公开(公告)号：US20030104428A1

公开(公告)日：2003-06-05

申请号：US10177062

申请日：2002-06-21

Applicant: President and Fellows of Harvard College

Inventor： Daniel Branton ,  Stephen S. Laderman ,  Zohar Yakhini ,  Jeffrey R. Sampson ,  Hui Wang

IPC: C12Q001/68 ,  C07H021/04 ,  C12P019/34

CPC classification number: C12Q1/68 ,  C12Q1/6813 ,  G01N33/48721 ,  C12Q2565/631 ,  C12Q2537/143

Abstract: To probe the characteristics of a target nucleic acid molecule or a population of target nucleic acid molecules, a local cross-sectional area, local charge, or local chemistry of a molecule is modified. The modified nucleic acid is contacted with a substrate that includes a detector that is responsive to the modification in the local cross-sectional area, local charge, or local chemistry of the nucleic acid molecule. The modified nucleic acid molecule traverses a defined and preferably molecular dimensioned volume on the substrate so that nucleotides of the modified nucleic acid molecule interact with the detector in sequential order, whereby data correlating with the cross-sectional area, local charge, or local chemistry of the nucleic acid molecule are obtained. The nucleic acid molecule may be modified in a number of locations along the molecule's length. Because the individual nucleotides of the nucleic acid molecule interact with the detector in sequential order, information regarding the location and composition of a plurality of modified sites along a single molecule can be obtained.

Abstract translation: 为了探测靶核酸分子或靶核酸分子群的特征，修饰了分子的局部横截面积，局部电荷或局部化学。 经修饰的核酸与包含检测器的底物接触，所述检测器响应于核酸分子的局部横截面积，局部电荷或局部化学物质的修饰。 修饰的核酸分子穿过基底上限定且优选分子尺寸的体积，使得修饰的核酸分子的核苷酸以检测器的顺序相互作用，由此与横截面积，局部电荷或局部化学相关的数据 得到核酸分子。 核酸分子可以沿着分子的长度在多个位置修饰。 因为核酸分子的各个核苷酸以连续的顺序与检测器相互作用，所以可以获得关于沿单个分子的多个修饰位点的位置和组成的信息。