公开(公告)号：US20250043347A1

公开(公告)日：2025-02-06

申请号：US18793236

申请日：2024-08-02

Applicant: KEIO UNIVERSITY ,  HEARTSEED INC. ,  SYSMEX CORPORATION

Inventor： Shugo TOHYAMA ,  Otoya SEKINE ,  Sayaka KANAAMI ,  Kanako MASUMOTO ,  Yuki AIHARA

IPC: C12Q1/6881 ,  C12N5/00 ,  C12N5/077

Abstract: Disclosed is a method for evaluating cell differentiation state, including inducing pluripotent stem cells to differentiate into mesodermal cells in a liquid medium by a first differentiation treatment for inducing pluripotent stem cells to differentiate into mesodermal cells and a second differentiation treatment for inducing the mesodermal cells to differentiate into cardiomyocytes; collecting a supernatant of the liquid medium comprising cells induced to differentiate by the second differentiation treatment; and measuring miRNA-3p in miR-1/133a cluster in the supernatant, where the miRNA-3p is at least one selected from a group consisting of miR-1-3p and miR-133a-3p, and the measured value of miRNA-3p is an index of differentiation into cardiomyocytes.

公开(公告)号：US20200010829A1

公开(公告)日：2020-01-09

申请号：US16425175

申请日：2019-05-29

Applicant: SYSMEX CORPORATION

Inventor： Kanako MASUMOTO ,  Yuki AIHARA

IPC: C12N15/10 ,  C12Q1/6876

Abstract: Disclosed is a method for synthesizing cDNA by mixing a target RNA, a first oligonucleotide molecule, a second oligonucleotide molecule, and a reverse transcriptase, using the target RNA as a template, the first oligonucleotide molecule having a first region that hybridizes to the target RNA at 3′ end, and having a second region that hybridizes to the second oligonucleotide molecule on 5′ side of the first region.

公开(公告)号：US20170186173A1

公开(公告)日：2017-06-29

申请号：US15391311

申请日：2016-12-27

Applicant: Sysmex Corporation ,  National University Corporation Tokyo Medical and Dental University

Inventor： Kazumi HAKAMADA ,  Yuki AIHARA ,  Masaya OKADA ,  Toshiyuki SATO ,  Ichiro SEKIYA ,  Eiji KOBAYASHI

IPC: G06T7/13 ,  G06T7/136 ,  G06T7/00 ,  G06T7/194 ,  G06T7/90 ,  G06K9/00 ,  G06T7/174

Abstract: A method comprises obtaining a first cell image of a cell by a first observation method, obtaining a second cell image of the cell by a second observation method that is different from the first observation method, and determining the region of the cell based on the first cell image and the second cell image.

公开(公告)号：US20150275176A1

公开(公告)日：2015-10-01

申请号：US14673199

申请日：2015-03-30

Applicant: SYSMEX CORPORATION

Inventor： Eiji KOBAYASHI ,  Toshiyuki SATO ,  Kenji AKAMA ,  Nobuyasu HORI ,  Masaki SHIBAYAMA ,  Yuki AIHARA ,  Masakazu KADOWAKI

IPC: C12N5/078 ,  C12N5/077

CPC classification number: C12N5/0644 ,  A01N1/0247 ,  C12N5/0603 ,  C12N5/0607 ,  C12N5/0652 ,  C12N5/0663 ,  C12N5/0679 ,  C12N5/0696 ,  C12N2506/11 ,  C12N2506/1353

Abstract: Disclosed is a method for obtaining differentiated cells and/or differentiated cell products, including the steps of: introducing undifferentiated cells into a perfused organ or living tissue; subjecting the undifferentiated cells introduced to perfusion culture together with the organ or living tissue so as to allow the undifferentiated cells to differentiate, thereby obtaining differentiated cells and/or differentiated cell products; collecting a perfusion culture solution that contains the resulting differentiated cells and/or differentiated cell products; and obtaining the differentiated cells and/or differentiated cell products contained in the collected perfusion culture solution.

Abstract translation: 公开了一种获得分化细胞和/或分化细胞产物的方法，包括以下步骤：将未分化细胞导入灌注的器官或活组织; 使未分化细胞与器官或活组织一起引入灌注培养物，以使未分化细胞分化，从而获得分化细胞和/或分化细胞产物; 收集含有所得分化细胞和/或分化细胞产物的灌注培养液; 并获得收集的灌注培养溶液中所含的分化细胞和/或分化细胞产物。

公开(公告)号：US20180275029A1

公开(公告)日：2018-09-27

申请号：US15935445

申请日：2018-03-26

Applicant: SYSMEX CORPORATION

Inventor： Koya YAMAWAKI ,  Yuki AIHARA

IPC: G01N1/40 ,  G01N33/543

CPC classification number: G01N1/4055 ,  G01N1/4077 ,  G01N33/5432

Abstract: Disclosed is a method for concentrating extracellular vesicles, comprising preparing a mixture comprising a first fraction and a second fraction by mixing a liquid sample comprising extracellular vesicles, a polysaccharide, and a polyether having an average molecular weight of 20,000 or less, wherein the first fraction comprises a higher concentration of extracellular vesicles than the second fraction, and the first fraction comprises a higher concentration of extracellular vesicles than the liquid sample.

公开(公告)号：US20200347452A1

公开(公告)日：2020-11-05

申请号：US16939193

申请日：2020-07-27

Applicant: SYSMEX CORPORATION

Inventor： Yuma OKA ,  Yuki AIHARA

IPC: C12Q1/6881

Abstract: A problem to be addressed is to provide a method that can evaluate the state of undifferentiated cells without destroying cells. The above problem is solved by providing a method for evaluating the state of undifferentiated cells. The method includes: measuring at least one miRNA selected from miR371, miR372, and miR373 in a liquid phase fraction of a liquid that contains undifferentiated cells; and evaluating a state of the undifferentiated cells on the basis of a measurement value of the miRNA.

公开(公告)号：US20190338359A1

公开(公告)日：2019-11-07

申请号：US16514244

申请日：2019-07-17

Applicant: SYSMEX CORPORATION

Inventor： Yuki AIHARA

IPC: C12Q1/6881

Abstract: Provided is a method capable of detecting pluripotent stem cells with enhanced sensitivity without destroying cells after differentiation induction. The present invention provides a method including: measuring miRNAs in a miR302/367 cluster in a liquid phase fraction of a cell culture solution during differentiation induction of pluripotent stem cells and/or after the differentiation induction; and evaluating a differentiation state of the cells in the cell culture solution on the basis of a miRNA measurement value. The present invention provides a method for detecting pluripotent stem cells, including the steps of: measuring miRNAs in a miR302/367 cluster in a liquid phase fraction of a cell culture solution during differentiation induction of pluripotent stem cells and/or after the differentiation induction; and detecting pluripotent stem cells in the cell culture solution on the basis of a miRNA measurement value.