公开(公告)号：US10858392B2

公开(公告)日：2020-12-08

申请号：US15660373

申请日：2017-07-26

Applicant: Kaneka Corporation

Inventor： Shinichi Yoshida ,  Dai Murata

IPC: C07K1/22 ,  C07K14/315 ,  C07K16/00 ,  C07K17/00 ,  C07K16/10

Abstract: An affinity separation matrix includes a water-insoluble base material; and a ligand that is immobilized on the water-insoluble base material, wherein the ligand is an antibody κ chain variable region-binding peptide comprising B5 domain of Protein L derived from Peptostreptococcus magnus 312 strain or a part thereof.

公开(公告)号：US20170327535A1

公开(公告)日：2017-11-16

申请号：US15660373

申请日：2017-07-26

Applicant: Kaneka Corporation

Inventor： Shinichi Yoshida ,  Dai Murata

IPC: C07K1/22 ,  C07K16/00 ,  C07K17/00

CPC classification number: C07K1/22 ,  C07K14/315 ,  C07K16/00 ,  C07K16/1027 ,  C07K17/00 ,  C07K2317/55

Abstract: An affinity separation matrix includes a water-insoluble base material; and a ligand that is immobilized on the water-insoluble base material, wherein the ligand is an antibody κ chain variable region-binding peptide comprising B5 domain of Protein L derived from Peptostreptococcus magnus 312 strain or a part thereof.

公开(公告)号：US20190211082A1

公开(公告)日：2019-07-11

申请号：US16183308

申请日：2018-11-07

Applicant: KANEKA CORPORATION

Inventor： Dai Murata ,  Shinichi Yoshida ,  Kazunobu Minakuchi

IPC: C07K16/06 ,  C07K1/22 ,  B01D15/38 ,  C07K1/36 ,  C07K16/12

CPC classification number: C07K16/065 ,  B01D15/3804 ,  C07K1/22 ,  C07K1/36 ,  C07K16/00 ,  C07K16/1275 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/92

Abstract: A method for purifying an antibody or an antibody fragment containing κ-chain variable region includes adsorbing at least one of the antibody or the antibody fragment onto an affinity separation matrix by contacting a liquid sample with the affinity separation matrix, washing the affinity separation matrix to remove impurities, and separating the at least one of the antibody or the antibody fragment from the affinity separation matrix by using an acetate buffer. The liquid sample includes the at least one of the antibody or the antibody fragment. The affinity separation matrix includes a water-insoluble carrier and a ligand selected from the group consisting of Protein L, a variant of Protein L, a domain of Protein L, and a variant of the domain. The ligand is immobilized on the water-insoluble carrier.

公开(公告)号：US10556944B2

公开(公告)日：2020-02-11

申请号：US14914439

申请日：2014-08-28

Applicant: KANEKA CORPORATION

Inventor： Shinichi Yoshida ,  Dai Murata

IPC: C07K16/12 ,  C07K14/315 ,  C07K1/22

Abstract: An object of the present invention is to provide a Fab region-binding peptide having an excellent ability for binding to a Fab region of IgG, an affinity separation matrix having the peptide as a ligand, and a method for producing a Fab region-containing protein, the method using the affinity separation matrix. Further, another object of the present invention is to provide a DNA encoding for the peptide, a vector containing the DNA, and a transformant which has been transformed by the vector. The Fab region-binding peptide according to the present invention is characterized in having a mutation at a specific site in comparison with wild-type SpG-β1.

公开(公告)号：US20190263870A1

公开(公告)日：2019-08-29

申请号：US16298639

申请日：2019-03-11

Applicant: KANEKA CORPORATION

Inventor： Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira ,  Masayuki Takano ,  Keita Iguchi ,  Yoshiyuki Nakano

IPC: C07K14/195 ,  C07K16/32 ,  C07K14/31 ,  C07K16/06 ,  C07K1/22

Abstract: An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the acidic condition compared with known engineered Protein A ligands. The present invention provides a protein having an affinity for an immunoglobulin, including an amino acid sequence obtained by introducing, into an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, at least one amino acid substitution at any one or more of amino acid residues corresponding to positions 31 to 37 of the A, B and C domains (positions 29 to 35 of the E domain, positions 34 to 40 of the D domain), which are conserved in all the domains, the protein having a lower affinity for an Fab region of an immunoglobulin than a protein having the amino acid sequence before introduction of the substitution.

