公开(公告)号：US09719855B2

公开(公告)日：2017-08-01

申请号：US14267693

申请日：2014-05-01

Applicant: LUMINEX CORPORATION

Inventor： Nicolas Arab

IPC: G01N15/00 ,  G01J3/28 ,  G01N15/14 ,  G01N21/64 ,  G01J3/42 ,  G01N15/06

CPC classification number: G01J3/2823 ,  G01J3/42 ,  G01N15/06 ,  G01N15/1463 ,  G01N21/6458 ,  G01N2015/0693

Abstract: Methods, storage mediums and systems (MS&S) are provided which successively image an imaging region of an assay analysis system (AAS) as particles are loaded into the imaging region, generate a frequency spectrum of each image via a discrete Fourier transform, integrate a same coordinate portion of each frequency spectrum and terminate the loading of particles upon computing an integral which meets preset criterion. In addition, MS&S are provided which send a signal indicative of whether enough particles are in an imaging region for further processes by an AAS based on the magnitude of integral calculated from an image's frequency spectrum. MM&S are also provided such that the steps of generating a frequency spectrum of each image and integrating a portion of each frequency spectrum are replaced by generating a convolved spatial image with a filter kernel and integrating a same coordinate portion of each convolved spatial image.

公开(公告)号：US20150044680A1

公开(公告)日：2015-02-12

申请号：US14456239

申请日：2014-08-11

Applicant: LUMINEX CORPORATION

Inventor： Doug WHITMAN ,  Nicolas Arab ,  Chuck Collins

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q2525/186 ,  C12Q2527/107 ,  C12Q2561/109 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2533/101 ,  C12Q2537/143

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of primers or probes that comprise a non-natural nucleotide linked to a reporter. Target nucleic acids are detected by the polymerization of a complementary probe or primer that incorporated a cognate non-natural nucleotide linked to a quencher.

Abstract translation: 提供了用于检测和定量核酸的方法和组合物。 在某些实施方案中，方法涉及使用包含与报告基团连接的非天然核苷酸的引物或探针。 通过掺入与猝灭剂连接的同源非天然核苷酸的互补探针或引物的聚合来检测靶核酸。

公开(公告)号：US11261481B2

公开(公告)日：2022-03-01

申请号：US16804060

申请日：2020-02-28

Applicant: LUMINEX CORPORATION

Inventor： Doug Whitman ,  Nicolas Arab ,  Chuck Collins

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6818

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of primers or probes that comprise a non-natural nucleotide linked to a reporter. Target nucleic acids are detected by the polymerization of a complementary probe or primer that incorporated a cognate non-natural nucleotide linked to a quencher.

公开(公告)号：US10733418B2

公开(公告)日：2020-08-04

申请号：US15987359

申请日：2018-05-23

Applicant: Luminex Corporation

Inventor： Matthew S. Fisher ,  Nicolas Arab

IPC: G06K9/00 ,  G01N15/10 ,  G01N15/14

Abstract: An apparatus and method for image normalization using a Gaussian residual of fit selection criteria. The method may include acquiring a two-dimensional image of a plurality of particles, where the plurality of particles comprises a plurality of calibration particles, and identifying a calibration particle by correlating a portion of the image corresponding to the calibration particle to a mathematical model (e.g. Gaussian fit). The measured intensity of the calibration particle may then be used to normalize the intensity of the image.

公开(公告)号：US20180268191A1

公开(公告)日：2018-09-20

申请号：US15987359

申请日：2018-05-23

Applicant: Luminex Corporation

Inventor： Matthew S. Fisher ,  Nicolas Arab

IPC: G06K9/00 ,  G01N15/14 ,  G01N15/10

CPC classification number: G06K9/0014 ,  G01N15/1012 ,  G01N15/1434 ,  G01N15/1456 ,  G01N15/1463 ,  G01N2015/1006 ,  G06K9/00134 ,  G06K9/00147

Abstract: An apparatus and method for image normalization using a Gaussian residual of fit selection criteria. The method may include acquiring a two-dimensional image of a plurality of particles, where the plurality of particles comprises a plurality of calibration particles, and identifying a calibration particle by correlating a portion of the image corresponding to the calibration particle to a mathematical model (e.g. Gaussian fit). The measured intensity of the calibration particle may then be used to normalize the intensity of the image.

公开(公告)号：US11098351B2

公开(公告)日：2021-08-24

申请号：US16858913

申请日：2020-04-27

Applicant: LUMINEX CORPORATION

Inventor： Nicolas Arab ,  Arnold Estrada ,  Daniel Fine ,  Ross Johnson

IPC: B01F13/00 ,  B01F3/08 ,  C12Q1/686 ,  B01L7/00 ,  B01L3/00

Abstract: Methods and devices for forming droplets are provided. In certain embodiments, the methods and devices form droplets having different diameters. In particular embodiments, the device includes a fluid supply channel, a collection chamber, and one or more nozzles disposed between the fluid supply channel and the collection chamber. Each nozzle may include an inlet portion with a channel height, a first step with a first step height and a first tread length, and a second step with a second step height and a second tread length. In certain embodiments, the first step height is greater than the channel height, and the second step height is greater than the first step height.

公开(公告)号：US10975419B2

公开(公告)日：2021-04-13

申请号：US15962077

申请日：2018-04-25

Applicant: LUMINEX CORPORATION

Inventor： Scott C. Johnson ,  Nicolas Arab ,  Doug Whitman

IPC: C12Q1/6818 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q1/6823

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.

公开(公告)号：US10752939B2

公开(公告)日：2020-08-25

申请号：US15656541

申请日：2017-07-21

Applicant: LUMINEX CORPORATION

Inventor： Scott C. Johnson ,  Nicolas Arab ,  Doug Whitman

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6823

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.

公开(公告)号：US09657332B2

公开(公告)日：2017-05-23

申请号：US14456239

申请日：2014-08-11

Applicant: LUMINEX CORPORATION

Inventor： Doug Whitman ,  Nicolas Arab ,  Chuck Collins

IPC: C12Q1/68 ,  C07H21/00

CPC classification number: C12Q1/6818 ,  C12Q2525/186 ,  C12Q2527/107 ,  C12Q2561/109 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2533/101 ,  C12Q2537/143

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of primers or probes that comprise a non-natural nucleotide linked to a reporter. Target nucleic acids are detected by the polymerization of a complementary probe or primer that incorporated a cognate non-natural nucleotide linked to a quencher.

公开(公告)号：US10662470B2

公开(公告)日：2020-05-26

申请号：US15070108

申请日：2016-03-15

Applicant: LUMINEX CORPORATION

Inventor： Nicolas Arab ,  Arnold Estrada ,  Daniel Fine ,  Ross Johnson

IPC: B01F13/00 ,  C12Q1/68 ,  C12Q1/686 ,  B01F3/08 ,  B01L7/00 ,  B01L3/00

Abstract: Methods and devices for forming droplets are provided. In certain embodiments, the methods and devices form droplets having different diameters.