公开(公告)号：US11156618B1

公开(公告)日：2021-10-26

申请号：US15996955

申请日：2018-06-04

Applicant: Labrador Diagnostics LLC

Inventor： Tina Noyes

IPC: G01N31/00 ,  G01N33/53 ,  G01N33/82

Abstract: Assays for rapid measurement of total vitamin D in blood are provided. Vitamin D is measured following the rapid and irreversible release of vitamin D due to denaturation and digestion of vitamin D binding proteins by aspartyl peptidases (e.g., pepsin) under acidic conditions. Such measurements may be made using a vitamin D binder (e.g., an antibody) to measure competition between free vitamin D and added, labeled vitamin D. Synergy between denaturation and degradation is believed to provide more rapid and more complete release of vitamin D than would occur with acid or enzyme alone.

公开(公告)号：US20220113322A1

公开(公告)日：2022-04-14

申请号：US17507320

申请日：2021-10-21

Applicant: Labrador Diagnostics LLC

Inventor： Tina Noyes

IPC: G01N33/82

Abstract: Assays for rapid measurement of total vitamin D in blood are provided. Vitamin D is measured following the rapid and irreversible release of vitamin D due to denaturation and digestion of vitamin D binding proteins by aspartyl peptidases (e.g., pepsin) under acidic conditions. Such measurements may be made using a vitamin D binder (e.g., an antibody) to measure competition between free vitamin D and added, labeled vitamin D. Synergy between denaturation and degradation is believed to provide more rapid and more complete release of vitamin D than would occur with acid or enzyme alone.
These measurements may be made using small amounts of whole blood, serum, or plasma, and are suitable for use in automated devices. These methods provide the advantages of reduced cost, increased speed, reduced discomfort to the subject, and increased availability and ease of use. Reagents, kits, devices, and systems for these assays are also disclosed.