公开(公告)号：US20170362553A1

公开(公告)日：2017-12-21

申请号：US15695633

申请日：2017-09-05

Applicant: Sysmex Corporation

Inventor： Kanako MASUMOTO ,  Takuya KUBO ,  Shigeki IWANAGA ,  Masaya OKADA

IPC: C12M1/34 ,  G01N21/64 ,  G01N33/50 ,  G06T7/00

CPC classification number: C12M1/34 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/5005 ,  G01N2021/6432 ,  G01N2021/6441 ,  G06K9/00134 ,  G06K9/00147 ,  G06T7/0012 ,  G06T2207/30072

Abstract: Provided is a cell analyzer including: a light source unit configured to apply light to test cells each containing first substances which are bound to first fluorescent dyes and which serve as an index for therapeutic strategy judgement; an image capturing unit configured to capture an image of fluorescence caused by the light; a processing unit configured to process the image obtained by the image capturing unit; and a display unit configured to display a process result obtained by the processing unit, wherein the processing unit obtains a first image by performing an inactivation process of quenching the first fluorescent dyes, an activation process of activating a part of the first fluorescent dyes that have been quenched, and an image capturing process of capturing, by means of the image capturing unit, an image of the fluorescence by applying light from the light source unit to each test cell; extracts bright points based on the first fluorescent dyes on the basis of the first image; classifies the extracted bright points into groups each corresponding to one first substance, thereby to obtain the number of the first substances in the test cell on the basis of the number of the classified groups; obtains therapy index information serving as an index for therapeutic strategy judgement, on the basis of the obtained number of the first substances; and causes the display unit to display the obtained therapy index information.

公开(公告)号：US20250043347A1

公开(公告)日：2025-02-06

申请号：US18793236

申请日：2024-08-02

Applicant: KEIO UNIVERSITY ,  HEARTSEED INC. ,  SYSMEX CORPORATION

Inventor： Shugo TOHYAMA ,  Otoya SEKINE ,  Sayaka KANAAMI ,  Kanako MASUMOTO ,  Yuki AIHARA

IPC: C12Q1/6881 ,  C12N5/00 ,  C12N5/077

Abstract: Disclosed is a method for evaluating cell differentiation state, including inducing pluripotent stem cells to differentiate into mesodermal cells in a liquid medium by a first differentiation treatment for inducing pluripotent stem cells to differentiate into mesodermal cells and a second differentiation treatment for inducing the mesodermal cells to differentiate into cardiomyocytes; collecting a supernatant of the liquid medium comprising cells induced to differentiate by the second differentiation treatment; and measuring miRNA-3p in miR-1/133a cluster in the supernatant, where the miRNA-3p is at least one selected from a group consisting of miR-1-3p and miR-133a-3p, and the measured value of miRNA-3p is an index of differentiation into cardiomyocytes.

公开(公告)号：US20200010829A1

公开(公告)日：2020-01-09

申请号：US16425175

申请日：2019-05-29

Applicant: SYSMEX CORPORATION

Inventor： Kanako MASUMOTO ,  Yuki AIHARA

IPC: C12N15/10 ,  C12Q1/6876

Abstract: Disclosed is a method for synthesizing cDNA by mixing a target RNA, a first oligonucleotide molecule, a second oligonucleotide molecule, and a reverse transcriptase, using the target RNA as a template, the first oligonucleotide molecule having a first region that hybridizes to the target RNA at 3′ end, and having a second region that hybridizes to the second oligonucleotide molecule on 5′ side of the first region.