公开(公告)号：US20040127357A1

公开(公告)日：2004-07-01

申请号：US10689176

申请日：2003-10-20

Applicant: Promega Corporation

Inventor： Daniel J. Simpson ,  Tonny Johnson ,  Roderick G. Flemming

IPC: B01J020/22

CPC classification number: B01D15/3828 ,  B01J20/103 ,  B01J20/28009 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3234 ,  B01J20/3242 ,  B01J20/3251 ,  B01J20/3259 ,  B01J20/3265 ,  B01J20/3289 ,  B01J20/3293 ,  B01J45/00 ,  B01J2220/58 ,  B01J2220/66 ,  C02F1/285 ,  C02F2101/20 ,  C07K1/20 ,  G01N2030/042

Abstract: The present invention provides compositions for the separation of metals or biomolecules such as polypeptides, nucleic acids, or endotoxins using modified solid supports.

Abstract translation: 本发明提供了使用改性固体支持物分离金属或生物分子例如多肽，核酸或内毒素的组合物。

公开(公告)号：US20040185545A1

公开(公告)日：2004-09-23

申请号：US10689368

申请日：2003-10-20

Applicant: Promega Corporation

Inventor： Daniel J. Simpson ,  Tonny Johnson ,  John Shultz ,  Roderick G. Flemming ,  Rebecca Godat ,  Sanchayita Kar ,  Robin Hurst

IPC: C12N009/00

CPC classification number: B01D15/3828 ,  B01J20/103 ,  B01J20/28009 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3234 ,  B01J20/3242 ,  B01J20/3251 ,  B01J20/3259 ,  B01J20/3265 ,  B01J20/3289 ,  B01J20/3293 ,  B01J45/00 ,  B01J2220/58 ,  B01J2220/66 ,  C02F1/285 ,  C02F2101/20 ,  C07K1/20 ,  G01N2030/042

Abstract: The present invention provides compositions and methods for the separation of metals or molecules such as polypeptides, nucleic acids, or endotoxins using a metal-modified solid support. The metals or molecules are isolated from a starting material using the modified solid supports of the invention. Also provided by the invention are kits that can be used in connection with the inventive methods.

Abstract translation: 本发明提供使用金属改性固体支持物分离金属或分子例如多肽，核酸或内毒素的组合物和方法。 使用本发明的改性固体载体，从原料中分离金属或分子。 本发明还提供了可以结合本发明方法使用的试剂盒。

公开(公告)号：US20020001812A1

公开(公告)日：2002-01-03

申请号：US09912045

申请日：2001-07-24

Applicant: Promega Corporation.

Inventor： Craig E. Smith ,  Diana L. Holmes ,  Daniel J. Simpson ,  Jehoshua Katzhendler ,  Rex M. Bitner ,  Josephine C. Grosch

IPC: C12Q001/68 ,  G01N031/00 ,  C07H021/02 ,  A01N057/00 ,  C07H021/04 ,  C12P019/34 ,  A61K031/665

CPC classification number: C12N15/1006 ,  B01J41/20 ,  B01J47/04 ,  C12N15/101 ,  C12N15/1013 ,  Y10S435/803 ,  Y10T436/107497

Abstract: Mixed-bed solid phases are provided, with methods for using such solid phases to isolate target nucleic acids, such as plasmid DNA, chromosomal DNA, RNA, or nucleic acids generated by enzymatic amplification from contaminants, including proteins, lipids, cellular debris, or other nucleic acids. The mixed-bed solid phases of this invention are mixtures of at least two different solid phases, each of which has a capacity to bind to the target nucleic acid under different solution conditions, and the capacity to release the nucleic acid under similar elution conditions. By exchanging solution conditions according to the methods of this invention, one can remove contaminants from the target nucleic acid bound to the mixed-bed solid phase, then elute the target nucleic acid in an elution buffer.

公开(公告)号：US20010014650A1

公开(公告)日：2001-08-16

申请号：US09813077

申请日：2001-03-20

Applicant: Promega Corporation

Inventor： Graig E. Smith ,  Diana L. Holmes ,  Daniel J. Simpson ,  Jehoshua Katzenhendler ,  Rex M. Bitner ,  Josephine C. Grosch

IPC: B01J020/22 ,  B01J020/10

CPC classification number: C12N15/101 ,  B01J39/26 ,  B01J41/20

Abstract: pH dependent ion exchange matrices are provided, with methods for making such matrices, and methods for using such matrices to isolate a target nucleic acid, such as plasmid DNA, chromosomal DNA, or RNA from contaminants, including proteins, lipids, cellular debris, or other nucleic acids. Each pH dependent ion exchange matrix of this invention comprises at least two different ion exchange functional groups, one of which is capable of acting as an anion exchanger at a first pH, and the other of which is capable of acting as a cation exchanger at a second, higher pH. The matrix has an overall neutral charge in a pH range between the first and second pH. The pH dependent ion exchange matrices of the present invention are designed to bind to the target nucleic acid at a pH wherein the overall charge of the matrix is positive, and to release the target nucleic acid as the pH of the surrounding solution is increased. The target nucleic acid can be released from the pH dependent matrix in little or no salt and at about a neutral pH. The matrices and methods of this invention enable one to isolate a target nucleic acid in very few steps, without the use of hazardous chemicals. Target nucleic acids isolated using the pH dependent ion exchange matrices according to the present invention can be used immediately without further extraction or isolation.

Abstract translation: 提供了依赖于pH的离子交换基质，以及用于制备这种基质的方法，以及使用这些基质从污染物（包括蛋白质，脂质，细胞碎片）分离目标核酸（例如质粒DNA，染色体DNA或RNA）的方法 其他核酸。 本发明的每个pH依赖性离子交换基质包含至少两种不同的离子交换官能团，其中之一能够在第一pH下作为阴离子交换剂，另一种能够在 第二，较高的pH值。 该基质在第一和第二pH之间的pH范围内具有总体中性电荷。 本发明的pH依赖性离子交换基质被设计成在其中基质的总电荷为阳性的pH下与靶核酸结合，并且随着周围溶液的pH增加而释放靶核酸。 目标核酸可以很少或没有盐和约中性pH从pH依赖性基质释放。 本发明的基质和方法使得能够以非常少的步骤分离靶核酸，而不使用危险化学品。 使用根据本发明的pH依赖性离子交换基质分离的靶核酸可以立即使用，无需进一步提取或分离。