公开(公告)号：US20210140881A1

公开(公告)日：2021-05-13

申请号：US17094901

申请日：2020-11-11

Applicant: Nirrin Technologies, Inc.

Inventor： Bryan A. Hassell ,  Walid A. Atia ,  David P. Marchessault

IPC: G01N21/39 ,  C12M1/34 ,  G01N33/68 ,  G01N21/45 ,  G01N21/359 ,  G01N21/3577

Abstract: A method for studying cell viability and protein aggregation involves establishing a Fabry Perot etalon signal within an optical spectroscopic feature, e.g., in the near infrared region. Protein aggregation and cell viability can be reflected by changes observed in the magnitude of the Fourier Transform peaks observed in the frequency or space domain associated with the contrast of the etalon. In short, the presence of viable cells and protein aggregates can degrade the etalon contrast of an etalon window. In some cases, the concentration of cells and monomeric protein can be measured as well.

公开(公告)号：US12031908B2

公开(公告)日：2024-07-09

申请号：US17094901

申请日：2020-11-11

Applicant: Nirrin Technologies, Inc.

Inventor： Bryan A. Hassell ,  Walid A. Atia ,  David P. Marchessault

IPC: B01L3/00 ,  B01F23/00 ,  B01F23/41 ,  B01F101/23 ,  B23Q17/24 ,  C12M1/34 ,  C12Q1/18 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/39 ,  G01N21/45 ,  G01N21/64 ,  G01N33/50 ,  G01N33/68

CPC classification number: G01N21/39 ,  C12M41/32 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/45 ,  G01N33/6854 ,  G01N2201/06113

Abstract: A method for studying cell viability and protein aggregation involves establishing a Fabry Perot etalon signal within an optical spectroscopic feature, e.g., in the near infrared region. Protein aggregation and cell viability can be reflected by changes observed in the magnitude of the Fourier Transform peaks observed in the frequency or space domain associated with the contrast of the etalon. In short, the presence of viable cells and protein aggregates can degrade the etalon contrast of an etalon window. In some cases, the concentration of cells and monomeric protein can be measured as well.

公开(公告)号：US20230034379A1

公开(公告)日：2023-02-02

申请号：US17965017

申请日：2022-10-13

Applicant: Nirrin Technologies, Inc.

Inventor： Bryan A. Hassell ,  David P. Marchessault ,  Christopher R. Saulnier ,  John C. Ho ,  Walid A. Atia

IPC: G01N15/02 ,  G01N15/14 ,  G01N21/53

Abstract: A device for monitoring a bioreactor is configured for in-situ analysis, e.g., by NIR, without the need for withdrawing a sample into a sample cell or into an ex-situ arrangement. The device can be inserted into a port of the bioreactor and provides a sample detection region defined by an optical element such as a lens and a photodetector. The electrical signal obtained from a photodetector that is part of the device can be directed to an analyzer via a detachable electrical connection.

公开(公告)号：US20220298467A1

公开(公告)日：2022-09-22

申请号：US17696635

申请日：2022-03-16

Applicant: Nirrin Technologies, Inc.

Inventor： Bryan A. Hassell ,  David P. Marchessault

IPC: C12M1/34 ,  C12M1/00 ,  C12M3/06 ,  G01N21/359 ,  G01N21/39

Abstract: A process conducted in a rocking bioreactor is monitored using a flexible bag fitted with an in-situ probe. The probe includes a disposable bag insert or patch and a reusable probe module. The patch is secured to a wall of the bag, and the probe module is a detachable part that can be mated to the bag patch, then disassembled for future use, for example. The patch is secured to the bag, defining a sample gap, and the probe module is inserted or mated with the patch. The probe module includes one or more source elements and one or more detector element. Light is transmitted from the source elements, through the sample gap, to the detector element(s), which detect the light after interaction with contents of the bag.

公开(公告)号：US20250020576A1

公开(公告)日：2025-01-16

申请号：US18767034

申请日：2024-07-09

Applicant: Nirrin Technologies, Inc.

