公开(公告)号：US20220244265A1

公开(公告)日：2022-08-04

申请号：US17612713

申请日：2020-05-22

Applicant: Northeastern University

Inventor： Pushkar Kulkarni ,  Roger W. Giese ,  Poguang Wang

IPC: G01N33/58

Abstract: The disclosure relates to a diazirine precursor mass tag compound represented by structural formula (I) Also disclosed is a method for detecting analytes in a sample, comprising derivatizing the analytes with the compound of formula (I), and detecting the resulting derivatized analytes by a mass or ion mobility spectrometry.

公开(公告)号：US11866779B2

公开(公告)日：2024-01-09

申请号：US16961074

申请日：2019-02-28

Applicant: Northeastern University

Inventor： Roger W. Giese ,  Poguang Wang

IPC: C12Q1/68 ,  C12Q1/6872

CPC classification number: C12Q1/6872 ,  C12Q2523/313

Abstract: MALDI-MS operated slightly under matrix storm conditions with a high-energy-transfer, acidic matrix, or these conditions with a high sample to matrix ratio, can with ultrasensitivity detect a nucleobase, modified or canonical, of a nucleic acid species as a jettisoned, protonated molecule.

公开(公告)号：US11454628B2

公开(公告)日：2022-09-27

申请号：US16433617

申请日：2019-06-06

Applicant: Northeastern University

Inventor： Pushkar Kulkarni ,  Poguang Wang ,  Roger W. Giese

IPC: G01N33/543 ,  G01N1/40 ,  G01N1/44 ,  G01N33/02 ,  G01N33/15 ,  G01N1/28 ,  G01N1/22

Abstract: The present disclosure relates to a method for detecting a compound, comprising the steps of: contacting a compound with a solid analytical surface (SAS), thereby forming an SAS with an absorbed compound; contacting the SAS with the absorbed compound with a mass tag, wherein the mass tag reacts with the absorbed compound, thereby forming an SAS with a covalently mass-tagged absorbed compound; and detecting the covalently mass-tagged absorbed compound by mass spectrometry. Also disclosed is a device for collecting breath aerosol, comprising a card or an envelope, wherein the card or the envelope comprise a tab, wherein the tab is a SAS.

公开(公告)号：US20210062257A1

公开(公告)日：2021-03-04

申请号：US16961074

申请日：2019-02-28

Applicant: Northeastern University

Inventor： Roger W. Giese ,  Poguang Wang

IPC: C12Q1/6872

Abstract: MALDI-MS operated slightly under matrix storm conditions with a high-energy-transfer, acidic matrix, or these conditions with a high sample to matrix ratio, can with ultrasensitivity detect a nucleobase, modified or canonical, of a nucleic acid species as a jettisoned, protonated molecule.

公开(公告)号：US20200041500A1

公开(公告)日：2020-02-06

申请号：US16433617

申请日：2019-06-06

Applicant: Northeastern University

Inventor： Pushkar Kulkarni ,  Poguang Wang ,  Roger W. Giese

IPC: G01N33/543 ,  G01N1/28 ,  G01N1/40 ,  G01N1/44 ,  G01N33/02 ,  G01N33/15

Abstract: The present disclosure relates to a method for detecting a compound, comprising the steps of: contacting a compound with a solid analytical surface (SAS), thereby forming an SAS with an absorbed compound; contacting the SAS with the absorbed compound with a mass tag, wherein the mass tag reacts with the absorbed compound, thereby forming an SAS with a covalently mass-tagged absorbed compound; and detecting the covalently mass-tagged absorbed compound by mass spectrometry. Also disclosed is a device for collecting breath aerosol, comprising a card or an envelope, wherein the card or the envelope comprise a tab, wherein the tab is a SAS.

公开(公告)号：US20210340603A1

公开(公告)日：2021-11-04

申请号：US17307607

申请日：2021-05-04

Applicant: Northeastern University

Inventor： Roger W. Giese ,  Poguang Wang

IPC: C12Q1/6827 ,  C12Q1/6834

Abstract: Disclosed is a method for detecting the presence of a DNA adduct in DNA. The method involves the pre-labeling of an adducted nucleotide in the DNA with a quaternary ammonium compound.

公开(公告)号：US20180242884A1

公开(公告)日：2018-08-30

申请号：US15897602

申请日：2018-02-15

Applicant: Northeastern University

Inventor： Pushkar Kulkarni ,  Poguang Wang ,  Roger W. Giese

IPC: A61B5/097 ,  G01N33/497 ,  A61B5/08

CPC classification number: A61B5/097 ,  A61B5/082 ,  A61B5/6803 ,  A61B10/00 ,  A61B2010/0087 ,  G01N33/497 ,  G01N2033/4975

Abstract: Disclosed are devices comprising a porous sorptive tab and a substructure, such as a face mask, for collecting breath aerosol from a subject. The device is configured such that, in use, a subject breathes onto or through the sorptive tab. Also disclosed are specific methods of analyzing breath aerosol from a subject.

公开(公告)号：US10036743B2

公开(公告)日：2018-07-31

申请号：US15547588

申请日：2016-02-01

Applicant: NORTHEASTERN UNIVERSITY

Inventor： Roger Giese ,  Poguang Wang

IPC: G01N33/52 ,  C07C217/48 ,  C07C217/58

CPC classification number: G01N33/52 ,  C07C217/48 ,  C07C217/58 ,  G01N2458/15 ,  G01N2560/00

Abstract: The invention provides methods of detecting an analyte by multi-stage mass spectrometry with improved S/N ratio. An analyte is labeled with a positively-charged mass tag to form a precursor ion that leads by anchimeric assistance to a greatly enhanced, analyte-characteristic first product ion that can, in turn, lead to a greatly enhanced, analyte-characteristic second product ion in a mass spectrometer. Either a three stage mass spectrometer (true MS3) or a two-stage mass spectrometer (MS2) operated in a pseudo MS3 mode can be used. The precursor ion is split via an anchimeric-assisted reaction to form a first product ion, which in turn can be fragmented to form the second product ion. The methods offer extreme ultrasensitivity, at the low amol level. The invention also provides anchimeric mass tags for use in the methods. A wide variety of previously undetectable analytes of biological or environmental origin can be detected and quantified.