公开(公告)号：US20190292310A1

公开(公告)日：2019-09-26

申请号：US16253524

申请日：2019-01-22

Applicant: NORTHEASTERN UNIVERSITY

Inventor： Ke ZHANG ,  Xuyu TAN

IPC: C08G61/08 ,  C12N15/113 ,  C12Q1/6886

Abstract: The invention provides novel synthetic methods for oligonucleotide polymerization reactions that separate the deprotection and cleavage step following solid-phase oligonucleotide synthesis into two separate steps, thereby providing fully protected hydrophobic oligonucleotides that can be further manipulated in organic solvents. The disclosed methods enable the synthesis of new structures, such as brush DNA and brush RNA polymers and micellar spherical nucleic acids (SNAs

公开(公告)号：US20220160884A1

公开(公告)日：2022-05-26

申请号：US17602307

申请日：2020-04-07

Applicant: NORTHEASTERN UNIVERSITY

Inventor： Ke ZHANG

IPC: A61K47/60 ,  A61K47/54 ,  A61K47/69 ,  A61K49/00 ,  C12N15/11

Abstract: Monodisperse structures with precise numbers of polymer arms and oligonucleotide chains conjugated to a backbone are disclosed. The structures, referred to miktoarm conjugates, are resistant to nuclease degradation and are capable of regulating gene expression in the absence of a co-carrier.

公开(公告)号：US20190292549A1

公开(公告)日：2019-09-26

申请号：US16253547

申请日：2019-01-22

Applicant: NORTHEASTERN UNIVERSITY

Inventor： Ke ZHANG ,  Dali Wang

IPC: C12N15/113 ,  C12N15/87 ,  A61K47/10 ,  A61K47/69 ,  A61K47/56

Abstract: Disclosed are bottlebrush poly(ethylene glycol) (PEG) polymer-RNA conjugates (pacRNA) having a plurality of PEG side chains attached to a polymer backbone, and one or more RNA oligonucleotides attached to the polymer backbone via a cleavable linkage. The pacRNAs offer potential useful novel RNA-based therapeutics.

公开(公告)号：US20180230467A1

公开(公告)日：2018-08-16

申请号：US15750360

申请日：2016-08-04

Applicant: NORTHEASTERN UNIVERSITY

Inventor： Ke ZHANG ,  Xueguang LU

IPC: C12N15/113 ,  C08G61/06 ,  A61K49/00 ,  C12Q1/6886

Abstract: The disclosed subject matter relates to brush polymer-oligonucleotide conjugates comprising oligonucleotides covalently attached to the backbone of a non-cationic, sterically congested brush polymer and the use of such polymer-oligonucleotide conjugates in antisense gene regulation and as diagnostic agents.

公开(公告)号：US20180369142A1

公开(公告)日：2018-12-27

申请号：US16011863

申请日：2018-06-19

Applicant: NORTHEASTERN UNIVERSITY

Inventor： Ke ZHANG ,  Dali WANG

IPC: A61K9/107 ,  B01J13/10 ,  C12N15/113

Abstract: By adjusting the spacing and length of particle surface moieties on amphiphilic micelles, hybridization kinetics, nuclease resistance, cellular uptake, and antisense efficacy of amphiphilic micelles are fine tuned.