公开(公告)号：US10132754B2

公开(公告)日：2018-11-20

申请号：US15037451

申请日：2014-11-19

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Vishnu Vardhan Krishnamachari ,  Volker Seyfried ,  William C. Hay ,  Manuel Kremer

IPC: G01N21/00 ,  G01N21/65 ,  G02B21/00 ,  G02B21/16

Abstract: A device (10) for illuminating a sample (40) is described, having: at least one pulsed laser light source (12) for repeated emission of a first laser pulse along a first light path (14) and of a second laser pulse along a second light path (16) physically separated from the first light path; a superimposition element (32) for collinear superimposition of the two laser pulses in a shared light path (34); a delay stage (26) arranged in the first or the second light path (14, 16), for delaying one of the two laser pulses relative to the other laser pulse in such a way that the two laser pulses sent along the shared light path (34) onto the sample (40) exhibit a temporal superimposition; a shared chirp unit (36) arranged in the shared light path (34), for frequency-modifying influencing both of the first laser pulse and of the second laser pulse; and at least one separate chirp unit (18) arranged in the first light path (14), for frequency-modifying influencing only of the first laser pulse. The shared chirp unit (36) and the separate chirp unit (18) are coordinated with one another in order to achieve a target state. The separate chirp unit (18) is coupled to a control system (20) by which the separate chirp unit (18) is controllable with a control parameter dependent on the wavelength of the first laser pulse in order to establish the target state.

公开(公告)号：US10386303B2

公开(公告)日：2019-08-20

申请号：US15037455

申请日：2014-11-19

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Vishnu Vardhan Krishnamachari ,  Volker Seyfried ,  William C. Hay

IPC: G01N21/65 ,  G01J3/44 ,  G01J3/02 ,  G01J3/10

Abstract: A Raman microscopy imaging device (100) is described, having: a first laser light source (12) for emitting a first laser beam (16) having a first wavelength along a first light path (20); a second laser light source (44) for emitting a second laser beam (18) having a second wavelength, different from the first wavelength, along a second light path (22) physically separated from the first light path (20); a beam combining element (32) for collinearly combining the two laser beams (16, 18) in one shared light path (34) directed onto a sample; a detector (38) for sensing a measured signal on the basis of the two laser beams (16, 18) interacting with the sample; and an evaluation unit (40) for evaluating the measured signal sensed by the detector (38). According to the present invention the first laser light source (12) is embodied as a pulsed source, and the second laser light source (44) as a continuous source.