公开(公告)号：US20190153072A1

公开(公告)日：2019-05-23

申请号：US16259174

申请日：2019-01-28

Applicant: KANEKA CORPORATION

Inventor： Dai Murata ,  Shinichi Yoshida

IPC: C07K16/06 ,  B01D15/38

Abstract: A method for producing an antibody fragment includes preparing a liquid sample that includes the antibody fragment and does not include an Fc fragment, adsorbing the antibody fragment on an affinity separation matrix by contacting the liquid sample with the affinity separation matrix, removing impurities from the affinity separation matrix by washing the affinity separation matrix, and separating the antibody fragment from the affinity separation matrix. The antibody fragment includes a CH1 region and does not include an Fc region. The affinity separation matrix includes a water-insoluble carrier and a ligand immobilized on the water-insoluble carrier. The ligand is Protein G, a Protein G domain, a Protein G variant or a Protein G domain variant.

公开(公告)号：US20190119362A1

公开(公告)日：2019-04-25

申请号：US16176090

申请日：2018-10-31

Applicant: KANEKA CORPORATION

Inventor： Shinichi Yoshida ,  Dai Murata

IPC: C07K16/06 ,  C07K1/22

Abstract: A method for producing a protein includes adsorbing a protein including a κ chain variable region on an insoluble carrier of an affinity separation matrix by contacting a liquid sample including the protein with the affinity separation matrix; washing the affinity separation matrix to remove impurities; separating the protein from the affinity separation matrix by using an acidic buffer; and regenerating the affinity separation matrix by using an alkaline aqueous solution after the protein is separated from the affinity separation matrix. The insoluble carrier includes a ligand immobilized on the insoluble carrier, and the ligand is a κ chain variable region-binding peptide including B5 domain of Protein L derived from Peptostreptococcus magnus 312 strain or a variant of the B5 domain. The adsorbing, the washing, and the separating are repeated 3 or more times.

公开(公告)号：US12240919B2

公开(公告)日：2025-03-04

申请号：US17434039

申请日：2020-02-27

Applicant: KANEKA CORPORATION

Inventor： Takuma Suzuki ,  Dai Murata ,  Masakatsu Nishihachijyo ,  Hisako Yaura

IPC: C07K17/14 ,  C07K1/22

Abstract: The objective of the present invention is to provide a method for efficiently supporting a thiol group-including compound on an insoluble base material. The method for supporting a thiol group-including compound on an insoluble base material according to the present invention is characterized in comprising Step A: treating the thiol group-including compound with a thiol group-including organic reducing agent and an inorganic reducing agent, and Step B: contacting a reaction liquid of said Step A with the insoluble base material.

公开(公告)号：US20190134606A1

公开(公告)日：2019-05-09

申请号：US16183878

申请日：2018-11-08

Applicant: KANEKA CORPORATION

Inventor： Dai Murata ,  Shinichi Yoshida

IPC: B01J20/32 ,  C07K1/22 ,  C07K17/02 ,  B01J20/24 ,  B01J20/282

Abstract: A method for producing an affinity separation matrix includes immobilizing a κ chain variable region-binding peptide on a water-insoluble carrier through a terminal cysteine residue of the κ chain variable region-binding peptide. The cysteine residue is located at an N-terminal or a C-terminal of the κ chain variable region-binding peptide. The κ chain variable region-binding peptide is a ligand having an affinity for a κ chain variable region. The affinity separation matrix includes the ligand and the water-insoluble carrier.

公开(公告)号：US09920098B2

公开(公告)日：2018-03-20

申请号：US14429819

申请日：2013-09-24

Applicant: Kaneka Corporation

Inventor： Shinichi Yoshida ,  Dai Murata ,  Fuminori Konoike ,  Keita Iguchi ,  Tomoyuki Nakaishi ,  Masahiro Hayashi

IPC: B01D15/38 ,  B01J20/26 ,  C07K1/22 ,  C07K14/31 ,  C07K16/06 ,  C07K17/00 ,  C07K16/00 ,  C07K16/12 ,  C07K17/12

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J2220/4856 ,  C07K1/22 ,  C07K16/00 ,  C07K16/065 ,  C07K16/1271 ,  C07K17/00 ,  C07K17/12 ,  C07K2317/21 ,  C07K2317/52 ,  C07K2317/92

Abstract: An object of the present invention is to develop techniques to create novel engineered protein ligands that maximize the binding capacity and binding efficiency to a target molecule of affinity separation matrices on which the protein ligands are immobilized. The present invention provides protein ligands (variants) that can be immobilized on carriers in a manner shown in schematic FIG. 1(4)-(15), as well as antibody affinity separation matrices obtained by immobilizing such a protein ligand on a water-insoluble carrier. The affinity separation matrices are characterized by their excellent binding capacity and binding efficiency to a target molecule.