Abstract:
A fuel composition comprising: a diesel base fuel; from 1 to 10% v/v of a fatty acid alkyl ester; and more than 10% v/v of an ether component, the ether component comprising one or more ether compounds having in the range of from 8 to 12 carbon atoms and selected from compounds of formula I R1—O—R2 (I) wherein R1 and R2 are independently primary or secondary alkyl.
Abstract:
Method for converting a carbon source such as a plant-derived material into a hydrocarbon or hydrocarbon precursor, via a microorganism-catalysed aerobic reaction. The microorganism is cultivated in the presence of the carbon source and of oxygen, in a reaction vessel having a capacity of about 2000 litres or greater which comprises an aeration system for supplying oxygen to the microorganism. Also provided is an apparatus for use in the method, comprising a reaction vessel of capacity 2000 litres or greater, and an aeration system.
Abstract:
Method for preparing a 2,5-furandicarboxylic acid (“FDCA”) comprising: contacting a polypeptide comprising an amino acid sequence that has greater than 34% sequence identity with an amino acid sequence set out in SEQ ID NO:1 or SEQ ID NO:2 with furoic acid in the presence of carbon dioxide; wherein the polypeptide has carboxylase and decarboxylase activity and comprises (i) the amino acid corresponding to H297 or a functional substitution thereof and (ii) at least one of (a) the amino acid corresponding to R305 or a functional substitution thereof; and (b) the amino acid corresponding to R332 or a functional substitution thereof; wherein the position is numbered relative to the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:2.
Abstract:
Sacchromyces cerevisiae strains modified to express an adhE (alcohol/aldehyde dehydrogenase) enzyme having the amino acid sequence SEQ ID NO:1, or a functional variant thereof capable of catalysing the conversion of acetyl CoA to ethanol, and/or to overexpress an ACS2 (acetyl-CoA synthetase) enzyme having the amino acid sequence SEQ ID NO:2, or a functional variant thereof capable of catalysing the conversion of acetate to acetyl CoA. Such cells have an improved ability to grow on or in acetate-containing media compared to cells not according to the invention.
Abstract translation:酿酒酵母菌株被修饰以表达具有氨基酸序列SEQ ID NO:1的adhE(醇/醛脱氢酶)或其能够催化乙酰CoA转化为乙醇的功能变体,和/或过表达ACS2( 具有氨基酸序列SEQ ID NO:2的乙酰辅酶A合成酶)或其能够催化乙酸转化为乙酰辅酶A的功能变体。 与不是本发明的细胞相比,这样的细胞具有改善的在含乙酸酯培养基上或含乙酸酯培养基中生长的能力。