公开(公告)号：US20190211297A1

公开(公告)日：2019-07-11

申请号：US16326826

申请日：2016-10-14

Applicant: Ni HUI

Inventor： Ni HUI ,  Shiyao HUI ,  Ii LI

IPC: C12M1/12 ,  C12M1/00 ,  C12N5/00 ,  B01J8/00 ,  B01J8/02

CPC classification number: C12M25/20 ,  B01J8/003 ,  B01J8/0228 ,  B01J8/0285 ,  C12M29/02 ,  C12M29/06 ,  C12N5/0075

Abstract: A large circulating fluidized bed cell bioreactor and a method for culturing animal cells. The reactor comprises an agitator tank body (01), an agitator tank base (50), a water inlet silicone hose (06), a backflow silicone hose (08), a cell culture tank (10), a culture tank base (40), a backflow pipe (15), a water inlet pipe (12), an agitator (20), a reactor tray (30), and a tube support plate (60). By means of cooperation among the above components, defects and shortcomings of fluidized bed cell bioreactors in the prior art including proneness to microbial contamination, a low level of dissolved oxygen, and a low working volume, are effectively resolved, advantageous in the popularization and application of this technology.