公开(公告)号：US11169074B2

公开(公告)日：2021-11-09

申请号：US16606518

申请日：2018-02-20

Applicant: SONY CORPORATION

Inventor： Gakuji Hashimoto ,  Tatsumi Ito ,  Kazuya Takahashi ,  Junji Kajihara

IPC: G01N15/14 ,  B01L3/00

Abstract: In the present technology, the timing at which suction is performed is optimized in order to enhance the microparticle separation performance in a technology for separating target microparticles in a microchip. For this purpose, the present technology provides a method of optimizing a microparticle suction condition, and the like, using a microchip having a main flow channel through which a liquid containing a microparticle flows, a microparticle suction flow channel arranged coaxially with the main flow channel, and a branch flow channel branching from the main flow channel. The method includes: a branch point specifying process of specifying a branch point at which the branch flow channel branches from the main flow channel; and a time assignment process of assigning a time T1 to be applied to suction of the microparticle.

公开(公告)号：US20140113287A1

公开(公告)日：2014-04-24

申请号：US14047716

申请日：2013-10-07

Applicant: Sony Corporation

Inventor： Tomohiko Nakamura ,  Junji Kajihara

IPC: C12Q1/68

CPC classification number: G01N21/6486 ,  B01L7/52 ,  C12Q1/68 ,  C12Q1/6816 ,  G01N21/0332 ,  G01N21/6408 ,  G01N2201/06113 ,  G01N2201/062 ,  C12Q2527/107

Abstract: There is provided a base sequence analysis method including a nucleic acid amplification procedure of obtaining an amplification product by a nucleic acid amplification reaction, a turbidity measurement procedure of measuring turbidity of a reaction solution of the nucleic acid amplification reaction, and a melting curve analysis procedure of performing melting curve analysis of a probe nucleic acid chain and the amplification product at a reaction site of the nucleic acid amplification reaction.

Abstract translation: 提供了碱基序列分析方法，其包括通过核酸扩增反应获得扩增产物的核酸扩增方法，测量核酸扩增反应的反应溶液的浊度的浊度测定程序和熔解曲线分析步骤 在核酸扩增反应的反应位点进行探针核酸链和扩增产物的融合曲线分析。

公开(公告)号：US20210018424A1

公开(公告)日：2021-01-21

申请号：US17042858

申请日：2019-04-02

Applicant: Sony Corporation

Inventor： Junji Kajihara ,  Yoshiki Okamoto

IPC: G01N15/14 ,  B01L3/00 ,  B03C5/02

Abstract: There is provided a microchip including a plurality of substrate layers having a flow path in which a liquid containing microparticles flows in at least one of the substrate layers, the microchip at least including: an optical radiation region in which light is radiated to microparticles contained in a fluid flowing in the flow path from a side surface of the substrate layers.

公开(公告)号：US20200072732A1

公开(公告)日：2020-03-05

申请号：US16614020

申请日：2018-02-14

Applicant: Sony Corporation

Inventor： Gakuji Hashimoto ,  Tatsumi Ito ,  Kazuya Takahashi ,  Junji Kajihara

IPC: G01N15/14 ,  B01L3/00

Abstract: To provide a technology of optimizing a suction condition of a microparticle.The present technology providesan optimizing method of a suction condition of a microparticle including:a particle number counting step of detecting a time point when a microparticle passes through a predetermined position on a main flow path through which liquid containing the microparticle flows, sucking the microparticle from the main flow path to a microparticle suction flow path by the microparticle suction flow path with a predetermined suction force, and counting the number of microparticles sucked into the microparticle suction flow path; and a step of determining an elapsed time from passage through the predetermined position with which the suction by the microparticle suction flow path should be performedon the basis of a time from the time point when the microparticle passes through the predetermined position on the main flow path until the suction is performed and the number of counted microparticles.

公开(公告)号：US09102978B2

公开(公告)日：2015-08-11

申请号：US14047716

申请日：2013-10-07

Applicant: Sony Corporation

Inventor： Tomohiko Nakamura ,  Junji Kajihara

IPC: C12Q1/68

CPC classification number: G01N21/6486 ,  B01L7/52 ,  C12Q1/68 ,  C12Q1/6816 ,  G01N21/0332 ,  G01N21/6408 ,  G01N2201/06113 ,  G01N2201/062 ,  C12Q2527/107

Abstract: There is provided a base sequence analysis method including a nucleic acid amplification procedure of obtaining an amplification product by a nucleic acid amplification reaction, a turbidity measurement procedure of measuring turbidity of a reaction solution of the nucleic acid amplification reaction, and a melting curve analysis procedure of performing melting curve analysis of a probe nucleic acid chain and the amplification product at a reaction site of the nucleic acid amplification reaction.

