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    KITS AND METHODS FOR GENERATING 5' CAPPED RNA
    3.
    发明申请
    KITS AND METHODS FOR GENERATING 5' CAPPED RNA 有权

    公开(公告)号：US20140363876A1

    公开(公告)日：2014-12-11

    申请号：US14471649

    申请日：2014-08-28

    Applicant: CELLSCRIPT, LLC

    Inventor： Jerome Jendrisak ,  Ronald Meis ,  Gary Dahl

    IPC: C12P19/34

    CPC classification number: C12P19/34 ,  C12N9/1007 ,  C12N9/1241 ,  C12N15/1072 ,  C12N15/1075 ,  C12N15/1096 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/317 ,  C12N2320/51 ,  C12Y201/01056 ,  C12Y201/01057 ,  C12Y207/07019 ,  C12Y207/0705

    Abstract: The present invention relates to kits and methods for efficiently generating 5′ capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production of 5′-capped RNA having a modified cap nucleotide and for in vitro or in vivo production of polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate, and methods and kits for using a capping enzyme system and modified cap nucleotides for labeling uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate with detectable dye or enzyme moieties.

    Kits and methods for generating 5' capped RNA
    4.
    发明申请
    Kits and methods for generating 5' capped RNA 审中-公开
    用于产生5'封端RNA的试剂盒和方法

    公开(公告)号：US20070281336A1

    公开(公告)日：2007-12-06

    申请号：US11787352

    申请日：2007-04-16

    Applicant: Jerome Jendrisak ,  Ronald Meis ,  Gary Dahl

    Inventor： Jerome Jendrisak ,  Ronald Meis ,  Gary Dahl

    IPC: C12P19/34 ,  C07H21/00 ,  C07H21/02 ,  C12N5/04 ,  C12P21/00 ,  C12N5/10 ,  C12N9/00 ,  C12N9/10

    CPC classification number: C12P19/34 ,  C12N9/1007 ,  C12N9/1241 ,  C12N15/1072 ,  C12N15/1075 ,  C12N15/1096 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/317 ,  C12N2320/51 ,  C12Y201/01056 ,  C12Y201/01057 ,  C12Y207/07019 ,  C12Y207/0705

    Abstract: The present invention relates to kits and methods for efficiently generating 5′ capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. The invention is used to obtain novel compositions of such modified-nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production of 5′-capped RNA having a modified cap nucleotide and for in vitro or in vivo production of polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA for a variety of research, therapeutic, and commercial applications. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate, such as RNA synthesized in vitro or obtained from a biological source, including prokaryotic mRNA that is in a mixture with other prokaryotic and/or eukaryotic nucleic acids. The method for capturing modified-nucleotide-capped RNA also provides methods and kits for obtaining only type-specific or condition-specific modified-nucleotide-capped RNA by cap-dependent subtraction of that portion of the captured modified-nucleotide-capped RNA in cells of one type or condition that is the same as RNA in cells of another type or condition. The invention further provides methods and kits for using a capping enzyme system and modified cap nucleotides for labeling uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate with detectable dye or enzyme moieties.

    Abstract translation: 本发明涉及用于有效产生具有修饰的帽核苷酸的5'封端RNA并用于这种经修饰的核苷酸封端的RNA分子的试剂盒和方法。 本发明用于获得这种经修饰的核苷酸封端RNA分子的新组合物。 特别地，本发明提供了使用修饰的帽核苷酸和封端酶系统（例如痘病毒覆盖酶）对RNA进行封端的试剂盒和方法。 本发明用于体外生产具有修饰的帽核苷酸的5'上限的RNA，并且通过体外或体内翻译这些经修饰的核苷酸封端的RNA进行多种研究，体外或体内产生多肽 ，治疗和商业应用。 本发明还提供用于捕获或分离未包封的RNA的方法和试剂盒，其包含初级RNA转录物或具有5'-二磷酸的RNA，例如在体外合成或从生物来源获得的RNA，包括与其他原核生物混合的原核mRNA 和/或真核的核酸。 用于捕获修饰的核苷酸加帽的RNA的方法还提供用于仅通过Cap依赖性减去细胞中捕获的修饰核苷酸加帽的RNA的那部分获得仅特定类型或条件特异性修饰的核苷酸加帽的RNA的方法和试剂盒 与另一类型或病症细胞中的RNA相同的一种类型或病症。 本发明还提供了使用封端酶系统和修饰的帽核苷酸标记未封端RNA的方法和试剂盒，其包含具有可检测染料或酶部分的具有5'-二磷酸的初级RNA转录物或RNA。

