公开(公告)号：US10274461B2

公开(公告)日：2019-04-30

申请号：US15621271

申请日：2017-06-13

Applicant: The Trustees of Princeton University

Inventor： Robert H. Austin ,  Zhaoning Yu ,  Jonas O. Tagenfeldt ,  Stephen Y. Chou ,  Han Cao

IPC: G01N27/447

Abstract: Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.

公开(公告)号：US20190285582A1

公开(公告)日：2019-09-19

申请号：US16367701

申请日：2019-03-28

Applicant: The Trustees of Princeton University

Inventor： Robert H. Austin ,  Zhaoning Yu ,  Jonas O. Tegenfeldt ,  Stephen Y. Chou ,  Han Cao

IPC: G01N27/447

Abstract: Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.

公开(公告)号：US20170343509A1

公开(公告)日：2017-11-30

申请号：US15621271

申请日：2017-06-13

Applicant: The Trustees of Princeton University

Inventor： Robert H. Austin ,  Zhaoning Yu ,  Jonas O. Tagenfeldt ,  Stephen Y. Chou ,  Han Cao

IPC: G01N27/447

CPC classification number: G01N27/44791 ,  G01N27/447

Abstract: Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.

公开(公告)号：US09678038B2

公开(公告)日：2017-06-13

申请号：US14081322

申请日：2013-11-15

Applicant: The Trustees of Princeton University

Inventor： Robert H. Austin ,  Zhaoning Yu ,  Jonas O. Tegenfeldt ,  Stephen Y. Chou ,  Han Cao

IPC: G01N27/447

CPC classification number: G01N27/44791 ,  G01N27/447

Abstract: Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.

公开(公告)号：US20140206555A1

公开(公告)日：2014-07-24

申请号：US14223589

申请日：2014-03-24

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Robert H. Austin ,  Zhaoning Yu ,  Jonas O. Tegenfeldt ,  Stephen Y. Chou ,  Han Cao

IPC: G01N33/487

CPC classification number: C12Q1/6869 ,  B01J2219/00274 ,  B01L3/502707 ,  B01L2200/0663 ,  B01L2200/12 ,  B01L2300/0627 ,  B01L2300/0861 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/086 ,  B81B1/002 ,  B81B2201/058 ,  B81B2203/0338 ,  B81C1/00071 ,  B82Y30/00 ,  C12Q1/6837 ,  G01N21/6428 ,  G01N21/648 ,  G01N33/48721 ,  Y10S977/704 ,  Y10S977/88 ,  Y10S977/883

Abstract: Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.

Abstract translation: 公开了能够实现高通量大分子分析的纳米通道阵列。 还公开了制备纳米通道阵列和纳米流体芯片的方法。 还公开了分析大分子的方法，例如基因组DNA的全部链，以及用于实施这些方法的系统。

公开(公告)号：US10161001B2

公开(公告)日：2018-12-25

申请号：US15178248

申请日：2016-06-09

Applicant: The Trustees of Princeton University

Inventor： Robert H. Austin ,  Zhaoning Yu ,  Jonas O. Tegenfeldt ,  Stephen Y. Chou ,  Han Cao

IPC: C12Q1/6869 ,  B01L3/00 ,  B81B1/00 ,  B81C1/00 ,  B82Y30/00 ,  G01N21/64 ,  G01N33/487 ,  C12Q1/6837

Abstract: Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.

公开(公告)号：US20140030811A1

公开(公告)日：2014-01-30

申请号：US13675685

申请日：2012-11-13

Applicant: Princeton University

Inventor： Han Cao ,  Jonas O. Tegenfeldt ,  Stephen Chou ,  Robert H. Austin

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0663 ,  B01L2200/12 ,  B01L2300/0654 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/086 ,  B81B2201/058 ,  B81C1/00119 ,  B81C2201/0157 ,  B81C2201/0159 ,  B82Y30/00 ,  G01N33/48721 ,  G03F7/2008 ,  Y10T436/143333

Abstract: The present invention relates to a device for interfacing nanofluidic and microfluidic components suitable for use in performing high throughput macromolecular analysis. Diffraction gradient lithography (DGL) is used to form a gradient interface between a microfluidic area and a nanofluidic area. The gradient interface area reduces the local entropic barrier to nanochannels formed in the nanofluidic area. In one embodiment, the gradient interface area is formed of lateral spatial gradient structures for narrowing the cross section of a value from the micron to the nanometer length scale. In another embodiment, the gradient interface area is formed of a vertical sloped gradient structure. Additionally, the gradient structure can provide both a lateral and vertical gradient.

