公开(公告)号：US20140107331A1

公开(公告)日：2014-04-17

申请号：US14141052

申请日：2013-12-26

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Kenzo Fujimoto ,  Masayuki Ogino ,  Yoshinaga Yoshimura

IPC: C07H21/04 ,  C07H19/073

CPC classification number: C07H21/00 ,  C07H19/073 ,  C07H21/02 ,  C07H21/04

Abstract: The present invention provides a manufacturing method that can easily manufacture a compound known as photoresponsive (photocoupling) nucleic acids at high yield in a shorter period of time than that of the conventional technology. The present invention relates to a method of manufacturing a photoresponsive nucleic acid which includes a step of reacting a nucleic acid having groups represented by the Formula I, the Formula III, the Formula IV, or the Formula V and a compound represented by the Formula II, or reacting a nucleic acid having groups represented by the Formula VI, the Formula VIII, the Formula IX, or the Formula X and a compound represented by the Formula VII by heating them by microwaves in the presence of a metal catalyst, a basic substance, and a solvent.

Abstract translation: 本发明提供了一种制造方法，其可以在比常规技术更短的时间内以高产率容易地制造称为光响应（光耦合）核酸的化合物。 本发明涉及一种制备光响应核酸的方法，其包括使具有由式I，式III，式IV或式V表示的基团的核酸与式II表示的化合物反应的步骤 或通过在金属催化剂，碱性物质的存在下通过微波加热而使具有由式VI，式VIII，式IX或式X表示的基团的核酸和式VII表示的化合物反应 ，和溶剂。

公开(公告)号：US20160326207A1

公开(公告)日：2016-11-10

申请号：US15033422

申请日：2014-10-30

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Kenzo Fujimoto ,  Shigetaka Nakamura

IPC: C07H19/06

CPC classification number: C07H19/06 ,  C07H19/04 ,  C07H19/073 ,  C07H21/04

Abstract: Provided is a means for preventing the inactivation of a photoresponsive nucleic acid probe by suppressing the formation of a photocrosslink between a photoresponsive base having a photocrosslinkable vinyl structure and a photocrosslinkable thymine (T) or uracil (U) base, by substituting with an R group (R being —CN or —CO—R1, where R1 is a saturated or unsaturated straight-chain or branched cyclic or non-cyclic C1-12 hydrocarbon group) the 5 position of a pyrimidine ring of the thymine (T) or uracil (U) base which is photocrosslinkable with the photoresponsive base having a photocrosslinkable vinyl structure.

Abstract translation: 本发明提供通过抑制光可交联乙烯基结构的光响应性碱与光可交联胸腺嘧啶（T）或尿嘧啶（U）碱之间的光交联的形成，通过用R基取代来防止光响应性核酸探针失活的手段 （R为-CN或-CO-R 1，其中R 1为饱和或不饱和的直链或支链环状或非环状C 1-12烃基）胸腺嘧啶（T）或尿嘧啶 U）碱，其与具有可光致交联的乙烯基结构的光响应性碱可光交联。

公开(公告)号：US09359393B2

公开(公告)日：2016-06-07

申请号：US14141052

申请日：2013-12-26

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Kenzo Fujimoto ,  Masayuki Ogino ,  Yoshinaga Yoshimura

IPC: C07H21/02 ,  C07H21/04 ,  C07H21/00 ,  C07H19/073

CPC classification number: C07H21/00 ,  C07H19/073 ,  C07H21/02 ,  C07H21/04

Abstract: The present invention provides a manufacturing method that can easily manufacture a compound known as photoresponsive (photocoupling) nucleic acids at high yield in a shorter period of time than that of the conventional technology. The present invention relates to a method of manufacturing a photoresponsive nucleic acid which includes a step of reacting a nucleic acid having groups represented by the Formula I, the Formula III, the Formula IV, or the Formula V and a compound represented by the Formula II, or reacting a nucleic acid having groups represented by the Formula VI, the Formula VIII, the Formula IX, or the Formula X and a compound represented by the Formula VII by heating them by microwaves in the presence of a metal catalyst, a basic substance, and a solvent.

Abstract translation: 本发明提供了一种制造方法，其可以在比常规技术更短的时间内以高产率容易地制造称为光响应（光耦合）核酸的化合物。 本发明涉及一种制备光响应核酸的方法，其包括使具有由式I，式III，式IV或式V表示的基团的核酸与式II表示的化合物反应的步骤 或通过在金属催化剂，碱性物质的存在下通过微波加热而使具有由式VI，式VIII，式IX或式X表示的基团的核酸和式VII表示的化合物反应 ，和溶剂。