Inventor： Bryan A. Hassell ,  David P. Marchessault

IPC: G01N21/05 ,  G01N21/35 ,  G01N21/39

Abstract: A system for analyzing a sample, e.g., by NIR, includes a clamshell apparatus and a tunable laser. The apparatus has a fixed first section and a second section which can move between an open position and a closed position. A sample detection region is formed between an input rod and an output rod by lowering the second section to the closed position. A flowcell inserted between these sections can be used to analyze flowing samples. One illustrative arrangement employs a reference detector positioned in the first section and a sample detector positioned in the second section.

公开(公告)号：US20240117293A1

公开(公告)日：2024-04-11

申请号：US18483882

申请日：2023-10-10

Applicant: NIRRIN TECHNOLOGIES, INC.

Inventor： Bryan A. Hassell ,  Gregory A. Phelps ,  David P. Marchessault

IPC: C12M1/00 ,  G01N21/27

CPC classification number: C12M41/06 ,  G01N21/274 ,  G01N2201/06113 ,  G01N2201/0683

Abstract: Devices for monitoring a bioreactor are configured for in-situ or ex-situ analysis. Noise from polarization dependent loss is addressed with a polarizer and reference photodetector for detecting a swept wavelength signal from a tunable laser spectrometer after transmission on a optical fiber but before a sample detection region.

公开(公告)号：US20230060089A1

公开(公告)日：2023-02-23

申请号：US17893279

申请日：2022-08-23

Applicant: Nirrin Technologies, Inc.

Inventor： Bryan A. Hassell ,  Gregory A. Phelps ,  David P. Marchessault

IPC: G01N15/14 ,  C12M1/34

Abstract: A method and system for studying cell viability and protein aggregation. In one aspect, the method relies on speckle information to analyze cells or other structures present in a fluid. A probe for measuring in situ structures includes a tip section having a sample detection region and a camera provided with image sensors.

公开(公告)号：US20210371785A1

公开(公告)日：2021-12-02

申请号：US17337055

申请日：2021-06-02

Applicant: Nirrin Technologies, Inc.

Inventor： Bryan A. Hassell ,  David P. Marchessault

IPC: C12M1/00 ,  C12M1/34 ,  C12M1/36 ,  C12M3/04

Abstract: An in situ probe, comprising reusable and disposable components, can be employed to measure cell viability in a rocking bioreactor.

公开(公告)号：US20240319090A1

公开(公告)日：2024-09-26

申请号：US18733911

申请日：2024-06-05

Applicant: Nirrin Technologies, Inc.

Inventor： Bryan A. Hassell ,  Walid A. Atia ,  David P. Marchessault

IPC: G01N21/39 ,  C12M1/34 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/45 ,  G01N33/68

CPC classification number: G01N21/39 ,  C12M41/32 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/45 ,  G01N33/6854 ,  G01N2201/06113

Abstract: A method for studying cell viability and protein aggregation involves establishing a Fabry Perot etalon signal within an optical spectroscopic feature, e.g., in the near infrared region. Protein aggregation and cell viability can be reflected by changes observed in the magnitude of the Fourier Transform peaks observed in the frequency or space domain associated with the contrast of the etalon. In short, the presence of viable cells and protein aggregates can degrade the etalon contrast of an etalon window. In some cases, the concentration of cells and monomeric protein can be measured as well.

公开(公告)号：US11499903B2

公开(公告)日：2022-11-15

申请号：US17030032

申请日：2020-09-23

Applicant: Nirrin Technologies, Inc.

Inventor： Bryan A. Hassell ,  David P. Marchessault ,  Christopher R. Saulnier ,  John C. Ho ,  Walid A. Atia

IPC: G01N15/02 ,  G01N15/14 ,  G01N21/53 ,  G01N15/10

Abstract: A device for monitoring a bioreactor is configured for in-situ analysis, e.g., by NIR, without the need for withdrawing a sample into a sample cell or into an ex-situ arrangement. The device can be inserted into a port of the bioreactor and provides a sample detection region defined by an optical element such as a lens and a photodetector. The electrical signal obtained from a photodetector that is part of the device can be directed to an analyzer via a detachable electrical connection.