公开(公告)号：US12146873B2

公开(公告)日：2024-11-19

申请号：US17849905

申请日：2022-06-27

Applicant: IMEC VZW

Inventor： Anne Verhulst ,  Pol Van Dorpe

IPC: G01N15/06 ,  G01N15/01 ,  G01N33/487

Abstract: A method of operating a pore field-effect transistor (FET) sensor for detecting particles, wherein the pore FET sensor comprises a FET wherein a gate is controlled by a pore filled by a fluid, comprises: controlling a first voltage (Vcis) to set the FET in a subthreshold region; controlling a second voltage (Vtrans) to set a voltage difference between the first and second voltages (Vtrans) such that an effective difference in gate voltage experienced between a minimum and a maximum effective gate voltage during movement of a particle in the fluid is at least kT/q; and detecting a drain-source current in the FET, wherein the particle passing through the pore modulates the drain-source current for detecting presence of the particle.

公开(公告)号：US11366060B2

公开(公告)日：2022-06-21

申请号：US16647011

申请日：2018-09-18

Applicant: IMEC VZW ,  Katholieke Universiteit Leuven

Inventor： Finub James Shirley ,  Pol Van Dorpe

IPC: G01N21/64

Abstract: An apparatus for detecting fluorescent light emitted from a sample comprises: a light source, which is configured to emit excitation light of an excitation wavelength towards a sample comprising fluorophores such that fluorescent light is induced; a photo-detector for detecting light incident on the photo-detector; and an interference filter arranged on the photo-detector, wherein the interference filter is configured to selectively collect and transmit light towards the photo-detector based on an angle of incidence of the light towards the interference filter, wherein the interference filter is configured to selectively transmit supercritical angle fluorescence from the sample towards the photo-detector and suppress undercritical angle fluorescence from the sample.

公开(公告)号：US10883939B2

公开(公告)日：2021-01-05

申请号：US16442484

申请日：2019-06-15

Applicant: IMEC VZW

Inventor： Pol Van Dorpe ,  Niels Verellen

IPC: G01N21/64 ,  G01N15/02 ,  G01N21/77

Abstract: An imaging apparatus comprises: (i) an illumination waveguide configured to propagate light by total internal reflection, wherein an evanescent field illuminates an object in close relation to the illumination waveguide; (ii) an array of light-sensitive areas arranged on a common substrate with the illumination waveguide for detecting light from the object; and (iii) a controller configured to control forming of an interference pattern in the illumination waveguide, wherein the interference pattern comprises at least one element of constructive interference for selectively illuminating a portion of the object, the at least one element having a dimension with a size in a range of 100 nm-10 μm; wherein the controller is configured to sequentially change the interference pattern in relation to the object such that different portions are illuminated and light from different portions is sequentially detected.

公开(公告)号：US20180172588A1

公开(公告)日：2018-06-21

申请号：US15736457

申请日：2016-06-30

Applicant: IMEC VZW

Inventor： Peter Peumans ,  Liesbet Lagae ,  Willem Van Roy ,  Tim Stakenborg ,  Pol Van Dorpe

IPC: G01N21/64

CPC classification number: G01N21/6408 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/648 ,  G01N2021/6413 ,  G01N2021/6439

Abstract: Sensor devices for quantifying luminescent targets are described herein. An example device comprises a light source for exciting the targets, thus generating luminescence signals and a detector for detecting these signals, resulting in a detected signal which comprises a desired signal originating from the targets and a background signal. It moreover comprises a bleaching device for bleaching of at least part of the sources generating the background signal and a processor configured to trigger the bleaching device to start bleaching, and to trigger the light source for exciting the remaining luminescent targets which are not bleached, and to trigger the detector for detecting the luminescence signal of the remaining luminescent targets, so as to generate a measurement signal representative for the quantification of the luminescent targets.

