公开(公告)号：US20220228194A1

公开(公告)日：2022-07-21

申请号：US17575924

申请日：2022-01-14

Applicant: SYSMEX CORPORATION

Inventor： Masahiro MIURA ,  Kenji AKAMA ,  Masaaki KAWAI ,  Masaya OKADA ,  Kentaro SHIRAI ,  Yuichiro YOSHIDA ,  Tasuku YOTORIYAMA ,  Michitaka NOTOYA ,  Soichi OUE

IPC: C12Q1/686

Abstract: In an example of an embodiment, a first sample set is prepared that includes a test sample prepared from a first subject sample and a reagent, and at least one control sample prepared from at least one of a positive control and a negative control, and a second sample set is prepared that includes a test sample prepared from a second subject sample and a reagent and does not contain at least one of the control samples contained in the first sample set. The nucleic acid amplification in the first sample set is measured in the first unit, the nucleic acid amplification in the second sample set is measured in the second unit, and the measurement result of each test sample contained in the first sample set and second sample set is analyzed based on the measurement result of the control sample contained in at least the first sample set.

公开(公告)号：US20170073770A1

公开(公告)日：2017-03-16

申请号：US15266385

申请日：2016-09-15

Applicant: SYSMEX CORPORATION

Inventor： Kengo GOTO ,  Yuichiro YOSHIDA ,  Yasuhiro OTOMO

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/158

Abstract: Disclosed is a method for supporting a diagnosis of a risk of colorectal cancer recurrence, including the steps of: performing a first measurement to measure the levels of expression of a plurality of genes selected from a first gene group present in a region from 18q21 to 18q23 on the long arm of chromosome 18 in a biological sample collected from a patient with colorectal cancer, a second measurement to measure the levels of expression of a plurality of genes selected from a second gene group present in a region from 20q11 to 20q13 on the long arm of chromosome 20, and a third measurement to measure the levels of expression of a plurality of genes selected from a third gene group including ANGPTL2, AXL, C1R, C1S, CALHM2, CTSK, DCN, EMP3, GREM1, ITGAV, KLHL5, MMP2, RAB34, SELM, SRGAP2P1, and VIM; and determining the risk of colorectal cancer recurrence of the patient based on the levels of expression measured in the measurement step.

Abstract translation: 本发明公开了一种支持结肠直肠癌复发风险诊断的方法，包括以下步骤：进行第一测定，测定选自18q21〜18q23的区域的第一基因组的多种基因的表达水平 在从患有结肠直肠癌的患者收集的生物样品中的18号染色体的长臂上，测量多个选自20q11至20q13区域的第二基因组的基因表达水平的第二测量 染色体20的臂，以及第三测量，以测量选自包括ANGPTL2，AXL，C1R，C1S，CALHM2，CTSK，DCN，EMP3，GREM1，ITGAV，KLHL5，MMP2的第三基因组的多种基因的表达水平 ，RAB34，SELM，SRGAP2P1和VIM; 并且基于在测量步骤中测量的表达水平来确定患者的结肠直肠癌复发的风险。

公开(公告)号：US20170211138A1

公开(公告)日：2017-07-27

申请号：US15405684

申请日：2017-01-13

Applicant: SYSMEX CORPORATION

Inventor： Tsuyoshi NAKANO ,  Yuichiro YOSHIDA

IPC: C12Q1/68

Abstract: Disclosed is a method of quality control of nucleic acid amplification using quality control oligonucleotide. The method comprises a nucleic acid detection step and a determination step. The nucleic acid detection step comprises the steps of: preparing a nucleic acid sample containing a target nucleic acid and a quality control polynucleotide; preparing a compartment containing one molecule of the target nucleic acid and a compartment containing one molecule of the quality control polynucleotide; and carrying out nucleic acid amplification of the target nucleic acid and the quality control polynucleotide, in the compartments, and carrying out signal detection using a detection probe to detect a signal originated from the detection probe. In the determination step, it is determined as to whether or not the nucleic acid detection step is proper on the basis of the result obtained in the signal detection step.

公开(公告)号：US20140287965A1

公开(公告)日：2014-09-25

申请号：US14298386

申请日：2014-06-06

Applicant: SYSMEX CORPORATION

Inventor： Yuichiro YOSHIDA ,  Masaki KOBAYASHI ,  Yasuhiro OTOMO

IPC: G06F19/20

CPC classification number: G06F19/20 ,  G06F19/00 ,  G06F19/24 ,  G16H50/30 ,  Y02A90/26

Abstract: The invention provides a method for determining presence of a disease, comprising steps of; measuring the levels of expression of transcription products of genes in a biological sample obtained from a subject suspected of having a target disease, wherein the genes comprise at least one gene belonging to each of at least two disease-determining gene families related to the target disease; obtaining values representing deviations by standardizing the levels of the expression based on the levels of expression of transcription products of the corresponding genes in a plurality of healthy subjects; obtaining the average of values representing deviations with respect to the gene belonging to each of the disease-determining gene families; and determining whether or not the subject has the target disease by using the average; as well as a computer program product for determining presence of a disease.

Abstract translation: 本发明提供了一种确定疾病存在的方法，包括以下步骤： 测量从疑似患有目标疾病的受试者获得的生物样品中的基因的转录产物的表达水平，其中所述基因包含至少一种属于至少两种与目标疾病相关的疾病决定基因家族的基因 ; 基于多个健康受试者中相应基因的转录产物的表达水平，通过标准化表达水平来获得代表偏差的值; 获得相对于属于每个疾病决定基因家族的基因的偏差值的平均值; 并通过使用平均值确定受试者是否具有目标疾病; 以及用于确定疾病存在的计算机程序产品。