公开(公告)号：US20160320301A1

公开(公告)日：2016-11-03

申请号：US15104997

申请日：2014-12-17

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Werner KNEBEL ,  Wernher FOUQUET ,  Frank SIECKMANN

IPC: G01N21/59 ,  G01N21/64 ,  G02B21/00 ,  G01N21/47

Abstract: The invention relates to a method for tomographic investigation of a sample (9), in which method a sample (9) is illuminated with an illuminating light bundle (3) and in which a transmitted light bundle (10) that contains the light of the illuminating light bundle (3) transmitted through the sample (9) is detected with a transmission detector (13). The invention further relates to an apparatus for tomographic investigation of a sample (9). Provision is made that the illuminating light bundle (3) and the transmitted light bundle (10) pass in opposite propagation directions through the same objective (7).

Abstract translation: 本发明涉及一种用于样品（9）的断层摄影研究的方法，其中样品（9）用照明光束（3）照射，并且其中包含所述样品（9）的光的透射光束（10） 用透射检测器（13）检测通过样品（9）透射的照明光束（3）。 本发明还涉及一种用于样品（9）的层析成像研究的装置。 设置照明光束（3）和透射光束（10）通过相同的物镜（7）在相反的传播方向上通过。

公开(公告)号：US20210063714A1

公开(公告)日：2021-03-04

申请号：US16977820

申请日：2019-02-20

Applicant: Leica Microsystems CMS GmbH

Inventor： Florian FAHRBACH ,  Werner KNEBEL ,  Peter POTT ,  Wernher FOUQUET

IPC: G02B21/00 ,  G02B21/33 ,  G02B21/24 ,  G01N21/64 ,  G01N21/47

Abstract: An optical device for illuminating a sample located in a sample volume with illumination light and for detecting scattered and/or fluorescent light from the sample includes an optical illumination assembly, an optical detection assembly and at least one attachment element. The optical illumination assembly is configured to transmit the illumination light along an illumination path into the sample volume. The optical detection assembly is configured to collect and relay the scattered and/or fluorescent light from the sample volume along a detection path. At least portions of the illumination path and/or of the detection path extend in the at least one attachment element.

公开(公告)号：US20160153892A1

公开(公告)日：2016-06-02

申请号：US14899157

申请日：2014-06-18

Applicant: Leica Microsystems CMS GmbH

Inventor： Werner KNEBEL ,  Wernher FOUQUET ,  Frank SIECKMANN

IPC: G01N21/13 ,  G02B21/26 ,  G02B21/00 ,  G02B21/06

CPC classification number: G01N21/13 ,  G01N2021/135 ,  G02B21/0024 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/06 ,  G02B21/26 ,  G02B21/34 ,  G02B21/367

Abstract: The invention relates to a method for microscopic investigation of a plurality of samples. The method contains the step of arranging the samples in a sample holder that is movable, in particular in motorized and/or automatic fashion, relative to a sample illumination position in such a way that at least one of the samples is respectively successively positionable in the sample illumination position, a clearance for a deflection means respectively remaining adjacent to the sample that is currently located in the sample illumination position; the step of focusing a light stripe with an illumination objective; the step of deflecting the light stripe, once it has passed through the illumination objective, with the deflection means in such a way that the light stripe propagates at an angle different from zero degrees with respect to the optical axis of the illumination objective and has a focus in the sample illumination position; and the step of successively positioning the samples, retained with the sample holder, in the sample illumination position, and detecting the detected light emerging from the sample respectively located in the sample illumination position. The invention furthermore relates to an optical apparatus having a sample holder that holds a plurality of samples and is supported movably, in particular in motorized and/or automatic fashion, relative to a sample illumination position in such a way that at least one of the samples is respectively successively positionable in the sample illumination position.

Abstract translation: 本发明涉及一种用于多个样品的微观研究的方法。 该方法包括将样品布置在样品保持器中，该样品保持器相对于样品照射位置，特别是以电动和/或自动的方式是可移动的，使得至少一个样品分别可连续定位在 样本照明位置，分别保持与当前位于样本照明位置的样本相邻的偏转装置的间隙; 将光条与照明物体对焦的步骤; 一旦透过照明物镜，偏转装置以使光条以相对于照明物镜的光轴为零度的角度传播的方式偏转光条的步骤，并且具有 聚焦在样品照明位置; 以及将样本保持器保留的样本连续定位在样本照射位置的步骤，以及检测从分别位于样本照明位置的样品出射的检测到的光。 本发明还涉及一种光学装置，其具有保持多个样品的样品保持器，并且以相对于样品照射位置可移动地尤其以机动和/或自动的方式被支撑，使得至少一个样品 分别连续定位在样本照明位置。

公开(公告)号：US20230175963A1

公开(公告)日：2023-06-08

申请号：US17994434

申请日：2022-11-28

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Benjamin DEISSLER ,  Christian SCHUMANN ,  Kai RITSCHEL ,  Mate BELJAN ,  Wernher FOUQUET ,  Gheorghe COJOC

IPC: G01N21/64

CPC classification number: G01N21/64 ,  G01N2201/127

Abstract: A method of analyzing a mixed fluorescence response of a plurality of fluorophores in a microscopic sample includes reconstructing individual fluorescence responses from a mixed fluorescence response using spectral un-mixing based on reference emission spectra for fluorophores to be reconstructed, and a procedure for determining and validating reference emission spectra including providing a plurality of image acquisition settings for a sequence of images of the sample equal to, or greater than, the plurality of fluorophores and including an illumination setting for each image, acquiring the sequence of images using the plurality of image acquisition settings and storing each image together with the corresponding illumination setting, determining candidate reference emission spectra for the fluorophores to be reconstructed from the sequence of images of the sample using one or more reference emission spectra determination algorithms, and conditionally using the candidate reference emission spectra as the reference emission spectra in the spectral un-mixing.

公开(公告)号：US20160048012A1

公开(公告)日：2016-02-18

申请号：US14778926

申请日：2014-03-20

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Werner KNEBEL ,  Wernher FOUQUET ,  Frank SIECKMANN

IPC: G02B21/32 ,  G02B26/08 ,  G02B21/00

CPC classification number: G02B21/32 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/367 ,  G02B26/0816

Abstract: The invention relates to a method in which a sample is manipulated with manipulation light, and in which the sample is imaged by means of the SPIM technique under illumination with illumination light, in particular excitation light for fluorescence excitation, in the form of an illumination light sheet. The method is notable for the fact that both the manipulation light and the illumination light are focused by the same objective that is arranged in an objective working position, or by different objectives that are brought successively into an objective working position; and that the manipulation light and/or the illumination light, after passing through the objective, is diverted by means of a diverting device in such a way that it propagates at an angle different from zero degrees with respect to the optical axis of the objective.

Abstract translation: 本发明涉及一种方法，其中用操作光对样品进行操作，并且其中采用照明光照射下的SPIM技术成像样品，特别是用于荧光激发的激发光，以照明光的形式 片。 该方法的特征在于，操作光和照明光均被设置在目标工作位置的相同目标或通过连续进入目标工作位置的不同目标聚焦; 并且操纵光和/或照明光在通过物镜之后通过转向装置转向，使得其以相对于物镜的光轴不成零的角度传播。