公开(公告)号：US20220283116A1

公开(公告)日：2022-09-08

申请号：US17632613

申请日：2020-08-03

Applicant: King Abdullah University of Science and Technology

Inventor： Shofarul Wustoni ,  Sahika Inal

IPC: G01N27/414 ,  G01N27/327 ,  G01N27/333 ,  G01N27/31 ,  G01N27/447

Abstract: Devices for detecting an analyte in a sample suspected of containing the analyte, are provided. The devices include bio-functional, nanostructured, isoporous membranes (BNIM) integrated organic electrochemical transistor (OECT), herein BNIM-OECT, for the rapid and sensitive detection of the presence of an analyte of interest, in a sample, for example, a biological sample. The membrane (i.e., BNIM) is physically separated from the OECT channel therefore the electronic device can be used multiple times. The isoporous membrane is functionalized to include a binding partner for the analyte being detected. The BNIM-OECT can be used for disease detection, by functionalizing the BNIM-OECT with a binding partner to an analyte associated with the disease, applying a collected biological sample to the BNIM-OECT. A decrease in channel current as a result of analyte binding to its binding partner on the isoporous membrane indicates the presence of the analyte in the sample.

公开(公告)号：US20250027901A1

公开(公告)日：2025-01-23

申请号：US18711844

申请日：2022-11-18

Applicant: King Abdullah University of Science and Technology

Inventor： Sahika Inal ,  Stefan T. Arold ,  Raik Grunberg ,  Shofarul Wustoni ,  Keying Guo ,  Anil Koklu ,  Miriam Escarlet Diaz Galicia

IPC: G01N27/414 ,  G01N27/327 ,  G01N33/543 ,  G01N33/569

Abstract: Devices and methods of analyte detection using AC electrokinetic/electrohydrodynamic forces combined with an OECT-based immunosensor are disclosed. An analyte binding agent, for example, a nanobody-functionalized organic electrochemical transistor (OECT) is incorporated with the micro-stirring effect of alternating current electrothermal flow (ACET) for the ultrarapid detection of single-molecule-to-nanomolar levels of the analyte. The ACET flow is induced by a biased AC electrical field can rapidly convect the analyte onto concentric gate electrodes within a minute, and the analyte is captured via recognition units that bind the analyte binding agent while sweeping nonspecific ally bound analyte away from the surface.

公开(公告)号：US12292406B2

公开(公告)日：2025-05-06

申请号：US17632613

申请日：2020-08-03

Applicant: King Abdullah University of Science and Technology

Inventor： Shofarul Wustoni ,  Sahika Inal

IPC: G01N27/414 ,  G01N27/31 ,  G01N27/327 ,  G01N27/333 ,  G01N27/447

Abstract: Devices for detecting an analyte in a sample suspected of containing the analyte, are provided. The devices include bio-functional, nanostructured, isoporous membranes (BNIM) integrated organic electrochemical transistor (OECT), herein BNIM-OECT, for the rapid and sensitive detection of the presence of an analyte of interest, in a sample, for example, a biological sample. The membrane (i.e., BNIM) is physically separated from the OECT channel therefore the electronic device can be used multiple times. The isoporous membrane is functionalized to include a binding partner for the analyte being detected. The BNIM-OECT can be used for disease detection, by functionalizing the BNIM-OECT with a binding partner to an analyte associated with the disease, applying a collected biological sample to the BNIM-OECT. A decrease in channel current as a result of analyte binding to its binding partner on the isoporous membrane indicates the presence of the analyte in the sample.

公开(公告)号：US20240280570A1

公开(公告)日：2024-08-22

申请号：US18004117

申请日：2021-07-02

Applicant: King Abdullah University of Science and Technology

Inventor： Sahika Inal ,  Stefan T. Arold ,  Raik Grunberg ,  Shofarul Wustoni ,  Keying Guo ,  Anil Koklu

IPC: G01N33/543

CPC classification number: G01N33/5438 ,  G01N2333/165 ,  G01N2333/47

Abstract: Organic electrochemical transistor (OECT)-based immunosensors, methods of making and methods of use thereof are provided herein. The immunosensor includes an OECT and a biorecognition layer. The biorecognition layer is preferably integrated on the gate electrode of the OECT. The biorecognition layer includes a self-assembled monolayer (SAM) of organic molecules, a linker, and a biorecognition element preferably, a nanobody. In a preferred embodiment, the organic molecules forming the SAM include thiols. The disclosed methods can be used to make OECT devices containing a nanobody whose binding partner is any analyte of interest, such as SARS-2 RBD, S1 or the virus itself.