    Kits and methods for generating 5′ capped RNA
    5.
    发明授权
    Kits and methods for generating 5′ capped RNA 有权
    用于产生5'封端RNA的试剂盒和方法

    公开(公告)号：US09115380B2

    公开(公告)日：2015-08-25

    申请号：US14666805

    申请日：2015-03-24

    Applicant: CELLSCRIPT, LLC

    Inventor： Jerome Jendrisak ,  Ronald Meis ,  Gary Dahl

    IPC: C12P19/34 ,  C12P21/06 ,  C12N9/12 ,  C12N9/10

    CPC classification number: C12P19/34 ,  C12N9/1007 ,  C12N9/1241 ,  C12N15/1072 ,  C12N15/1075 ,  C12N15/1096 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/317 ,  C12N2320/51 ,  C12Y201/01056 ,  C12Y201/01057 ,  C12Y207/07019 ,  C12Y207/0705

    Abstract: The present invention relates to kits and methods for efficiently generating 5′ capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production of 5′-capped RNA having a modified cap nucleotide and for in vitro or in vivo production of polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate, and methods and kits for using a capping enzyme system and modified cap nucleotides for labeling uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate with detectable dye or enzyme moieties.

    Abstract translation: 本发明涉及用于有效产生具有修饰的帽核苷酸的5'封端RNA并用于这种经修饰的核苷酸封端的RNA分子的试剂盒和方法。 特别地，本发明提供了使用修饰的帽核苷酸和封端酶系统（例如痘病毒覆盖酶）对RNA进行封端的试剂盒和方法。 本发明用于通过体外或体内翻译这些经修饰的核苷酸加帽的RNA来体外生产具有修饰的帽核苷酸的5'-加帽的RNA，以及体外或体内产生多肽。 本发明还提供用于捕获或分离包含初级RNA转录物或具有5'-二磷酸的RNA的未封端RNA的方法和试剂盒，以及使用封端酶系统和修饰的帽核苷酸用于标记包含初级RNA转录物或RNA的未加帽RNA的方法和试剂盒 具有可检测的染料或酶部分的5'-二磷酸酯。

    KITS AND METHODS FOR GENERATING 5' CAPPED RNA
    6.
    发明申请
    KITS AND METHODS FOR GENERATING 5' CAPPED RNA 审中-公开
    用于产生5'CAPPED RNA的基因座和方法

    公开(公告)号：US20150191760A1

    公开(公告)日：2015-07-09

    申请号：US14666805

    申请日：2015-03-24

    Applicant: CELLSCRIPT, LLC

    Inventor： Jerome Jendrisak ,  Ronald Meis ,  Gary Dahl

    IPC: C12P19/34 ,  C12N9/10 ,  C12N9/12

    CPC classification number: C12P19/34 ,  C12N9/1007 ,  C12N9/1241 ,  C12N15/1072 ,  C12N15/1075 ,  C12N15/1096 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/317 ,  C12N2320/51 ,  C12Y201/01056 ,  C12Y201/01057 ,  C12Y207/07019 ,  C12Y207/0705

    Abstract: The present invention relates to kits and methods for efficiently generating 5′ capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production of 5′-capped RNA having a modified cap nucleotide and for in vitro or in vivo production of polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate, and methods and kits for using a capping enzyme system and modified cap nucleotides for labeling uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate with detectable dye or enzyme moieties.

