公开(公告)号：US20160305889A1

公开(公告)日：2016-10-20

申请号：US15030183

申请日：2014-10-20

Applicant: OSAKA UNIVERSITY

Inventor： Hiroyuki YOSHIKAWA ,  Eiichi TAMIYA ,  Shuhei IMURA

IPC: G01N21/82 ,  G01N21/78 ,  C12Q1/26

CPC classification number: G01N21/82 ,  C12Q1/26 ,  G01N21/78 ,  G01N33/535 ,  G01N33/581 ,  G01N2021/7773 ,  G01N2201/06113 ,  G01N2201/062 ,  G01N2333/904

Abstract: The present invention provide a method for detecting a test substance utilizing a color development caused by an enzyme reaction, or a color development caused by a specific interaction such as an antigen-antibody reaction and an enzyme reaction, the test substance being detected rapidly, sensitively and quantitatively without using a spectroscopic measurement device. The method for measuring a concentration of a test substance by the present invention comprises the steps of: generating a peroxide from the test substance; obtaining a polymerized substance by bringing an oxidoreductase for producing a polymerized substance and a substrate of the oxidoreductase for producing a polymerized substance into contact with the peroxide; and irradiating the polymerized substance with light to record a temporal variation information of an intensity of scattered light generated from an irradiation point.

Abstract translation: 本发明提供一种利用酶反应引起的显色或由抗原 - 抗体反应和酶反应等特定相互作用引起的显色检测检测物质的方法，检测物质被快速检测 并且定量地不使用光谱测量装置。 通过本发明测定被检物质的浓度的方法包括以下步骤：从测试物质产生过氧化物; 通过使生成聚合物质的氧化还原酶和用于制备聚合物质的氧化还原酶的底物与过氧化物接触来获得聚合物质; 并用光照射聚合物，记录从照射点产生的散射光的强度的时间变化信息。