公开(公告)号：US20180172587A1

公开(公告)日：2018-06-21

申请号：US15736414

申请日：2016-06-22

Applicant: IMEC VZW

Inventor： Peter Peumans ,  Liesbet Lagae ,  Willem Van Roy ,  Tim Stakenborg ,  Pol Van Dorpe

IPC: G01N21/64

CPC classification number: G01N21/6408 ,  G01N21/6456 ,  G01N21/648

Abstract: A sensor device for quantifying luminescent targets. The device comprises a light source for exciting the targets, thus generating luminescence signals, and a detector for detecting these signals of the targets in a cell, resulting in a detected signal comprising a desired signal and a background signal. The detector has a spatial cell resolution and/or a time resolution that is so high that only a limited number of targets will be present in the cell when measuring at low concentration and/or that only a limited number of targets add to the cell in between two measurements. A change in the number of targets in the cell can be observed in the detected signal. The device comprises a processor configured to distinguish the desired and the background signal, and to combine the detected signals of the different cells and/or moments in time, to quantify the targets.

公开(公告)号：US09146235B2

公开(公告)日：2015-09-29

申请号：US14570675

申请日：2014-12-15

Applicant: IMEC VZW ,  Katholieke Universiteit Leuven, KU LEUVEN R&D

Inventor： Pol Van Dorpe ,  Sarp Kerman ,  Peter Peumans ,  Willem Van Roy

IPC: G01N21/64 ,  G01N21/77 ,  G01N33/50 ,  G01N33/543

CPC classification number: G01N33/54373 ,  G01N15/1436 ,  G01N15/1456 ,  G01N21/6428 ,  G01N21/6454 ,  G01N21/648 ,  G01N21/7743 ,  G01N2015/1006 ,  G01N2021/6482 ,  G01N2021/7786

Abstract: An integrated fluorescence detector for detecting fluorescent particles is described. An example integrated fluorescence detector comprises a substrate, the substrate comprising an integrated detection element for detecting fluorescence radiation from fluorescent particles upon excitation of the particles with incident excitation radiation. The integrated fluorescence detector also comprises a sensing layer adapted for accommodating fluorescent particles to be sensed. The integrated fluorescence detector further comprises a photonics crystal layer arranged in between the sensing layer and the substrate, the photonics crystal layer comprising an absorption material designed such that the photonics crystal layer is configured for diffracting incident excitation radiation into a lateral direction in which the photonics crystal layer extends for incident excitation radiation having a wavelength within at least 10 nm of the predetermined excitation wavelength.

Abstract translation: 描述了用于检测荧光颗粒的集成荧光检测器。 示例性的集成荧光检测器包括衬底，该衬底包括用于在用入射激发辐射激发颗粒时检测来自荧光颗粒的荧光辐射的集成检测元件。 集成荧光检测器还包括适于容纳要感测的荧光颗粒的感测层。 集成荧光检测器还包括布置在感测层和衬底之间的光子晶体层，光子晶体层包括设计成使得光子晶体层被配置为将入射激发辐射衍射到横向方向的吸收材料，其中光子 晶体层对于具有在预定激发波长的至少10nm内的波长的入射激发辐射延伸。

公开(公告)号：US20150105297A1

公开(公告)日：2015-04-16

申请号：US14512762

申请日：2014-10-13

Applicant: IMEC VZW ,  Katholieke Universiteit Leuven, KU LEUVEN R&D

Inventor： Tim Stakenborg ,  Robert Neely ,  Pol Van Dorpe ,  Johan Hofkens

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  B01L3/5027 ,  B01L2300/0636 ,  B01L2300/0645 ,  C12Q1/6816 ,  G01N33/48721 ,  C12Q2527/113 ,  C12Q2563/113 ,  C12Q2563/116 ,  C12Q2565/629

Abstract: A micro-fluidic device for mapping a DNA or RNA strand labeled at a plurality of specific sites with labels suitable for generating a detection signal when interacting with a detector element, the device comprising: a micro-fluidic channel; and a plurality of detector elements for detecting the labels by acquiring the detection signals, the detector elements being positioned longitudinally along the micro-fluidic channel, each detector element having a width, successive detector elements being separated by an inter-detector gap having a width, wherein the widths of at least two of the detector elements are different and/or wherein the widths at least two of the inter-detector gaps are different.

