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    TEMPERATURE-SENSITIVE CAS9 PROTEIN
    1.
    发明申请
    TEMPERATURE-SENSITIVE CAS9 PROTEIN 审中-公开

    公开(公告)号：US20200318086A1

    公开(公告)日：2020-10-08

    申请号：US16652920

    申请日：2018-11-07

    Applicant: Novozymes A/S

    Inventor： Carsten Anderson ,  Poul Erik Pedersen ,  Michael Dolberg Rasmussen ,  Carsten Hjort

    IPC: C12N9/22 ,  C12N15/63

    Abstract: The present invention relates to temperature-sensitive variants of a Class-II Cas9 protein, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising polynucleotides encoding said variants, host cells expressing said variant, methods of transient repression and expression of one or more genome target sequence using said variants, and use of said variants, polynucleotides, nucleic acid constructs, expression vectors, host cells, and methods.

    Stably maintained multiple copies of at least two ORF in the same orientation
    6.
    发明申请
    Stably maintained multiple copies of at least two ORF in the same orientation 审中-公开
    以相同的方向稳定地保持至少两个ORF的多个拷贝

    公开(公告)号：US20140106457A1

    公开(公告)日：2014-04-17

    申请号：US14034124

    申请日：2013-09-23

    Applicant: Novozymes A/S

    Inventor： Steen Troels Jorgensen ,  Poul Erik Pedersen

    IPC: C12N9/52

    CPC classification number: C12N9/52 ,  C12N15/67 ,  C12N15/902 ,  C12N2800/40 ,  C12N2830/20

    Abstract: The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of: a) providing the at least two ORF's encoding the same polypeptide, wherein the at least two DNA sequences of the ORF's differ in at least one position; b) integrating the at least two ORF's in the same orientation on the host cell chromosome.

    Abstract translation: 本发明涉及构建表达目标多肽的宿主细胞的方法，其至少两个稳定整合到宿主细胞的染色体上的ORF包括以下步骤：a）提供编码相同多肽的至少两个ORF，其中 ORF的至少两个DNA序列在至少一个位置上不同; b）在宿主细胞染色体上以相同方向整合至少两个ORF。

    Subtilases
    8.
    发明申请
    Subtilases 审中-公开
    分散液

    公开(公告)号：US20140127782A1

    公开(公告)日：2014-05-08

    申请号：US14150378

    申请日：2014-01-08

    Applicant: Novozymes A/S

    Inventor： Preben Nielsen ,  Poul Erik Pedersen ,  Helle Outtrup

    IPC: C12N9/50

    CPC classification number: C12N9/50 ,  C12N9/54 ,  C12N15/52 ,  C12N15/62

    Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.

    Abstract translation: 本发明涉及来自野生型细菌的新型JP170类枯草芽孢杆菌，其杂种，以及构建和生产这些蛋白酶的方法。 此外，本发明涉及所要求的残留物在洗涤剂如衣物或自动餐具洗涤剂中的用途。

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