公开(公告)号：US20240402156A1

公开(公告)日：2024-12-05

申请号：US18262659

申请日：2022-01-25

Applicant: Northeastern University

Inventor： Meni Wanunu ,  Sara Rouhanifard

IPC: G01N33/50 ,  B01L3/00 ,  C12M1/34

Abstract: A system and method for assessing properties of particles, the system comprising: a first structure defining an interior cavity, configured to contain an interior cavity fluid, and a barrier separating the interior cavity from an exterior cavity configured to contain an exterior cavity fluid, the barrier defining a nanopore therethrough fluidically coupling the interior and exterior cavities, the first structure further comprising at least one feature configured to interface with at least one complementary feature of a second structure that defines the exterior cavity; and electrodes configured to energize and apply a voltage gradient across the nanopore between the interior cavity and exterior cavity, the voltage gradient of sufficient magnitude to induce particles to migrate via the nanopore between the interior cavity and the exterior cavity and produce an electronic signature representative of at least one property of the particles.

公开(公告)号：US11703476B2

公开(公告)日：2023-07-18

申请号：US17083264

申请日：2020-10-28

Applicant: Northeastern University

Inventor： Meni Wanunu ,  Mohammadamin Alibakhshi ,  Xinqi Kang ,  Zhuoyu Zhang

IPC: G01N27/416 ,  B01L3/00 ,  G01N33/487

CPC classification number: G01N27/4166 ,  B01L3/50273 ,  B01L3/502707 ,  B01L3/502715 ,  G01N33/48721 ,  B01L2300/0645 ,  B01L2300/0832 ,  B01L2400/0406 ,  B01L2400/0415

Abstract: An apparatus is provided for sensing a molecule in a sample. The apparatus utilizes an electric field to draw molecules from a first chamber through an aperture, defined by a chemical layer, into a second chamber. The apparatus can detect a DNA molecule with, for example, 4, 5, or 6 unique base pairs. As molecules pass through the aperture, a sensor detects or measures a change in an electric parameter used to generate the electric field, thereafter translating the change in the electric parameter into information about the molecule. A divider element separates the first and second chambers and supports a chemical layer defining the aperture. The apparatus enables detection or measurement of molecules over prolonged time at a higher electric field strength than other nanopores, due to a combination of the shape of the divider, structural elements thereon, and thickness of the chemical layer at the aperture.

公开(公告)号：US20220334097A1

公开(公告)日：2022-10-20

申请号：US17754114

申请日：2020-09-25

Applicant: Northeastern University

Inventor： Meni Wanunu ,  Mohammadamin Alibakhshi ,  Fatemeh Farhangdoust

IPC: G01N33/487 ,  G01N21/64

Abstract: A system and apparatus are provided for sensing a molecule in a sample for identifying the molecule in the sample. Also provided is a method of manufacturing an apparatus for sensing a molecule in a sample. The system and apparatus may contain a composition of a mixture of a buffer solution and a sample solution containing a sample. The apparatus contains a series of wells. The sample is deposited into the system and a molecule having an electric charge in the sample attaches to a layer in a well of the apparatus, allowing for a sensor connected to the apparatus to identify the molecule.

公开(公告)号：US11994508B2

公开(公告)日：2024-05-28

申请号：US17645943

申请日：2021-12-23

Applicant: Northeastern University ,  The Board of Trustees of the University of Illinois

Inventor： Meni Wanunu ,  Luning Yu ,  Aleksei Aksimentiev ,  Behzad Mehrafrooz

IPC: G01N33/487 ,  B01L3/00 ,  C12Q1/34 ,  G01N33/68

CPC classification number: G01N33/48721 ,  B01L3/502715 ,  B01L3/502761 ,  C12Q1/34 ,  G01N33/6818 ,  B01L2200/0663 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2400/0418

Abstract: A method and system for performing single molecule proteomics utilizing a nanopore sensor to measure an electronic signature of protein or peptide being transported through the nanopore from a first chamber to a second chamber. The protein's electronic signature is a function of ionic current over time. The method and system utilizing an agent, such as guanidinium chloride, to bind to the nanopore's interior and provide an electroosmotic force within the nanopore. The electroosmotic force, in some embodiments, enables stretching and unfolding of the protein during transport through the nanopore. The agent may also or alternatively induce the unfolding of the protein before transport through the nanopore and/or provide force moving the protein through the nanopore.

公开(公告)号：US11933778B2

公开(公告)日：2024-03-19

申请号：US17809705

申请日：2022-06-29

Applicant: Northeastern University ,  University of York

Inventor： Meni Wanunu ,  Alfred Antson ,  Sandra Greive ,  Benjamin Cressiot

IPC: G01N33/487 ,  B81B1/00 ,  B82Y15/00 ,  C07K14/005 ,  C12Q1/6869

CPC classification number: G01N33/48721 ,  B81B1/002 ,  C07K14/005 ,  C12Q1/6869 ,  B82Y15/00 ,  C12N2795/00022 ,  Y10S930/22

Abstract: Hybrid nanopores, comprising a protein pore supported within a solid-state membrane, which combine the robust nature of solid-state membranes with the easily tunable and precise engineering of protein nanopores. In an embodiment, a lipid-free hybrid nanopore comprises a water soluble and stable, modified portal protein of the Thermus thermophilus bacteriophage G20c, electrokinetically inserted into a larger nanopore in a solid-state membrane. The hybrid pore is stable and easy to fabricate, and exhibits low peripheral leakage, allowing sensing and discrimination among different types of biomolecules.

