公开(公告)号：US20210009660A1

公开(公告)日：2021-01-14

申请号：US15934055

申请日：2018-03-23

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Alfred Engel ,  Pavel Kubalec ,  Alfons Nichtl ,  Tobias Oelschlaegel ,  Rainer Schlecht

IPC: C07K16/00

Abstract: The disclosure describes a recombinant immunoglobulin heavy chain comprising a sortase conjugation loop, methods for conjugating and/or labeling such recombinant immunoglobulin heavy chains by use of the enzyme sortase and to the conjugates/labeled products obtained via the method.

公开(公告)号：US08981070B2

公开(公告)日：2015-03-17

申请号：US13866412

申请日：2013-04-19

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Simone Budow ,  Ping Ding ,  Dieter Heindl ,  Alfons Nichtl ,  Frank Seela

IPC: C07H21/02 ,  C07H21/00 ,  C12Q1/68

CPC classification number: C12Q1/6876 ,  C07H21/02

Abstract: An oligonucleotide-solid phase conjugate, wherein the solid phase is thiophilic and the oligonucleotide comprises at least one thiooxonucleobase according to Formula I, wherein X is CH or N, R1 is H or NH2, --- indicates a covalent bond, and said oligonucleotide is bound to said solid phase via the sulfur atom of said thiooxonucleotide. Also disclosed are methods for producing such conjugate as well as various uses for such oligonucleotide metal conjugate.

Abstract translation: 一种寡核苷酸 - 固相缀合物，其中所述固相是亲核的，并且所述寡核苷酸包含至少一种根据式I的硫氧基核碱基，其中X为CH或N，R 1为H或NH 2，表示共价键，所述寡核苷酸 通过所述硫代核苷酸的硫原子与所述固相结合。 还公开了用于生产这种缀合物的方法以及这种寡核苷酸金属缀合物的各种用途。

公开(公告)号：US11136376B2

公开(公告)日：2021-10-05

申请号：US15934055

申请日：2018-03-23

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Alfred Engel ,  Pavel Kubalec ,  Alfons Nichtl ,  Tobias Oelschlaegel ,  Rainer Schlecht

IPC: C07K16/00

Abstract: The disclosure describes a recombinant immunoglobulin heavy chain comprising a sortase conjugation loop, methods for conjugating and/or labeling such recombinant immunoglobulin heavy chains by use of the enzyme sortase and to the conjugates/labeled products obtained via the method.

公开(公告)号：US20180172680A1

公开(公告)日：2018-06-21

申请号：US15899003

申请日：2018-02-19

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Toralf Zarnt ,  Dieter Heindl ,  Alfons Nichtl ,  Frank Sicherl-Birk

IPC: G01N33/543

Abstract: Disclosed is a method for measurement of an analyte in a microparticle-based analyte-specific binding assay, wherein the microparticles are coated with the first partner of a binding pair, the method involving mixing the coated microparticles, an analyte-specific binding agent conjugated to the second partner of the binding pair, and a sample suspected of containing or containing the analyte, wherein the second partner of the binding pair is bound to the analyte-specific binding agent via a linker having from 12 to 30 ethylene glycol units (PEG 12 to 30), thereby binding the analyte via the conjugated analyte-specific binding agent to the coated microparticles, separating the microparticles having the analyte bound via the binding pair and the analyte-specific binding agent from the mixture and measuring the analyte bound to the microparticles.