Abstract translation: 一种微流体装置，用于将在多个特定位点处标记的DNA或RNA链与用于在与检测器元件相互作用时产生检测信号的标签进行映射，所述装置包括：微流体通道; 以及多个检测器元件，用于通过获取检测信号来检测标签，检测器元件沿着微流体通道纵向定位，每个检测器元件具有宽度，连续的检测器元件由具有宽度的检测器间间隔 ，其中所述检测器元件中的至少两个的宽度是不同的，和/或其中所述检测器间间隔中的至少两个的宽度是不同的。

公开(公告)号：US12066372B2

公开(公告)日：2024-08-20

申请号：US17544300

申请日：2021-12-07

Applicant: IMEC VZW

Inventor： Niels Verellen ,  Pol Van Dorpe

IPC: G01N15/1434 ,  G01N15/10

CPC classification number: G01N15/1434 ,  G01N2015/1006

Abstract: According to an aspect of the present inventive concept there is provided a light excitation and collection device for a micro-fluidic system, comprising:



a light source configured to generate excitation light;
a plurality of excitation waveguides, each associated with a flow channel of the micro-fluidic system;
wherein each excitation waveguide is configured to receive and redirect the excitation light towards the flow channel, such that the excitation light is elastically scattered by a sample in the flow channel forming forward and side scattered light; and
wherein the light excitation and collection device further comprises:
at least one forward scattered light collection point; and
at least one side scattered light collection point; and
wherein the forward scattered light collected for all excitation waveguides is detected by a first plurality of light sensitive areas and the side scattered light collected for all excitation waveguides is detected by a second plurality of light sensitive areas, the first and the second pluralities of light sensitive areas form different groups of light sensitive areas.

公开(公告)号：US12000785B2

公开(公告)日：2024-06-04

申请号：US17428607

申请日：2020-02-14

Applicant: IMEC VZW

Inventor： Finub James Shirley ,  Pol Van Dorpe

IPC: G01N21/64 ,  G01N21/49

CPC classification number: G01N21/6454 ,  G01N21/49 ,  G01N2021/6471

Abstract: The present invention provides an apparatus for detecting photoluminescent light emitted from a sample, said apparatus (200; 300) comprising at least one light source (210; 310, 318), which is configured to emit light of a first and a second wavelength towards a sample comprising photoluminescent particles, wherein said first wavelength is an excitation wavelength for inducing photoluminescent light from said photoluminescent particles, and wherein said second wavelength is longer than said first wavelength and for gathering background noise information from said sample. The apparatus further comprises a photo-detector (206) for detecting light incident on the photo-detector (206); and an interference filter (204; 304) arranged on the photo-detector (206), wherein the interference filter (204; 304) is configured to selectively collect and transmit light towards the photo-detector (206) based on an angle of incidence of the light towards the interference filter (204; 304), wherein the interference filter (204; 304) is configured to selectively transmit supercritical angle light from the sample towards the photo-detector (206) and suppress undercritical angle light from the sample.

公开(公告)号：US11754825B2

公开(公告)日：2023-09-12

申请号：US17107974

申请日：2020-12-01

Applicant: IMEC VZW

Inventor： Pol Van Dorpe

IPC: G02B21/06 ,  G02B5/18 ,  G02B21/36 ,  G02B21/02

CPC classification number: G02B21/361 ,  G02B5/1861 ,  G02B21/02 ,  G02B21/367

Abstract: An illuminator, comprising: an illumination waveguide, and a controller; the illumination waveguide being a planar waveguide configured to receive a light wave at a receiving end and guide it to a mirror end; the mirror end comprising a patterned mirror configured to reflect at least part of the light wave back into the illumination waveguide, the patterned mirror comprising a pattern configured to confer a diffraction pattern to the reflected light, the diffraction pattern contributing to an interference pattern, the interference pattern having an evanescent field outside the illumination waveguide, wherein the evanescent field of the interference pattern is configured to illuminate an object in close relation to the illumination waveguide; wherein the controller is configured to control a wavefront of the received light wave and to set a relation between the controlled wavefront and the pattern of the patterned mirror such that the interference pattern forms at least one element of constructive interference for selectively illuminating a portion of the object; wherein the controller is further configured to sequentially change the interference pattern.