公开(公告)号：US20220373533A1

公开(公告)日：2022-11-24

申请号：US17809705

申请日：2022-06-29

Applicant: Northeastern University ,  University of York

Inventor： Meni Wanunu ,  Alfred Antson ,  Sandra Greive ,  Benjamin Cressiot

IPC: G01N33/487 ,  C12Q1/6869 ,  C07K14/005 ,  B81B1/00

Abstract: Hybrid nanopores, comprising a protein pore supported within a solid-state membrane, which combine the robust nature of solid-state membranes with the easily tunable and precise engineering of protein nanopores. In an embodiment, a lipid-free hybrid nanopore comprises a water soluble and stable, modified portal protein of the Thermus thermophilus bacteriophage G20c, electrokinetically inserted into a larger nanopore in a solid-state membrane. The hybrid pore is stable and easy to fabricate, and exhibits low peripheral leakage, allowing sensing and discrimination among different types of biomolecules.

公开(公告)号：US20210123884A1

公开(公告)日：2021-04-29

申请号：US17083264

申请日：2020-10-28

Applicant: Northeastern University

Inventor： Meni Wanunu ,  Mohammadamin Alibakhshi ,  Xinqi Kang ,  Zhuoyu Zhang

IPC: G01N27/416 ,  B01L3/00 ,  G01N33/487

Abstract: An apparatus is provided for sensing a molecule in a sample. The apparatus utilizes an electric field to draw molecules from a first chamber through an aperture, defined by a chemical layer, into a second chamber. The apparatus can detect a DNA molecule with, for example, 4, 5, or 6 unique base pairs. As molecules pass through the aperture, a sensor detects or measures a change in an electric parameter used to generate the electric field, thereafter translating the change in the electric parameter into information about the molecule. A divider element separates the first and second chambers and supports a chemical layer defining the aperture. The apparatus enables detection or measurement of molecules over prolonged time at a higher electric field strength than other nanopores, due to a combination of the shape of the divider, structural elements thereon, and thickness of the chemical layer at the aperture.

公开(公告)号：US10047392B2

公开(公告)日：2018-08-14

申请号：US15119859

申请日：2015-02-23

Applicant: Northeastern University

Inventor： Andrey Ivankin ,  Joseph Larkin ,  Robert Henley ,  Meni Wanunu

IPC: C12Q1/6869 ,  G01N21/64 ,  G01N33/487 ,  G01N21/77

Abstract: Described herein are systems for analysis of biopolymers and complexes containing biopolymers based on optical measurement of ion flux through pores. Also described are methods of using such devices for analysis of biopolymers and complexes containing biopolymers, including methods of determining the nucleotide sequences of polynucleotides.

公开(公告)号：US20230017101A1

公开(公告)日：2023-01-19

申请号：US17779995

申请日：2020-11-27

Applicant: Northeastern University

Inventor： Mohammadamin Alibakhshi ,  Meni Wanunu ,  Xinqi Kang

IPC: B01D67/00

Abstract: A method for manufacturing a component and a component are provided for sensing a molecule. The method includes controlling a temperature during a reaction of two gases that react to produce a crystalline film spanning at least a cross-sectional area of a nanoaperture defined by a substrate among an array of nanoapertures aligned with crater structures defined by the substrate. A unique chemical vapor deposition (CVD) method that introduces a first gas and a second gas allows for formation of the crystalline film. When used in a molecule sensor, the component enables a user to record double-stranded DNA (dsDNA) translocations at unprecedented high (e.g., 1 MHz) bandwidths. The method for manufacturing the component enables development of applications requiring single-layer membranes built at- scale and enables high throughput 2-dimensional (2D) nanofluidics and nanopore studies.

公开(公告)号：US11408880B2

公开(公告)日：2022-08-09

申请号：US16416139

申请日：2019-05-17

Applicant: Northeastern University ,  University of York

Inventor： Meni Wanunu ,  Alfred Antson ,  Sandra Greive ,  Benjamin Cressiot

IPC: G01N33/487 ,  C12Q1/6869 ,  C07K14/005 ,  B81B1/00 ,  B82Y15/00

Abstract: Hybrid nanopores, comprising a protein pore supported within a solid-state membrane, which combine the robust nature of solid-state membranes with the easily tunable and precise engineering of protein nanopores. In an embodiment, a lipid-free hybrid nanopore comprises a water soluble and stable, modified portal protein of the Thermus thermophilus bacteriophage G20c, electrokinetically inserted into a larger nanopore in a solid-state membrane. The hybrid pore is stable and easy to fabricate, and exhibits low peripheral leakage, allowing sensing and discrimination among different types of biomolecules.