公开(公告)号：US12065466B2

公开(公告)日：2024-08-20

申请号：US17326247

申请日：2021-05-20

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Manjula Pandey

IPC: C07K14/00 ,  G01N33/68

CPC classification number: C07K14/00 ,  C07K14/003 ,  C07K2319/00 ,  C07K2319/70 ,  G01N33/6818

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides amino acid recognition molecules, such as amino acid binding proteins and fusion polypeptides thereof. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

公开(公告)号：US20240272169A1

公开(公告)日：2024-08-15

申请号：US18598736

申请日：2024-03-07

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Jeremy Lackey ,  Haidong Huang

IPC: G01N33/58 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/64 ,  G01N33/68 ,  G16B25/10 ,  G16B30/00 ,  G16B40/00 ,  G16B40/10 ,  G16B50/00 ,  G16B50/30

CPC classification number: G01N33/581 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/6428 ,  G01N33/58 ,  G01N33/582 ,  G01N33/6821 ,  G01N33/6824 ,  G16B25/10 ,  G16B40/00 ,  G16B40/10 ,  G16B50/30 ,  G01N2021/6439 ,  G01N2458/00 ,  G16B30/00 ,  G16B50/00

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

公开(公告)号：US12055548B2

公开(公告)日：2024-08-06

申请号：US16709077

申请日：2019-12-10

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Jeremy Lackey ,  Haidong Huang

IPC: A61K38/00 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/64 ,  G01N33/58 ,  G01N33/68 ,  G16B25/10 ,  G16B40/00 ,  G16B40/10 ,  G16B50/30 ,  G16B30/00 ,  G16B50/00

CPC classification number: G01N33/581 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/6428 ,  G01N33/58 ,  G01N33/582 ,  G01N33/6821 ,  G01N33/6824 ,  G16B25/10 ,  G16B40/00 ,  G16B40/10 ,  G16B50/30 ,  G01N2021/6439 ,  G01N2458/00 ,  G16B30/00 ,  G16B50/00

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

公开(公告)号：US11719639B2

公开(公告)日：2023-08-08

申请号：US17190331

申请日：2021-03-02

Applicant: Quantum-Si Incorporated

Inventor： Gerard Schmid ,  Dajiang Yang ,  Eric A. G. Webster ,  Xin Wang ,  Todd Rearick ,  Changhoon Choi ,  Ali Kabiri ,  Kyle Preston ,  Brian Reed

IPC: G01N21/64 ,  H01L27/146

CPC classification number: G01N21/6454 ,  G01N21/6408 ,  G01N21/6428 ,  H01L27/14603 ,  G01N2021/6463

Abstract: Some aspects relate to an integrated circuit, comprising at least one photodetection region configured to generate charge carriers responsive to incident photons emitted from a sample, at least one charge storage region configured to receive the charge carriers from the photodetection region, and at least one controller configured to obtain information about the incident photons, the information comprising at least one member selected from the group comprising pulse duration and interpulse duration and at least one member selected from the group comprising wavelength information, luminescence lifetime information, and intensity information. In some embodiments, the information comprises at least three, four, and/or five members selected from the group comprising wavelength information, luminescence lifetime information, intensity information, pulse duration information, and interpulse duration information. In some embodiments, the information obtained may be used to identify the sample.

公开(公告)号：US20230221253A1

公开(公告)日：2023-07-13

申请号：US18095767

申请日：2023-01-11

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Todd Rearick ,  Gerard Schmid ,  Haidong Huang

IPC: G01N21/64 ,  G01N33/68 ,  G01N33/58 ,  G01N1/28

CPC classification number: G01N21/6428 ,  G01N33/6821 ,  G01N33/6824 ,  G01N33/582 ,  G01N1/28 ,  G01N2021/6439 ,  G01N2458/00 ,  G16B30/00

Abstract: Techniques for multi-dimensional signal analysis are described herein. The techniques may be used in one or more sequencing applications. For example, according to some aspects, there is provided a method comprising: determining information about a sample that emits emission light in response to excitation light based on at least one of pulse duration and interpulse duration and at least two of wavelength, intensity, and lifetime of the emission light, wherein the sample comprises a reagent configured to be coupled to a luminescent label, and wherein a shielding element is disposed between the reagent and the luminescent label.

公开(公告)号：US20220073973A1

公开(公告)日：2022-03-10

申请号：US17314626

申请日：2021-05-07

Applicant: Quantum-Si incorporated

Inventor： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang

IPC: C12Q1/6818 ,  C12Q1/6869 ,  G01N21/64 ,  C12Q1/6874

Abstract: Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.

公开(公告)号：US20210364527A1

公开(公告)日：2021-11-25

申请号：US17326247

申请日：2021-05-20

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Manjula Pandey

IPC: G01N33/68 ,  C07K14/00

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides amino acid recognition molecules, such as amino acid binding proteins and fusion polypeptides thereof. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

公开(公告)号：US20200219590A1

公开(公告)日：2020-07-09

申请号：US16686028

申请日：2019-11-15

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Jeremy Lackey ,  Thomas Christian ,  Roger Nani ,  David Dodd ,  Kathren Croce ,  Alexander Goryaynov ,  Jonathan M. Rothberg

IPC: G16B50/30 ,  G16B25/10 ,  G16B40/00 ,  C12Q1/6806

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

公开(公告)号：US20200209253A1

公开(公告)日：2020-07-02

申请号：US16708956

申请日：2019-12-10

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Jeremy Lackey ,  Thomas Christian ,  Roger Nani ,  David Dodd ,  Kathren Croce ,  Alexander Goryaynov ,  Jonathan M. Rothberg

IPC: G01N33/68 ,  G01N33/58 ,  G16B30/00

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

公开(公告)号：US20180223353A1

公开(公告)日：2018-08-09

申请号：US15847001

申请日：2017-12-19

Applicant: Quantum-Si Incorporated

Inventor： James A. Ball ,  Jeremy Lackey ,  Brian Reed ,  Alexander Goryaynov ,  Jonathan M. Rothberg

IPC: C12Q1/6869 ,  B01L9/00 ,  B01L3/00 ,  C08L1/02 ,  C08L71/08

Abstract: Methods of loading a molecule of interest into a sample well are provided. In some aspects, methods of loading a molecule of interest into a sample well involve loading a molecule of interest into a sample well in the presence of a crowding agent and/or a condensing agent. In some aspects, methods of loading a sequencing template into a sample well are provided.