公开(公告)号：US20230210960A1

公开(公告)日：2023-07-06

申请号：US18000996

申请日：2021-06-03

Applicant: Korea University Research and Business Foundation

Inventor： Young Sik LEE ,  Seung Jae LEE ,  Jae Sang HONG ,  Do Hwan LIM

IPC: A61K38/45 ,  C12N15/113 ,  C12N9/10 ,  C12N15/63

CPC classification number: A61K38/45 ,  C12N15/113 ,  C12N9/1007 ,  C12Y201/01057 ,  C12N15/63

Abstract: The present disclosure relates to a novel use of CMTR1 having the activity of enhancing the production and function of siRNA, and more particularly, to a composition for enhancing the production of siRNA including a cap1 2′-O-ribose methyltransferase (CMTR1) protein as an active ingredient, and a composition for enhancing the gene silencing activity by siRNA, and also to a method for producing siRNA for gene silencing in vitro. According to the present disclosure, the CMTR1 may enhance the production of siRNA for gene silencing and at the same time, ultimately enhance the production and function of siRNA without artificial chemical modification of siRNA by enhancing the formation of holo-RNA-induced silencing complex (RISC) that acts on silencing of a target gene in an RNAi mechanism. Therefore, the CMTR1 of the present disclosure can be usefully used in the development of pharmaceuticals using siRNA as a therapeutic agent.