公开(公告)号：US20250163098A1

公开(公告)日：2025-05-22

申请号：US18696694

申请日：2022-09-27

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Masahito Inagaki ,  Fumitaka Hashiya ,  Haruka Hiraoka ,  Naoko Abe

IPC: C07H21/04

Abstract: There is provided a nucleic acid strand cleaving method including a nucleic acid preparation step of preparing a nucleic acid to be cleaved having a structure represented by the following Formula (1), and a cleaving step of reacting the nucleic acid to be cleaved with a cleaving agent to cleave the nucleic acid to be cleaved at the part X of the Formula (1) to generate a nucleic acid having a structure represented by the following Formula (2). The cleaving agent is a metal nanoparticle containing an atom selected from the group consisting of silver, mercury, and cadmium. [Chemical Formula 1] Here, B represents a base, and X represents sulfur or selenium. NucA is composed of at least one nucleotide and is a part of the nucleic acid to be cleaved, and represents a part on the 5′ end side with reference to the X. NucB is composed of at least one nucleotide and is a part of the nucleic acid to be cleaved, and represents a part on the 3′ end side with reference to the X.

公开(公告)号：US20240327444A1

公开(公告)日：2024-10-03

申请号：US18576587

申请日：2022-07-05

Applicant: Japan Science and Technology Agency ,  National University Corporatjion Tokai National Higher Education and Research System

Inventor： Hiroshi Abe ,  Naoko Abe ,  Masahito Inagaki ,  Yasuaki Kimura ,  Fumitaka Hashiya ,  Zhenmin Li ,  Yuko Nakashima

IPC: C07H1/06 ,  A61K31/7105 ,  C07F9/24 ,  C07H17/02 ,  C07H21/00

CPC classification number: C07H1/06 ,  A61K31/7105 ,  C07F9/2458 ,  C07H17/02 ,  C07H21/00

Abstract: A method for purifying a nucleotide-based substance; including a protecting group introduction step of introducing a hydrophobic protecting group represented by the following formula (P1) or (P2) into a nucleotide-based substance to produce a hydrophobic nucleotide-based substance; an isolation and purification step of isolating and purifying the hydrophobic nucleotide-based substance under a hydrophobic environment; and a deprotection step of deprotecting the hydrophobic protecting group from the hydrophobic nucleotide-based substance to produce the nucleotide-based substance,






wherein R1 represents a linear or branched alkyl group having 1 to 30 carbon atoms, R4 represents hydrogen or a linear or branched alkyl group having 1 to 10 carbon atoms, R2, R3, R5 and R6 represent hydrogen, a linear or branched alkyl group having 1 to 10 carbon atoms, or the like, and may be the same or different; and * means a bond with a nucleotide-based substance.