公开(公告)号：US11746029B2

公开(公告)日：2023-09-05

申请号：US16972441

申请日：2019-11-09

Applicant: NANJING UNIVERSITY

Inventor： Xuxiang Zhang ,  Depeng Wang ,  Yang He ,  Kailong Huang ,  Lin Ye ,  Bing Wu ,  Hongqiang Ren

IPC: C02F3/28 ,  C02F3/30 ,  C02F3/34 ,  C02F101/16

CPC classification number: C02F3/28 ,  C02F3/307 ,  C02F3/305 ,  C02F3/341 ,  C02F2101/16

Abstract: The present invention discloses a method for starting a single-stage system for nitrogen removal coupling partial denitrification and anammox. The two nitrogen removal processes are coupled by gradually enriching denitrifying bacteria in anammox granular sludge and then activating the partial denitrification process of the functional bacteria (denitrifying bacteria). Particularly, the method comprises: inoculating anammox granular sludge in a single-stage reactor, adding an organic carbon source in gradients to enrich denitrifying bacteria and to adapt anammox bacteria, and replacing nitrites in feed with nitrates gradually to activate the partial denitrification process.

公开(公告)号：US10557177B2

公开(公告)日：2020-02-11

申请号：US15869016

申请日：2018-01-11

Applicant: Nanjing University

Inventor： Bing Wu ,  Yue Yu ,  Huimei Chen ,  Su Liu ,  Xuxiang Zhang ,  Hongqiang Ren

IPC: C12Q1/6897 ,  C12N15/68 ,  C12Q1/02 ,  C12Q1/06 ,  C12Q1/42 ,  G01N33/18 ,  C12N15/85

Abstract: A method for characterizing toxicity of toxic pollutants and a method for characterizing comprehensive toxicity of water bodies. The methods include constructing a reporter gene cell line expressing CHOP gene associated with endoplasmic reticulum stress.

公开(公告)号：US10041118B2

公开(公告)日：2018-08-07

申请号：US14476709

申请日：2014-09-03

Applicant: Nanjing University

Inventor： Yan Zhang ,  Hongqiang Ren ,  Bing Wu ,  Xuxiang Zhang

IPC: G01N33/18 ,  C12Q1/6883 ,  G01N33/50 ,  G01N24/08 ,  G06F19/20

Abstract: A method for analyzing water toxicity, the method including: exposure experiment, sample collection, transcriptome detection, metabolome detection, screening of differentially expressed genes, screening of differentially expressed metabolites, and identification of commonly changed biological pathways in both the transcriptome and the metabolome.

公开(公告)号：US11782025B2

公开(公告)日：2023-10-10

申请号：US17219878

申请日：2021-03-31

Applicant: Nanjing University

Inventor： Haidong Hu ,  Kewei Liao ,  Jinfeng Wang ,  Bing Wu ,  Hongqiang Ren

IPC: G01N27/623 ,  G16C20/70 ,  B01D11/04 ,  G01N1/34 ,  C02F1/58 ,  C02F101/38

CPC classification number: G01N27/623 ,  B01D11/0492 ,  G01N1/34 ,  G16C20/70 ,  C02F1/58 ,  C02F2101/38 ,  C02F2209/16

Abstract: The disclosure provides a method for evaluation of removal of nitrogen-containing organic matter from the wastewater. The method includes: 1) pretreating a wastewater sample from a wastewater treatment plant; enriching nitrogen-containing organic matter in the wastewater sample with a solid-phase extraction cartridge; separating the nitrogen-containing organic matter from a substrate and disruptors of the wastewater sample, and collecting the nitrogen-containing organic matter; 2) detecting and analyzing the nitrogen-containing organic matter collected in 1) with a Fourier-transform ion cyclotron resonance mass spectrometer, thereby obtaining mass spectra of the nitrogen-containing organic matter; 3) preprocessing peak data of the mass spectra of the nitrogen-containing organic matter in each wastewater sample; setting the nitrogen-containing organic matter corresponding to the peak data as a global variable; arranging wastewater samples into cross-sectional data according to wastewater treatment processes; creating an assessment matrix for evaluating removal of the nitrogen-containing organic matter.

