公开(公告)号：US11098331B2

公开(公告)日：2021-08-24

申请号：US17117788

申请日：2020-12-10

Applicant: Nanjing Tech University

Inventor： Hanjie Ying ,  Dong Liu ,  Yong Chen ,  Pingkai Ouyang ,  Qingguo Liu ,  Huanqing Niu ,  Bin Yu ,  Xiwei Peng ,  Ming Lei ,  Xingyuan Cao

IPC: C12P13/08 ,  C12N11/084 ,  C12N11/096 ,  C12N9/14 ,  C12N9/22 ,  C12N11/12 ,  C12N11/14

Abstract: The invention discloses a method for producing lysine by utilizing adsorption and immobilized fermentation of a recombinant Corynebacterium glutamicum, wherein the recombinant Corynebacterium glutamicum is constructed by simultaneously overexpressing an adenosine triphosphate ATPase while knocking out an extracellular nuclease ExeR in a Corynebacterium glutamicum. The recombinant Corynebacterium glutamicum can effectively improve eDNA secretion of the Corynebacterium glutamicum and reduce eDNA degradation of the Corynebacterium glutamicum, so that the Corynebacterium glutamicum can be more easily adsorbed on a surface of a solid carrier for immobilized fermentation, such that a yield of continuous immobilized fermentation of the Corynebacterium glutamicum is increased by 49.67% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 29.17%.

公开(公告)号：US20210147887A1

公开(公告)日：2021-05-20

申请号：US17117788

申请日：2020-12-10

Applicant: Nanjing Tech University

Inventor： Hanjie Ying ,  Dong Liu ,  Yong Chen ,  Pingkai Ouyang ,  Qingguo Liu ,  Huanqing Niu ,  Bin Yu ,  Xiwei Peng ,  Ming Lei ,  Xingyuan Cao

IPC: C12P13/08 ,  C12N9/22 ,  C12N9/14 ,  C12N11/12

Abstract: The invention discloses a method for producing lysine by utilizing adsorption and immobilized fermentation of a recombinant Corynebacterium glutamicum, wherein the recombinant Corynebacterium glutamicum is constructed by simultaneously overexpressing an adenosine triphosphate ATPase while knocking out an extracellular nuclease ExeR in a Corynebacterium glutamicum. The recombinant Corynebacterium glutamicum can effectively improve eDNA secretion of the Corynebacterium glutamicum and reduce eDNA degradation of the Corynebacterium glutamicum, so that the Corynebacterium glutamicum can be more easily adsorbed on a surface of a solid carrier for immobilized fermentation, such that a yield of continuous immobilized fermentation of the Corynebacterium glutamicum is increased by 49.67% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 29.17%.