公开(公告)号：US20190263870A1

公开(公告)日：2019-08-29

申请号：US16298639

申请日：2019-03-11

Applicant: KANEKA CORPORATION

Inventor： Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira ,  Masayuki Takano ,  Keita Iguchi ,  Yoshiyuki Nakano

IPC: C07K14/195 ,  C07K16/32 ,  C07K14/31 ,  C07K16/06 ,  C07K1/22

Abstract: An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the acidic condition compared with known engineered Protein A ligands. The present invention provides a protein having an affinity for an immunoglobulin, including an amino acid sequence obtained by introducing, into an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, at least one amino acid substitution at any one or more of amino acid residues corresponding to positions 31 to 37 of the A, B and C domains (positions 29 to 35 of the E domain, positions 34 to 40 of the D domain), which are conserved in all the domains, the protein having a lower affinity for an Fab region of an immunoglobulin than a protein having the amino acid sequence before introduction of the substitution.

公开(公告)号：US09920098B2

公开(公告)日：2018-03-20

申请号：US14429819

申请日：2013-09-24

Applicant: Kaneka Corporation

Inventor： Shinichi Yoshida ,  Dai Murata ,  Fuminori Konoike ,  Keita Iguchi ,  Tomoyuki Nakaishi ,  Masahiro Hayashi

IPC: B01D15/38 ,  B01J20/26 ,  C07K1/22 ,  C07K14/31 ,  C07K16/06 ,  C07K17/00 ,  C07K16/00 ,  C07K16/12 ,  C07K17/12

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J2220/4856 ,  C07K1/22 ,  C07K16/00 ,  C07K16/065 ,  C07K16/1271 ,  C07K17/00 ,  C07K17/12 ,  C07K2317/21 ,  C07K2317/52 ,  C07K2317/92

Abstract: An object of the present invention is to develop techniques to create novel engineered protein ligands that maximize the binding capacity and binding efficiency to a target molecule of affinity separation matrices on which the protein ligands are immobilized. The present invention provides protein ligands (variants) that can be immobilized on carriers in a manner shown in schematic FIG. 1(4)-(15), as well as antibody affinity separation matrices obtained by immobilizing such a protein ligand on a water-insoluble carrier. The affinity separation matrices are characterized by their excellent binding capacity and binding efficiency to a target molecule.

公开(公告)号：US20160168209A1

公开(公告)日：2016-06-16

申请号：US14429819

申请日：2013-09-24

Applicant: KANEKA CORPORATION

Inventor： Shinichi Yoshida ,  Dai Murata ,  Fuminori Konoike ,  Keita Iguchi ,  Tomoyuki Nakaishi ,  Masahiro Hayashi

IPC: C07K14/31 ,  C07K16/12 ,  C07K17/12 ,  C07K1/22

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J2220/4856 ,  C07K1/22 ,  C07K16/00 ,  C07K16/065 ,  C07K16/1271 ,  C07K17/00 ,  C07K17/12 ,  C07K2317/21 ,  C07K2317/52 ,  C07K2317/92

Abstract: An object of the present invention is to develop techniques to create novel engineered protein ligands that maximize the binding capacity and binding efficiency to a target molecule of affinity separation matrices on which the protein ligands are immobilized. The present invention provides protein ligands (variants) that can be immobilized on carriers in a manner shown in schematic FIG. 1(4)-(15), as well as antibody affinity separation matrices obtained by immobilizing such a protein ligand on a water-insoluble carrier. The affinity separation matrices are characterized by their excellent binding capacity and binding efficiency to a target molecule.

Abstract translation: 本发明的一个目的是开发技术来产生新的工程化蛋白质配体，其最大限度地增加了与蛋白质配体固定在其上的亲和分离基质的靶分子的结合能力和结合效率。 本发明提供蛋白质配体（变体），其可以以示意图所示的方式固定在载体上。 1（4） - （15），以及通过将这种蛋白质配体固定在水不溶性载体上获得的抗体亲和力分离基质。 亲和力分离基质的特征在于其与靶分子的优异的结合能力和结合效率。