Abstract translation: 提供了碱基序列分析方法，其包括通过核酸扩增反应获得扩增产物的核酸扩增方法，测量核酸扩增反应的反应溶液的浊度的浊度测定程序和熔解曲线分析步骤 在核酸扩增反应的反应位点进行探针核酸链和扩增产物的融合曲线分析。

公开(公告)号：US11525767B2

公开(公告)日：2022-12-13

申请号：US16614020

申请日：2018-02-14

Applicant: Sony Corporation

Inventor： Gakuji Hashimoto ,  Tatsumi Ito ,  Kazuya Takahashi ,  Junji Kajihara

IPC: G01N15/14 ,  B01L3/00

Abstract: To provide a technology of optimizing a suction condition of a microparticle.
The present technology provides an optimizing method of a suction condition of a microparticle including: a particle number counting step of detecting a time point when a microparticle passes through a predetermined position on a main flow path through which liquid containing the microparticle flows, sucking the microparticle from the main flow path to a microparticle suction flow path by the microparticle suction flow path with a predetermined suction force, and counting the number of microparticles sucked into the microparticle suction flow path; and a step of determining an elapsed time from passage through the predetermined position with which the suction by the microparticle suction flow path should be performed on the basis of a time from the time point when the microparticle passes through the predetermined position on the main flow path until the suction is performed and the number of counted microparticles.

公开(公告)号：US09976960B2

公开(公告)日：2018-05-22

申请号：US14793176

申请日：2015-07-07

Applicant: Sony Corporation

Inventor： Tomohiko Nakamura ,  Junji Kajihara

IPC: C12Q1/68 ,  G01N21/64 ,  B01L7/00 ,  G01N21/03

CPC classification number: G01N21/6486 ,  B01L7/52 ,  C12Q1/68 ,  C12Q1/6816 ,  G01N21/0332 ,  G01N21/6408 ,  G01N2201/06113 ,  G01N2201/062 ,  C12Q2527/107

Abstract: There is provided a base sequence analysis method including: a nucleic acid amplification procedure of obtaining an amplification product by a nucleic acid amplification reaction, a turbidity measurement procedure of measuring turbidity of a reaction solution of the nucleic acid amplification reaction; and a melting curve analysis procedure of performing melting curve analysis of a probe nucleic acid chain and the amplification product at a reaction site of the nucleic acid amplification reaction. This base sequence analysis method makes it possible to consecutively perform a nucleic acid amplification reaction and melting curve analysis at the same reaction site.

公开(公告)号：US20150139866A1

公开(公告)日：2015-05-21

申请号：US14401764

申请日：2013-03-13

Applicant: Sony Corporation

Inventor： Hidetoshi Watanabe ,  Yuji Segawa ,  Junji Kajihara ,  Kensuke Kojima ,  Toshio Watanabe ,  Masahiro Matsumoto

IPC: B01L3/00

CPC classification number: B01L3/5027 ,  B01L3/502707 ,  B01L2200/0642 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/123 ,  G01N2035/00158

Abstract: Provided is a microchip which enables to easily confirm, from the outside of the microchip, filling of a solution into an analysis region.The microchip includes: an introduction part for introducing a liquid; an analysis region in which a substance contained in the liquid or a reaction product of the substance is analyzed; an indication region to indicate that the analysis region has completely been filled with the liquid; and a flow channel to connect the introduction part, the analysis region, and the indication region. In the microchip, the flow channel is configured in such a manner that an amount of time it takes for the liquid introduced from the introduction part to reach the indication region is longer than an amount of time it takes for the liquid introduced from the introduction part to fill up the analysis region.

Abstract translation: 提供一种微芯片，其能够从微芯片的外部容易地确认将溶液填充到分析区域中。 微芯片包括：用于引入液体的引入部分; 其中分析液体中所含的物质或物质的反应产物的分析区域; 用于指示分析区域已经完全填充了液体的指示区域; 以及用于连接引入部分，分析区域和指示区域的流动通道。 在微芯片中，流路形成为从导入部导入的液体到达指示区域所花费的时间长于从导入部导入的液体所花费的时间 填补分析区域。

公开(公告)号：US11226291B2

公开(公告)日：2022-01-18

申请号：US16486642

申请日：2017-12-18

Applicant: SONY CORPORATION

Inventor： Junji Kajihara

IPC: G01N21/64 ,  G01N33/543 ,  G02B21/06 ,  G02B21/00

Abstract: The present technology provides an irradiation method, a biological substance analysis method, and a biological substance analyzer to improve a throughput while securing signal reliability of detection data when a biological substance is caught on a substrate and the biological substance is detected by irradiating the substrate. For this, the present technology provides a biological substance analysis method and the like including: a step of segmenting a substrate on which a molecule that can be bound to a biological substance is immobilized and the biological substance is bound to the molecule, and irradiating the substrate in accordance with an irradiation pattern having a different segment with time; and a step of analyzing the biological substance on the basis of information obtained from the irradiation.

公开(公告)号：US10956547B2

公开(公告)日：2021-03-23

申请号：US16151878

申请日：2018-10-04

Applicant: SONY CORPORATION

Inventor： Kenji Yamamoto ,  Hideo Sato ,  Isao Ichimura ,  Toshio Watanabe ,  Shinichi Kai ,  Junji Kajihara ,  Kengo Hayasaka

IPC: G06F21/32 ,  G06K9/00 ,  G06K9/20

Abstract: A biometrics authentication system having a small and simple configuration and being capable of implementing both of biometrics authentication and position detection is provided. A biometrics authentication system includes a light source emitting light to an object, a microlens array section condensing light from the object, a light-sensing device obtaining light detection data of the object on the basis of the light condensed by the microlens array section, a position detection section detecting the position of the object on the basis of the light detection data obtained in the light-sensing device, and an authentication section, in the case where the object is a living body, performing authentication of the living body on the basis of the light detection data obtained in the light-sensing device.