公开(公告)号：US20210155917A1

公开(公告)日：2021-05-27

申请号：US17117870

申请日：2020-12-10

Applicant: Nanjing Tech University

Inventor： Hanjie Ying ,  Dong Liu ,  Yong Chen ,  Qingguo Liu ,  Pingkai Ouyang ,  Ming Lei ,  Huanqing Niu ,  Bin Yu ,  Di Zhang ,  Yuhan Yang ,  Shishi Wu

IPC: C12N9/52 ,  C12N1/20 ,  C12R1/15 ,  C12N15/52 ,  C12N15/77 ,  C12P13/08

Abstract: The invention discloses a recombinant Corynebacterium glutamicum for producing lysine by biofilm continuous fermentation and a construction method thereof, wherein the recombinant Corynebacterium glutamicum is constructed by overexpressing a protease gene FtsH in a Corynebacterium glutamicum; and the construction method comprises the following steps of: (1) performing PCR on a genome of the Corynebacterium glutamicum, and amplifying the FtsH gene to obtain an amplified FtsH gene segment; (2) cloning the FtsH gene segment to an overexpression plasmid to obtain a recombinant plasmid; and (3) introducing the recombinant plasmid into the Corynebacterium glutamicum, and screening to obtain the recombinant Corynebacterium glutamicum. The invention has the beneficial effects that the Corynebacterium glutamicum for overexpressing the protease FtsH is constructed in the invention, and a film-forming ability of the Corynebacterium glutamicum is enhanced, such that a yield of continuous immobilized fermentation of the Corynebacterium glutamicum is increased by 38.2% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 26.4%.

公开(公告)号：US20210147887A1

公开(公告)日：2021-05-20

申请号：US17117788

申请日：2020-12-10

Applicant: Nanjing Tech University

Inventor： Hanjie Ying ,  Dong Liu ,  Yong Chen ,  Pingkai Ouyang ,  Qingguo Liu ,  Huanqing Niu ,  Bin Yu ,  Xiwei Peng ,  Ming Lei ,  Xingyuan Cao

IPC: C12P13/08 ,  C12N9/22 ,  C12N9/14 ,  C12N11/12

Abstract: The invention discloses a method for producing lysine by utilizing adsorption and immobilized fermentation of a recombinant Corynebacterium glutamicum, wherein the recombinant Corynebacterium glutamicum is constructed by simultaneously overexpressing an adenosine triphosphate ATPase while knocking out an extracellular nuclease ExeR in a Corynebacterium glutamicum. The recombinant Corynebacterium glutamicum can effectively improve eDNA secretion of the Corynebacterium glutamicum and reduce eDNA degradation of the Corynebacterium glutamicum, so that the Corynebacterium glutamicum can be more easily adsorbed on a surface of a solid carrier for immobilized fermentation, such that a yield of continuous immobilized fermentation of the Corynebacterium glutamicum is increased by 49.67% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 29.17%.

公开(公告)号：US11098331B2

公开(公告)日：2021-08-24

申请号：US17117788

申请日：2020-12-10

Applicant: Nanjing Tech University

Inventor： Hanjie Ying ,  Dong Liu ,  Yong Chen ,  Pingkai Ouyang ,  Qingguo Liu ,  Huanqing Niu ,  Bin Yu ,  Xiwei Peng ,  Ming Lei ,  Xingyuan Cao

IPC: C12P13/08 ,  C12N11/084 ,  C12N11/096 ,  C12N9/14 ,  C12N9/22 ,  C12N11/12 ,  C12N11/14

Abstract: The invention discloses a method for producing lysine by utilizing adsorption and immobilized fermentation of a recombinant Corynebacterium glutamicum, wherein the recombinant Corynebacterium glutamicum is constructed by simultaneously overexpressing an adenosine triphosphate ATPase while knocking out an extracellular nuclease ExeR in a Corynebacterium glutamicum. The recombinant Corynebacterium glutamicum can effectively improve eDNA secretion of the Corynebacterium glutamicum and reduce eDNA degradation of the Corynebacterium glutamicum, so that the Corynebacterium glutamicum can be more easily adsorbed on a surface of a solid carrier for immobilized fermentation, such that a yield of continuous immobilized fermentation of the Corynebacterium glutamicum is increased by 49.67% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 29.17%.

公开(公告)号：US11118171B2

公开(公告)日：2021-09-14

申请号：US17117870

申请日：2020-12-10

Applicant: Nanjing Tech University

Inventor： Hanjie Ying ,  Dong Liu ,  Yong Chen ,  Qingguo Liu ,  Pingkai Ouyang ,  Ming Lei ,  Huanqing Niu ,  Bin Yu ,  Di Zhang ,  Yuhan Yang ,  Shishi Wu

IPC: C12N9/52 ,  C12N1/20 ,  C12N15/52 ,  C12N15/77 ,  C12P13/08 ,  C12R1/15

Abstract: The invention discloses a recombinant Corynebacterium glutamicum for producing lysine by biofilm continuous fermentation and a construction method thereof, wherein the recombinant Corynebacterium glutamicum is constructed by overexpressing a protease gene FtsH in a Corynebacterium glutamicum; and the construction method comprises the following steps of: (1) performing PCR on a genome of the Corynebacterium glutamicum, and amplifying the FtsH gene to obtain an amplified FtsH gene segment; (2) cloning the FtsH gene segment to an overexpression plasmid to obtain a recombinant plasmid; and (3) introducing the recombinant plasmid into the Corynebacterium glutamicum, and screening to obtain the recombinant Corynebacterium glutamicum. The invention has the beneficial effects that the Corynebacterium glutamicum for overexpressing the protease FtsH is constructed in the invention, and a film-forming ability of the Corynebacterium glutamicum is enhanced, such that a yield of continuous immobilized fermentation of the Corynebacterium glutamicum is increased by 38.2% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 26.4%.