公开(公告)号：US20230416724A1

公开(公告)日：2023-12-28

申请号：US18212882

申请日：2023-06-22

Applicant: Purigen Biosystems, Inc.

Inventor： Nathan P. Hoverter ,  Lewis A. Marshall ,  Klint A. Rose ,  Debannita Datta ,  Douglas Fadrosh

IPC: C12N15/10 ,  G01N27/447

CPC classification number: C12N15/101 ,  G01N27/44721

Abstract: The present disclosure provides cell lysis buffers and method of use. Cell lysis buffers may comprise an aqueous solution comprising a buffering agent, a detergent, and cyanate or thiocyanate. The cell lysis buffers may be free or substantially free of urea and/or carbonic acid. Such lysis buffers may be used to lyse cells, e.g., in tissues. The cell lysis buffers may be suitable for use with isotachophoresis.