公开(公告)号：US11905192B2

公开(公告)日：2024-02-20

申请号：US17588274

申请日：2022-01-29

Applicant: Nanjing University

Inventor： Haidong Hu ,  Xian Cui ,  Kewei Liao ,  Lili Ding ,  Bing Wu ,  Hongqiang Ren

IPC: C02F3/00 ,  C02F3/30

CPC classification number: C02F3/006 ,  C02F3/305 ,  C02F2209/06 ,  C02F2209/08 ,  C02F2209/10 ,  C02F2209/16 ,  C02F2209/22 ,  C02F2209/44

Abstract: A method for collaborative optimization control method for organic nitrogen and inorganic nitrogen in a denitrification process is provided. The method includes: establishing ASM-mDON-DIN models for simultaneous simulation of microbial dissolved organic nitrogen (mDON) and inorganic nitrogen (DIN) in denitrification processes; and selecting a corresponding ASM-mDON-DIN model according to a set carbon/nitrogen ratio to collaboratively optimize the concentration values of mDON and DIN in the effluent in the denitrification process, to obtain best process operation parameter values.

公开(公告)号：US20220187270A1

公开(公告)日：2022-06-16

申请号：US17250222

申请日：2020-11-26

Applicant: Tongji University ,  Nanjing University

Inventor： Yinguang Chen ,  Xiong Zheng ,  Hongqiang Ren ,  Bing Wu

IPC: G01N33/18 ,  C02F1/00

Abstract: The invention relates to the technical field of environmental protection, in particular to a method for evaluating carbon source quality of a water body, an apparatus, a device and a readable storage medium. The invention provides a method for evaluating carbon source quality of a water body, including: acquiring COD and BOD5 of a first water body, wherein the first water body is a water body obtained after a water body to be measured is subjected to filtration treatment; acquiring an energy matter content in microbial cells in a second water body, wherein the second water body is a water body obtained after the first water body is subjected to anaerobic-aerobic treatment; and determining the carbon source quality of the water body to be measured according to a ratio of COD to BOD5 in the first water body and the energy matter content in the microbial cells in the second water body. The method for evaluating carbon source quality of the water body provided by the invention can effectively solve the existing problems of partial evaluation and poor pertinence of sewage biodegradability, implements accurate evaluation of the sewage carbon source on the biological nitrogen and phosphorus removal process, has the advantages of wide adaptability, accurate evaluation and the like, and has good industrialization prospects.

公开(公告)号：US20220136998A1

公开(公告)日：2022-05-05

申请号：US17219878

申请日：2021-03-31

Applicant: Nanjing University

Inventor： Haidong Hu ,  Kewei Liao ,  Jinfeng Wang ,  Bing Wu ,  Hongqiang Ren

IPC: G01N27/623 ,  G01N1/34 ,  B01D11/04 ,  G16C20/70

Abstract: The disclosure provides a method for evaluation of removal of nitrogen-containing organic matter from the wastewater. The method includes: 1) pretreating a wastewater sample from a wastewater treatment plant; enriching nitrogen-containing organic matter in the wastewater sample with a solid-phase extraction cartridge; separating the nitrogen-containing organic matter from a substrate and disruptors of the wastewater sample, and collecting the nitrogen-containing organic matter; 2) detecting and analyzing the nitrogen-containing organic matter collected in 1) with a Fourier-transform ion cyclotron resonance mass spectrometer, thereby obtaining mass spectra of the nitrogen-containing organic matter; 3) preprocessing peak data of the mass spectra of the nitrogen-containing organic matter in each wastewater sample; setting the nitrogen-containing organic matter corresponding to the peak data as a global variable; arranging wastewater samples into cross-sectional data according to wastewater treatment processes; creating an assessment matrix for evaluating removal of the nitrogen-containing organic matter.

