公开(公告)号：US20220228194A1

公开(公告)日：2022-07-21

申请号：US17575924

申请日：2022-01-14

Applicant: SYSMEX CORPORATION

Inventor： Masahiro MIURA ,  Kenji AKAMA ,  Masaaki KAWAI ,  Masaya OKADA ,  Kentaro SHIRAI ,  Yuichiro YOSHIDA ,  Tasuku YOTORIYAMA ,  Michitaka NOTOYA ,  Soichi OUE

IPC: C12Q1/686

Abstract: In an example of an embodiment, a first sample set is prepared that includes a test sample prepared from a first subject sample and a reagent, and at least one control sample prepared from at least one of a positive control and a negative control, and a second sample set is prepared that includes a test sample prepared from a second subject sample and a reagent and does not contain at least one of the control samples contained in the first sample set. The nucleic acid amplification in the first sample set is measured in the first unit, the nucleic acid amplification in the second sample set is measured in the second unit, and the measurement result of each test sample contained in the first sample set and second sample set is analyzed based on the measurement result of the control sample contained in at least the first sample set.

公开(公告)号：US20180345289A1

公开(公告)日：2018-12-06

申请号：US15992921

申请日：2018-05-30

Applicant: SYSMEX CORPORATION

Inventor： Nobuhiro KITAGAWA ,  Yoshinobu MIURA ,  Michitaka NOTOYA ,  Kenichiro SUZUKI

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L2200/143 ,  B01L2300/021

Abstract: Disclosed is a specimen processing chip installed in a liquid feeder, comprising a flow path into which a first liquid and a second liquid flow, a first well having a first injection port into which the first liquid is injected by an operator, and a first liquid feed port for feeding the first liquid injected from the first injection port to the flow path, that is smaller in diameter than the first injection port, a second well having a second injection port into which the second liquid fed from the liquid feeder is injected, and a second liquid feed port for feeding the second liquid injected from the second injection port to the flow path, that is smaller in diameter than the second injection port, and an identification section for distinguishing between the first injection port and the second injection port.

公开(公告)号：US20180246020A1

公开(公告)日：2018-08-30

申请号：US15904972

申请日：2018-02-26

Applicant: SYSMEX CORPORATION

Inventor： Nobuhiro KITAGAWA ,  Yoshinobu MIURA ,  Michitaka NOTOYA ,  Shuji YAMASHITA ,  Nobuyasu HORI

IPC: G01N1/38

CPC classification number: G01N1/38 ,  B01L3/0293 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2400/0487 ,  G01N2001/386

Abstract: Disclosed is a liquid sending method using a sample processing chip having a flow path into which a plurality of liquids flow, and the method includes: sending, into the flow path, a first liquid held in a liquid holding portion provided in the sample processing chip by applying pressure to the liquid holding portion; sending, into the flow path, a second liquid in a storage portion provided in a liquid sending device connected to the sample processing chip, through an injection hole provided in the sample processing chip, by applying pressure to the storage portion; and forming, in the flow path, a fluid containing the first liquid having been sent from the liquid holding portion, and the second liquid having been sent through the injection hole.

公开(公告)号：US20180245145A1

公开(公告)日：2018-08-30

申请号：US15900942

申请日：2018-02-21

Applicant: SYSMEX CORPORATION

Inventor： Michitaka NOTOYA ,  Shuji YAMASHITA ,  Mika YOSHIMURA ,  Yutaka MAEDA

IPC: C12Q1/6848 ,  C12Q1/686

CPC classification number: C12Q1/6848 ,  C12Q1/686 ,  C12Q2521/101 ,  C12Q2525/101 ,  C12Q2537/149

Abstract: Disclosed is a nucleic acid amplification method. The method comprises: performing a first nucleic acid amplification using a target nucleic acid contained in a sample as a template; and performing a second nucleic acid amplification using the amplification product produced in the first nucleic acid amplification step as a template. In the first nucleic acid amplification, a DNA polymerase that selectively amplifies a nucleic acid not comprising a uracil base is used to produce an amplification product that does not comprise a uracil base. In the second nucleic acid amplification, (i) dUTP and/or a primer comprising a uracil base, and (ii) a DNA polymerase capable of amplifying a nucleic acid comprising a uracil base are used to produce an amplification product that comprises a uracil base.