Abstract translation: 本发明涉及适用于进行高通量大分子分析的纳米流体和微流体组分的界面装置。 衍射梯度光刻（DGL）用于在微流体区域和纳流体区域之间形成梯度界面。 梯度界面面积减小了在纳米流体区域形成的纳米通道的局部熵阻挡。 在一个实施例中，梯度界面区域由横向空间梯度结构形成，用于使从微米到纳米长度尺度的值的横截面变窄。 在另一个实施例中，梯度界面区域由垂直倾斜梯度结构形成。 此外，梯度结构可以提供横向和垂直梯度。

公开(公告)号：US20230110246A1

公开(公告)日：2023-04-13

申请号：US18059476

申请日：2022-11-29

Applicant: Princeton University

Inventor： Han Cao ,  Jonas O. Tegenfeldt ,  Stephen Chou ,  Robert H. Austin

IPC: G01N21/64 ,  B01L3/00 ,  B81C1/00 ,  B82Y30/00 ,  G01N33/487 ,  G03F7/20

Abstract: A fluidic chip includes at least one nanochannel array, the nanochannel array including a surface having a nanofluidic area formed in the material of the surface; a microfluidic area on said surface; a gradient interface area having a gradual elevation of height linking the microfluidic area and the nanofluidic area; and a sample reservoir capable of receiving a fluid in fluid communication with the microfluidic area. In another embodiment, a fluidic chip includes at least one nanochannel array, the nanochannel array includes a surface having a nanofluidic area formed in the material of the surface; a microfluidic area on said surface; and a gradient interface area linking the microfluidic area and the nanofluidic area, where the gradient interface area comprises a plurality of gradient structures, and the lateral spacing distance between said gradient structures decreases towards said nanofluidic area; and a sample reservoir capable of receiving a fluid in fluid communication with the microfluidic area.

公开(公告)号：US10768142B2

公开(公告)日：2020-09-08

申请号：US16367701

申请日：2019-03-28

Applicant: The Trustees of Princeton University

Inventor： Robert H. Austin ,  Zhaoning Yu ,  Jonas O. Tegenfeldt ,  Stephen Y. Chou ,  Han Cao

IPC: G01N27/447 ,  B01L3/00 ,  B81B1/00 ,  B81C1/00 ,  B82Y30/00 ,  C12Q1/6869 ,  G01N21/64 ,  G01N33/487 ,  C12Q1/6837

Abstract: Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.

公开(公告)号：US20200158644A1

公开(公告)日：2020-05-21

申请号：US16720205

申请日：2019-12-19

Applicant: Princeton University

Inventor： Han Cao ,  Jonas O. Tegenfeldt ,  Stephen Chou ,  Robert H. Austin

IPC: G01N21/64 ,  B01L3/00 ,  B81C1/00 ,  B82Y30/00 ,  G01N33/487 ,  G03F7/20

Abstract: A fluidic chip includes at least one nanochannel array, the nanochannel array including a surface having a nanofluidic area formed in the material of the surface; a microfluidic area on said surface; a gradient interface area having a gradual elevation of height linking the microfluidic area and the nanofluidic area; and a sample reservoir capable of receiving a fluid in fluid communication with the microfluidic area. In another embodiment, a fluidic chip includes at least one nanochannel array, the nanochannel array includes a surface having a nanofluidic area formed in the material of the surface; a microfluidic area on said surface; and a gradient interface area linking the microfluidic area and the nanofluidic area, where the gradient interface area comprises a plurality of gradient structures, and the lateral spacing distance between said gradient structures decreases towards said nanofluidic area; and a sample reservoir capable of receiving a fluid in fluid communication with the microfluidic area.