公开(公告)号：US20180113117A1

公开(公告)日：2018-04-26

申请号：US15791456

申请日：2017-10-24

Applicant: SYSMEX CORPORATION

Inventor： Keiko YOSHIKAWA ,  Yuma OKA ,  Shingo FUJIYAMA

IPC: G01N33/50 ,  G01N15/14 ,  G01N21/64

CPC classification number: G01N33/5041 ,  G01N15/1459 ,  G01N21/6428 ,  G01N33/566 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2333/705

Abstract: A cell information acquisition method may include: binding a binding substance to a receptor on a cell membrane surface of a cell, the binding substance being bindable to the receptor; permeabilizing a cell membrane of the cell after the binding; labeling a receptor in an intracellular area of the cell with a binding substance labeled with a first labeling substance after the permeabilizing; causing a specimen including the cell to flow through a flow path after the labeling; irradiating the cell included in the specimen flowing through the flow path with light; and acquiring a signal based on light generated from the first labeling substance in the cell irradiated with light.

公开(公告)号：US20240424495A1

公开(公告)日：2024-12-26

申请号：US18748459

申请日：2024-06-20

Applicant: SYSMEX CORPORATION

Inventor： Taiga AJIRI ,  Kentaro SHIRAI ,  Shingo FUJIYAMA ,  Hiroyuki OBINATA ,  Natsuki MAEDA

IPC: B01L3/00 ,  B01D29/01 ,  B01D29/90

Abstract: A particle separation chip that separates particles contained in a specimen based on particle size may include a substrate comprising a micro flow path inside of the substrate; and a perforated sheet comprising holes. The micro flow path includes: a main flow path including an inlet arranged at upstream side, into which a specimen comprising a first particle, and a second particle that is smaller than the first particle is supplied; and a first outlet arranged at downstream side, from which a specimen comprising the first particle is discharged; and a branch flow path connected to the main flow path at branches along a flow direction of the main flow path, including a second outlet from which a specimen comprising the second particle is discharged at the downstream side. The main flow path and the branch flow path are connected via the holes of the perforated sheet at the branches.

公开(公告)号：US20190380329A1

公开(公告)日：2019-12-19

申请号：US16552519

申请日：2019-08-27

Applicant: SYSMEX CORPORATION

Inventor： Nobuyasu HORI ,  Shingo FUJIYAMA

IPC: A01N1/02 ,  C12N5/078

Abstract: The present invention relates to a perfusion device and a perfusion method for culturing an undifferentiated cell using an organ or tissue extracted from a living body.