用简单的结构来实现无残留空气的可靠的液体输送，以及培养容器的简单的附着/分离，从而可以以低成本构建出具有高可靠性的封闭系统的细胞培养装置 。 在本发明中，在培养容器处于倾斜状态时供给或排出液体。 培养容器具有连接培养室和连接部件的两个流路，即进气流路和排出流路。 流路与培养室连接的点分别构成进气口和排出口。 排出口设置在最靠近培养容器倾斜轴线的位置。 进气口设置在从包括上述倾斜轴线的垂直平面投影的平面中。

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