公开(公告)号：US12065466B2

公开(公告)日：2024-08-20

申请号：US17326247

申请日：2021-05-20

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Manjula Pandey

IPC: C07K14/00 ,  G01N33/68

CPC classification number: C07K14/00 ,  C07K14/003 ,  C07K2319/00 ,  C07K2319/70 ,  G01N33/6818

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides amino acid recognition molecules, such as amino acid binding proteins and fusion polypeptides thereof. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

公开(公告)号：US20210364527A1

公开(公告)日：2021-11-25

申请号：US17326247

申请日：2021-05-20

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Manjula Pandey

IPC: G01N33/68 ,  C07K14/00

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides amino acid recognition molecules, such as amino acid binding proteins and fusion polypeptides thereof. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

公开(公告)号：US20210102180A1

公开(公告)日：2021-04-08

申请号：US16914195

申请日：2020-06-26

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Manjula Pandey

IPC: C12N9/12 ,  C12N11/00 ,  C12Q1/6806 ,  C12P19/34

Abstract: Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.

公开(公告)号：US20180208911A1

公开(公告)日：2018-07-26

申请号：US15846967

申请日：2017-12-19

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Mohammad Wadud Bhuiya ,  Manjula Pandey ,  Jeremy Lackey ,  Jonathan M. Rothberg

IPC: C12N9/12 ,  C12Q1/6869

CPC classification number: C12N9/1252 ,  C07K2319/00 ,  C12P19/34 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.

公开(公告)号：US20230213527A1

公开(公告)日：2023-07-06

申请号：US18145815

申请日：2022-12-22

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Manjula Pandey ,  David Kamber ,  Kenneth Skinner

IPC: G01N33/68

CPC classification number: G01N33/6818 ,  G01N33/6893 ,  G01N2440/12 ,  G01N2440/18 ,  G01N2440/10 ,  G01N2440/14 ,  G01N2800/102 ,  G01N2800/2821 ,  G01N2800/32

Abstract: Aspects of the application relate to methods and systems for obtaining information regarding multiple amino acids in a polypeptide based on binding interactions between the polypeptide and one or more amino acid recognizers. Kinetic signature information may be obtained from a series of signal pulses indicative of a series of binding events between one or more amino acid recognizers and an amino acid of a polypeptide (e.g., a terminal amino acid, an internal amino acid). The kinetic signature information (e.g., pulse duration, interpulse duration, recognition segment (RS) duration, intersegment duration) may be used to determine one or more chemical characteristics (e.g., identity, modification) of multiple amino acids of the polypeptide.

公开(公告)号：US20230058852A1

公开(公告)日：2023-02-23

申请号：US17690920

申请日：2022-03-09

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Mohammad Wadud Bhuiya ,  Manjula Pandey ,  Jeremy Lackey ,  Jonathan M. Rothberg ,  Thomas Christian

IPC: C12N9/12 ,  C12Q1/6869 ,  C12P19/34 ,  C12N15/52

Abstract: Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.

公开(公告)号：US20180362941A9

公开(公告)日：2018-12-20

申请号：US15846967

申请日：2017-12-19

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Mohammad Wadud Bhuiya ,  Manjula Pandey ,  Jeremy Lackey ,  Jonathan M. Rothberg

IPC: C12N9/12 ,  C12Q1/6869

Abstract: Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.

公开(公告)号：US20250002877A1

公开(公告)日：2025-01-02

申请号：US18603163

申请日：2024-03-12

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Manjula Pandey

IPC: C12N9/12 ,  C12N11/00 ,  C12P19/34 ,  C12Q1/6806

Abstract: Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.

公开(公告)号：US20240272170A1

公开(公告)日：2024-08-15

申请号：US18365185

申请日：2023-08-03

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Manjula Pandey ,  Marco Ribezzi-Crivellari ,  Douglas Pike ,  Jennifer Tullman ,  Kristin Blacklock ,  Adeline Pichard-Kostuch ,  Ahmed Rehan ,  Mohammad Wadud Bhuiya

IPC: G01N33/68 ,  C07K14/37

CPC classification number: G01N33/6824 ,  C07K14/37

Abstract: There is provided amino acid recognizers with improved binding properties, allowing for more structural information to be obtained from polypeptides based on the kinetics of on-off binding between recognizer and polypeptide. An amino acid recognizer may comprise an amino acid binding protein with an engineered binding pocket having one or more modifications relative to a homologous protein. The modified binding pocket may increase the number of interactions formed between the binding pocket and an amino acid ligand as compared to an unmodified binding pocket of the homologous protein, increase the number of types of amino acid ligands capable of being detectably bound as compared to an unmodified binding pocket of the homologous protein, and improve the kinetics of binding (e.g., KD, koff, kon) toward one or more types of amino acid ligands, which advantageously increases the amount of, or confidence in, structural information that may be derived from polypeptide analysis.

公开(公告)号：US20240228671A1

公开(公告)日：2024-07-11

申请号：US18491727

申请日：2023-10-20

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Haidong Huang ,  Manjula Pandey ,  Andrzej Wilczynski

IPC: C07K19/00 ,  C07K7/04 ,  C12Q1/37 ,  G01N33/68

CPC classification number: C07K19/00 ,  C07K7/04 ,  C12Q1/37 ,  G01N33/6818

Abstract: Provided herein are compounds of Formulae (I) and (II), which comprise polypeptidyl groups. Also provided herein are methods of preparing compounds of Formulae (I) and (II). Further provided herein are methods of sequencing a polypeptide by reaction of compounds of Formula (II) with peptidases.