公开(公告)号：US20210180095A1

公开(公告)日：2021-06-17

申请号：US16762336

申请日：2018-11-09

Applicant: SHELL OIL COMPANY

Inventor： Karl Alex Peter PAYNE ,  David LEYS ,  David Alexander PARKER ,  Stephen Andrew MARSHALL

IPC: C12P17/04 ,  C12N9/88

Abstract: Method for preparing a 2,5-furandicarboxylic acid (“FDCA”) comprising: contacting a polypeptide comprising an amino acid sequence that has greater than 34% sequence identity with an amino acid sequence set out in SEQ ID NO:1 or SEQ ID NO:2 with furoic acid in the presence of carbon dioxide; wherein the polypeptide has carboxylase and decarboxylase activity and comprises (i) the amino acid corresponding to H297 or a functional substitution thereof and (ii) at least one of (a) the amino acid corresponding to R305 or a functional substitution thereof; and (b) the amino acid corresponding to R332 or a functional substitution thereof; wherein the position is numbered relative to the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:2.

公开(公告)号：US20130330795A1

公开(公告)日：2013-12-12

申请号：US13915059

申请日：2013-06-11

Applicant: SHELL OIL COMPANY

Inventor： David LEYS ,  Karl Alex Peter Payne ,  Nigel Shaun Scrutton ,  David Alexander Parker ,  Andrew James Murphy

IPC: C12P5/02 ,  C12P5/00

CPC classification number: C12P5/026 ,  C10G3/42 ,  C10G3/50 ,  C10G45/58 ,  C10L1/02 ,  C10L1/04 ,  C12N9/88 ,  C12P5/005 ,  C12Y401/01 ,  Y02P30/20

Abstract: Method for preparing a mono-unsaturated alkene comprising contacting an aliphatic mono-unsaturated carboxylic acid with an Fdc1 polypeptide comprising an amino acid sequence with at least 21% sequence identity to SEQ ID NO: 1 and a Pad1 polypeptide comprising an amino acid sequence with at least 17% sequence identity to SEQ ID NO: 2.

Abstract translation: 制备单不饱和烯烃的方法，包括使脂族单不饱和羧酸与包含与SEQ ID NO：1具有至少21％序列同一性的氨基酸序列的Fdc1多肽和包含氨基酸序列的Pad1多肽接触， 与SEQ ID NO：2具有至少17％的序列同一性。