    Abstract translation: 本发明涉及用于有效产生具有修饰的帽核苷酸的5'封端RNA并用于这种经修饰的核苷酸封端的RNA分子的试剂盒和方法。 特别地，本发明提供了使用修饰的帽核苷酸和封端酶系统（例如痘病毒覆盖酶）对RNA进行封端的试剂盒和方法。 本发明用于通过体外或体内翻译这些经修饰的核苷酸加帽的RNA来体外生产具有修饰的帽核苷酸的5'-加帽的RNA，以及体外或体内产生多肽。 本发明还提供用于捕获或分离包含初级RNA转录物或具有5'-二磷酸的RNA的未封端RNA的方法和试剂盒，以及使用封端酶系统和修饰的帽核苷酸用于标记包含初级RNA转录物或RNA的未加帽RNA的方法和试剂盒 具有可检测的染料或酶部分的5'-二磷酸酯。

    Kits and methods for generating 5′ capped RNA
    7.
    发明授权
    Kits and methods for generating 5′ capped RNA 有权
    用于产生5'封端RNA的试剂盒和方法

    公开(公告)号：US08846348B2

    公开(公告)日：2014-09-30

    申请号：US14185384

    申请日：2014-02-20

    Applicant: CellScript, LLC

    Inventor： Jerome Jendrisak ,  Ronald Meis ,  Gary Dahl

    IPC: C12P19/34 ,  C12P21/06 ,  C12N15/10

    CPC classification number: C12P19/34 ,  C12N9/1007 ,  C12N9/1241 ,  C12N15/1072 ,  C12N15/1075 ,  C12N15/1096 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/317 ,  C12N2320/51 ,  C12Y201/01056 ,  C12Y201/01057 ,  C12Y207/07019 ,  C12Y207/0705

    Abstract: The present invention relates to kits and methods for efficiently generating 5′ capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production of 5′-capped RNA having a modified cap nucleotide and for in vitro or in vivo production of polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate, and methods and kits for using a capping enzyme system and modified cap nucleotides for labeling uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate with detectable dye or enzyme moieties.

    Abstract translation: 本发明涉及用于有效产生具有修饰的帽核苷酸的5'封端RNA并用于这种经修饰的核苷酸封端的RNA分子的试剂盒和方法。 特别地，本发明提供了使用修饰的帽核苷酸和封端酶系统（例如痘病毒覆盖酶）对RNA进行封端的试剂盒和方法。 本发明用于通过体外或体内翻译这些经修饰的核苷酸加帽的RNA来体外生产具有修饰的帽核苷酸的5'-加帽的RNA，以及体外或体内产生多肽。 本发明还提供用于捕获或分离包含初级RNA转录物或具有5'-二磷酸的RNA的未封端RNA的方法和试剂盒，以及使用封端酶系统和修饰的帽核苷酸用于标记包含初级RNA转录物或RNA的未加帽RNA的方法和试剂盒 具有可检测的染料或酶部分的5'-二磷酸酯。

    NEW USE OF CMTR1 HAVING SIRNA PRODUCTION AND FUNCTION ENHANCING ACTIVITY
    9.
    发明公开
    NEW USE OF CMTR1 HAVING SIRNA PRODUCTION AND FUNCTION ENHANCING ACTIVITY 审中-公开

    公开(公告)号：US20230210960A1

    公开(公告)日：2023-07-06

    申请号：US18000996

    申请日：2021-06-03

    Applicant: Korea University Research and Business Foundation

    Inventor： Young Sik LEE ,  Seung Jae LEE ,  Jae Sang HONG ,  Do Hwan LIM

    IPC: A61K38/45 ,  C12N15/113 ,  C12N9/10 ,  C12N15/63

    CPC classification number: A61K38/45 ,  C12N15/113 ,  C12N9/1007 ,  C12Y201/01057 ,  C12N15/63

    Abstract: The present disclosure relates to a novel use of CMTR1 having the activity of enhancing the production and function of siRNA, and more particularly, to a composition for enhancing the production of siRNA including a cap1 2′-O-ribose methyltransferase (CMTR1) protein as an active ingredient, and a composition for enhancing the gene silencing activity by siRNA, and also to a method for producing siRNA for gene silencing in vitro. According to the present disclosure, the CMTR1 may enhance the production of siRNA for gene silencing and at the same time, ultimately enhance the production and function of siRNA without artificial chemical modification of siRNA by enhancing the formation of holo-RNA-induced silencing complex (RISC) that acts on silencing of a target gene in an RNAi mechanism. Therefore, the CMTR1 of the present disclosure can be usefully used in the development of pharmaceuticals using siRNA as a therapeutic agent.

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