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公开(公告)号：US12099185B2
公开(公告)日：2024-09-24
申请号：US17285479
申请日：2019-10-11
Applicant: Leica Microsystems CMS GmbH
Inventor： Christian Schumann , Ronja Capellmann , Alexander Weiss
CPC classification number: G02B27/0068 , G02B21/02
Abstract: A method is useable for correcting a spherical aberration of a microscope having an objective and a cover slip or object carrier, the objective having a correction element for correcting the spherical aberration. The method includes: ascertaining, by the microscope, an index of refraction of an optical medium bordering the cover slip or object carrier and/or a thickness of the cover slip or object carrier along an optical axis of the objective, ascertaining the spherical aberration based on the index of refraction of the optical medium and/or the thickness of the cover slip or object carrier, and ascertaining, based on the spherical aberration, a positioning variable, by which the correction element is adjusted so that the spherical aberration is corrected.
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公开(公告)号：US11971531B2
公开(公告)日：2024-04-30
申请号：US17285103
申请日：2019-10-18
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Alexander Weiss , Christian Schumann , Ronja Capellmann
CPC classification number: G02B21/34 , G01B11/06 , G02B21/02 , G02B21/0088
Abstract: A method is useable for determining a thickness of a cover slip or object carrier in a microscope, which has an objective facing toward a sample chamber. Two optical media border two opposing surfaces of the cover slip or object carrier and form two partially reflective interfaces, which are arranged at different distances from the objective. The method includes: deflecting a measurement light beam by the objective with oblique incidence on the cover slip or object carrier; generating two reflection light beams spatially separated from one another by the measurement light beam being partially reflected on each of the two interfaces; receiving the two reflection light beams by the objective and conducting them onto a position-sensitive detector; registering the incidence locations on the position-sensitive detector; and determining the thickness of the cover slip or object carrier based on the registered incidence locations.
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公开(公告)号：US11703672B2
公开(公告)日：2023-07-18
申请号：US17292013
申请日：2019-11-08
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Christian Schumann
CPC classification number: G02B21/14 , G02B21/125 , G02B21/367
Abstract: A microscopic transmitted light contrasting method includes illuminating a sample through asymmetrical first and second illumination pupils and imaging the sample through asymmetrical first and second detection pupil in order to generate, respectively, first and second partial images. The first illumination pupil and the first detection pupil, as well as the second illumination pupil and the second detection pupil, are arranged pivoted in relation to one another and partially overlapping in projection on a plane perpendicular to an optical axis in such a way that first and third regions of an angular space are in a bright field and second and fourth regions of the angular space are in a dark field, and the first and second partial images each have a bright and a dark field component. An image of the sample is generated from the first and second partial images.
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公开(公告)号：US20210373307A1
公开(公告)日：2021-12-02
申请号：US17285104
申请日：2019-10-18
Applicant: Leica Microsystems CMS GmbH
Inventor： Christian Schumann , Tobias Bauer
Abstract: A method is useable for digitally correcting an optical image of a sample by a microscope that has a cover slip covering the sample. The method includes: determining, by the microscope, an index of refraction of an optical medium bordering the cover slip, a tilt of the cover slip, and/or a thickness of the cover slip; ascertaining an imaging error to be corrected in the form of a pupil function based on the index of refraction of the optical medium, the tilt of the cover slip, and/or the thickness of the cover slip; carrying out imaging of the sample by the microscope; and digitally correcting image data captured by the imaging of the sample based on the pupil function.
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5.发明申请公开(公告)号：US20190250390A1
公开(公告)日：2019-08-15
申请号：US16098472
申请日：2017-05-02
Applicant: Leica Microsystems CMS GmbH
Inventor： Florian Fahrbach , Frank Sieckmann , Christian Schumann , Oliver Schlicker
CPC classification number: G02B21/0076 , G02B21/0032 , G02B21/16 , G02B21/367 , G02B27/58 , G02B2207/113 , G02B2207/129
Abstract: A microscope includes illumination optics for fluorescence excitation of point light sources of a sample, detection optics and a camera having a sensor. A density of the point light sources is kept low so as to minimize a crossover of point light sources that are behind or close to one another in each image captured by the camera. A means for subdividing a detection aperture into individual sub-apertures is provided in a beam path of the detection optics such that images generated by the individual sub-apertures on the sensor of the camera depict an object volume from different spatial directions.