公开(公告)号：US11299413B2

公开(公告)日：2022-04-12

申请号：US17003968

申请日：2020-08-26

Applicant: Nanjing University

Inventor： Jinfeng Wang ,  Hongqiang Ren ,  Bing Wu

IPC: C02F3/34

Abstract: A method for modifying a wastewater treatment device, the method including: 1) fixing a filling support in a wastewater pool, where the filling support includes a support frame; the support frame includes a hollow shaft, a fixed ring, and at least one vane; 2) adjusting the height of the filling support, until the fixed ring is submerged below the liquid level of the wastewater pool, where the water intake velocity of the wastewater pool is no less than 0.25 m/s, whereby a water current is produced and drives the support frame to rotate; 3) adding a functional liquid into the hollow shaft; and 4) adding an enzyme mixture to the hollow shaft when the rotation speed of the support frame is less than a first preset value, until the rotation speed of the support frame reaches a second preset value.

公开(公告)号：US12111288B2

公开(公告)日：2024-10-08

申请号：US17732574

申请日：2022-04-29

Applicant: Nanjing University

Inventor： Jinfeng Wang ,  Deyuan Dong ,  Haidong Hu ,  Bing Wu ,  Hongqiang Ren

IPC: G01N29/02 ,  G01N1/14 ,  G01N1/38

CPC classification number: G01N29/02 ,  G01N1/14 ,  G01N1/38 ,  G01N2291/02416

Abstract: A device for in-situ measuring settleability of activated sludge includes a sample chamber, an ultrasonic time domain reflectometer, a magnetic stirrer, an ultrasonic probe, a sample inlet, a sample outlet, and a stagnant zone. The sample chamber is configured to hold an activated sludge sample, and the ultrasonic time domain reflectometer is configured to measure the settling characteristics of the activated sludge. The stagnant zone is disposed in the sample chamber. The sample inlet and the sample outlet communicate with the sample chamber. The stagnant zone includes a top part, a sidewall, and a bottom part. The sample inlet is connected to the top part of the stagnant zone. The sample outlet is connected to the sidewall of the stagnant zone. The magnetic stirrer is disposed at the bottom part of the stagnant zone. The ultrasonic probe is disposed on the top part of the stagnant zone.

公开(公告)号：US11866355B2

公开(公告)日：2024-01-09

申请号：US17343711

申请日：2021-06-09

Applicant: Nanjing University

Inventor： Haidong Hu ,  Kewei Liao ,  Sijia Ma ,  Yuanji Shi ,  Hongqiang Ren ,  Xuxiang Zhang ,  Bing Wu ,  Jinfeng Wang

IPC: C02F3/12 ,  C02F11/04 ,  C02F1/32 ,  C02F11/12 ,  C02F101/16 ,  C02F1/00

CPC classification number: C02F3/1226 ,  C02F1/325 ,  C02F3/121 ,  C02F3/1215 ,  C02F11/04 ,  C02F11/12 ,  C02F1/32 ,  C02F2001/007 ,  C02F2101/16 ,  C02F2201/322 ,  C02F2209/02 ,  C02F2209/06 ,  Y02W10/10

Abstract: A system for cleaning wastewater, includes: an absorption-biodegradation-denitrification (ABN) reactor, a sequential adsorption reactor, a disinfection reactor, and a sludge anaerobic fermentation reactor. The ABN reactor is an integrated reactor including: a biosorption tank, an intermediate sedimentation tank, a biologically-enhanced degradation tank, a denitrification biofilter, and a secondary sedimentation tank. The pretreated wastewater is introduced into the ABN reactor for removal of chemical oxygen demand, nitrogen and phosphorus; the ABN reactor effluent is introduced into the sequential adsorption reactor for the removal of high-risk pollutants; the sequential adsorption reactor effluent is introduced into the disinfection reactor for the elimination of viruses and other pathogenic microorganisms; the sludge produced by the ABN reactor is introduced into the anaerobic sludge fermentation reactor for alkaline fermentation. The system is effective for removing high-risk pollutants and reducing effluent toxicity, which can be used for the upgrading and reconstruction of the wastewater treatment system.