公开(公告)号：US20250031690A1

公开(公告)日：2025-01-30

申请号：US18268597

申请日：2021-12-21

Applicant: LUXEMBOURG INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Elisa MOSCHINI ,  Christos SOUKOULIS ,  Tommaso SERCHI

IPC: A01N1/02 ,  C08J3/075 ,  C12N5/00

Abstract: The invention relates to an in vitro storing and/or transporting of cells, comprising: —contacting the cells with an aqueous thermo-reversible gelling composition, in a liquid form and comprising: (A) a thermo-reversible gelling polysaccharide selected from carrageenan, gellan gum and konjac gum; and (B) carboxymethyl cellulose as a thickening agent-thermo-gelling the composition by decreasing the temperature; —storing and/or transporting the in vitro cultivated cells embedded in the obtained hydrogel as obtained The invention also relates to the specific thermo-reversible gelling composition used in the process:

公开(公告)号：US20200339937A1

公开(公告)日：2020-10-29

申请号：US16954235

申请日：2018-12-31

Applicant: LUXEMBOURG INSTITUTE OF SCIENCE AND TECHNOLOGY (LIST)

Inventor： Arno GUTLEB ,  Tommaso SERCHI ,  Mathieu GERARD

IPC: C12M1/42 ,  C12M1/00 ,  C12M1/34

Abstract: A system for incubating one or more cells and/or organotypic cultures for biological investigation, in particular for toxicology assessment, comprising a bio-incubator and a pressure system fluidly connected with the bio-incubator. The pressure system is a cyclic gas pressure system configured for cyclically varying the gas pressure in the bio-incubator between a negative pressure and a positive pressure compared to the atmospheric pressure, so as to reproduce the pressure conditions in lungs of a living mammal. The system is remarkable in that the cyclic gas pressure system comprises a feedthrough with a pipe configured to deviate the air influx from the one or more cells and/or organotypic cultures.