公开(公告)号：US20230314442A1

公开(公告)日：2023-10-05

申请号：US18012054

申请日：2021-06-30

Applicant: STRECK, INC.

Inventor： Matthew R. Sobansky ,  Nicholas Michael George ,  Bradford A. Hunsley ,  Sean Salamifar ,  Agnes Alicia Lenagh

IPC: G01N33/68

CPC classification number: G01N33/6842 ,  G01N2570/00 ,  G01N33/6848

Abstract: Provided herein are methods of preparing a protein sample for proteomic analysis. In exemplary embodiments, the method comprises (a) contacting a blood sample comprising proteins with a protective agent comprising an anticoagulant (AC) and an aldehyde releaser (AR), to obtain a mixture, optionally, wherein the blood sample is added to a blood collection tube (BCT) comprising the protective agent, and (b) isolating a fraction comprising proteins or a source of proteins from the mixture to yield a protein sample or a source of a protein sample, wherein steps of the method are carried out in the absence of exogenous proteolytic enzyme inhibitors, wherein the protein sample is suitable for proteomic and peptidomic analysis.

公开(公告)号：US20220074949A1

公开(公告)日：2022-03-10

申请号：US17417234

申请日：2019-12-27

Applicant: STRECK, INC.

Inventor： Matthew R. Sobansky

IPC: G01N33/68 ,  G01N1/40 ,  G01N30/72 ,  G01N30/88 ,  G01N30/86

Abstract: Provided herein are methods of preparing a protein sample for proteomic analysis. In exemplary embodiments, the method comprises (a) contacting a blood sample comprising proteins with a protective agent comprising an anticoagulant (AC) and an aldehyde releaser (AR), to obtain a mixture, optionally, wherein the blood sample is added to a blood collection tube (BCT) comprising the protective agent, and (b) isolating a fraction comprising proteins or a source of proteins from the mixture to yield a protein sample or a source of a protein sample, wherein steps of the method are carried out in the absence of exogenous proteolytic enzyme inhibitors, wherein the protein sample is suitable for proteomic analysis. Preferably, the protective agent consists of essentially (i) about 300 g/l to about 700 g/l imidazolidinyl urea; (ii) about 20 g/l to about 60 g/l glycine; and (iii) about 60 g/l to about 100 g/l EDTA; and the protein sample is analysed via mass spectrometry-based proteomic methods.