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Patent Agency Ranking
公开(公告)号：US20180307021A1
公开(公告)日：2018-10-25
申请号：US15956788
申请日：2018-04-19
Applicant: Leica Microsystems CMS GmbH
Inventor： Christian Schulz , Christian Schumann
Abstract: An immersion objective lens for a microscope includes a first lens group having positive refractive power, a second lens group having positive refractive power, a third lens group having negative refractive power and a fourth lens group having positive refractive power disposed in this sequence from the object side. The second lens group is moveable along an optical axis so as to achieve a corrective effect with respect to a spherical aberration, such that a sum of a distance between the second lens group and the first lens group and of a distance between the second lens group and the third lens group is constant. The corrective effect of the second lens group is predetermined such that the spherical aberration is minimized for a light incidence that corresponds to a mean numerical aperture that lies between zero and a nominal aperture of the immersion objective lens.
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公开(公告)号：US10025081B2
公开(公告)日：2018-07-17
申请号：US15035067
申请日：2014-10-30
Applicant: Leica Microsystems CMS GmbH
Inventor： Peter Euteneuer , Christian Schumann
Abstract: An objective and an illumination unit for selectable generation of an orthoscopic beam path proceeding through the objective for pointlike scanning illumination, and of a conoscopic beam path proceeding through the objective for evanescent illumination of an object are disclosed. The illumination unit has a light source for generating illuminating rays along an illuminating beam path; a displacement unit for deflecting the illuminating beam path; a scanning eyepiece, placed after the displacement unit for focusing the illuminating rays into an image plane of the scanning eyepiece; and a mirror surface arranged in the image plane of the scanning eyepiece, having a transparent region for generating the orthoscopic beam path and having an at least partly reflective region facing toward the scanning eyepiece for generating the conoscopic beam path from the illuminating beam path, the image plane is located in a plane conjugated with the exit pupil.
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公开(公告)号：US12228719B2
公开(公告)日：2025-02-18
申请号：US17636034
申请日：2019-08-27
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Christian Schumann , Christian Schulz , Arnold Mueller-Rentz , Andreas Lotter
Abstract: An optical imaging device for a microscope includes an objective and an optical system configured to interact with the objective for optically imaging an object selectively in a first operating mode and a second operating mode. The optical system includes a first optical subsystem associated with the first operating mode, and a second optical subsystem associated with the second operating mode. The first optical subsystem is configured to form a first image of the object with a first magnification. The second optical subsystem is configured to form a second image of the object with a second magnification that is less than the first magnification. The second optical subsystem includes an optical module insertable into the optical path for selecting the second operating mode. The optical module includes a lens element with a positive refractive power.
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公开(公告)号：US12228716B2
公开(公告)日：2025-02-18
申请号：US17638213
申请日：2019-09-02
Applicant: Leica Microsystems CMS GmbH
Inventor： Christian Schumann
Abstract: A microscope includes: a motorized object stage for moving an object; an optical imaging system for forming an optical image of a plane (OE) in which the object is to be optically imaged; an optical scanning unit for moving the plane (OE) to be optically imaged by the optical imaging system relative to the optical imaging system; an image sensor for detecting the optical image of the plane (OE) formed by the optical imaging system; and a controller for controlling the motorized object stage and the optical scanning unit for simultaneously moving the object and the plane (OE) in a same direction relative to the optical imaging system while the optical image is being detected by the image sensor.
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公开(公告)号：US12038570B2
公开(公告)日：2024-07-16
申请号：US17369972
申请日：2021-07-08
Applicant: Leica Microsystems CMS GmbH
Inventor： Christian Schumann , Christian Schulz , Alexander Weiss
CPC classification number: G02B21/33 , G02B21/0048 , G02B21/006
Abstract: A microscope includes: an optical system including an immersion objective for an immersion medium of a predetermined refractive index; an aperture stop; and a processor for setting an immersion-free imaging mode in which the optical system is operated without immersion medium. The processor controls the aperture stop in the immersion-free imaging mode to set a numerical aperture of the immersion objective to a reduced value which is lower than a nominal value of the numerical aperture, the numerical aperture being equal to the nominal value when the optical system is operated using the immersion medium without reducing the numerical aperture by the aperture stop. The processor controls the optical system in accordance with the reduced value of the numerical aperture in the immersion-free imaging mode to generate at least one image representing the overview image of the sample.
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